The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Diversity of Endophytic Fungi Isolated from Pinus densiflora and Larix kaempferi in Mt. Oser, Korea

충남 오서산에 분포하는 소나무와 일본잎갈나무에서 분리한 내생균의 다양성

  • Eo, Ju-Kyeong (Department of Biology Education, Korea National University of Education) ;
  • Kim, Chang-Kyun (Department of Biology Education, Korea National University of Education) ;
  • Lee, Hyang Burm (Division of Applied Bioscience & Biotechnology, Chonnam National University) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
  • 어주경 (한국교원대학교 생물교육과) ;
  • 김창균 (한국교원대학교 생물교육과) ;
  • 이향범 (전남대학교 응용생물공학부) ;
  • 엄안흠 (한국교원대학교 생물교육과)
  • Received : 2013.09.02
  • Accepted : 2013.09.16
  • Published : 2013.09.30
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Abstract

Diversity of endophytic fungi of two species associated with host plants, Pinus densiflora and Larix kaempferi in Mt. Oser were investigated. Endophytic fungi were isolated from surface sterilized leaves of the 13 host plants and identified by morphological traits and rDNA ITS sequences analysis. Totally 37 isolates were discovered and identified into 17 taxa. Of them, 59% isolates belonged to Leotiomycetes, 30% isolates Sordariomyetes, 8% isolates Dothideomycetes and 3% isolates Agaricomycetes. Results showed a similar diversity pattern with previous studies. Also, species diversity (H') of endophytic fungi isolated from L. kaempferi was higher than that from P. densiflora. The endophytic species composition showed significant differences between host plants. Particularly, taxon of Lophodermium is a major genus in the host plants. However, critical research of the endophytic species diversities within the genus in Korea is required. The communities of endophytic fungi isolated in this study showed differences in diversities and species composition between host plants.

오서산의 소나무와 일본잎갈나무 두 수종의 침엽에서 내생균의 다양성을 분석하였다. 총 13개체의 숙수식물에서 채집한 침엽을 표면살균하여 분리한 균주들은 형태적인 특징과 rDNA 유전자(ITS 부위) 분석을 수행하였다. 그 결과 총 37개의 균주가 분리되었으며 이들은 17개의 분류군으로 묶을 수 있었다. 그 중 59%는 Leotiomycetes에 속하였으며, 30%는 Sordariomyetes에, 8%는 Dothideomycetes에 속하였으며, 3%는 Agaricomycetes에 속하는 균으로 판명 되었다. 이러한 결과들은 선행연구의 결과들과 매우 유사하였으며 소나무에서보다 일본잎갈나무에서 내생균의 종 다양성이 높게 나타났다. 특히 Lophodermium 속에 속하는 분류군들이 내생균의 다양성에서 주요한 균류로 확인되었으며, 한국에 분포하는 Lophodermium 속 내 종들에 대한 심도 있는 연구가 요구된다.

Keywords

References

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