The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Nitrogen Source-requirement and Preference of Ectomycorrhizal Fungi in Pure Culture

순수배양 시 외생균근균의 질소원 요구성과 선호도

  • Jeon, Sung-Min (Division of Wood Chemistry & Microbiology, Korea Forest Research Institute) ;
  • Ka, Kang-Hyeon (Division of Wood Chemistry & Microbiology, Korea Forest Research Institute)
  • 전성민 (국립산림과학원 화학미생물과) ;
  • 가강현 (국립산림과학원 화학미생물과)
  • Received : 2013.09.09
  • Accepted : 2013.09.18
  • Published : 2013.09.30
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Abstract

The nitrogen source-requirement and preference of ectomycorrhizal fungi (42 strains belong to 13 genera) collected from Korean forests were investigated by incubating mycelial cells in four nitrogen source test media for 56 days. The nitrogen requirement was relatively high in five strains (Heimioporus japonicas (KFRI 1224), Suillus luteus (KFRI 1232), Tylopilus castaneiceps (KFRI 1383), Suillus granulates (KFRI 1997), Rhizopogon sp. (KFRI 1434)) in comparison with other strains. Forty-five percent of ectomycorrhizal strains including Boletus griseus (KFRI 1362) preferred the ammonium-form of inorganic nitrogen source for mycelial growth, whereas thirty-six percent of tested strains including Suillus grevillei (KFRI 1125) preferred the nitrate-form as an inorganic nitrogen source.

국내 산림에서 수집한 외생균근균(13속 42균주)을 질소원이 서로 다른 4종류의 시험배지에 접종하고 56일간 순수 배양하여 질소원 요구성과 선호도를 조사하였다. 그 결과, 일본연지그물버섯(KFRI 1224), 비단그물버섯(KFRI 1232), 끈적쓴맛그물버섯(KFRI 1383), 젖비단그물버섯(KFRI 1997), 알버섯류(KFRI 1434) 등이 질소원 요구성이 높은 것으로 조사되었다. 검은머리그물버섯(KFRI 1362)을 비롯하여 총 시험균주의 45%는 질산염보다 암모늄 형태의 무기질소원을 더 선호하였다. 반면 큰비단그물버섯(KFRI 1125)을 비롯한 총 시험균주의 36%는 질산염 형태의 무기질소원을 더 선호하였다.

Keywords

References

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