The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Identification of Yeasts Isolated from Wild Flowers Collected in Coast Areas of Korea Based on the 26S rDNA Sequences

우리나라 일부 해안 지역 야생화들로부터 분리한 효모들의 분자 생물학적 동정

  • Min, Jin Hong (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Lee, Hyang Burm (Division of Applied Bioscience & Biotechnology, Chonnam National University) ;
  • Lee, Jong Soo (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Kim, Ha Kun (Department of Biomedicinal Science and Biotechnology, Paichai University)
  • 민진홍 (배재대학교 바이오.의생명공학과) ;
  • 이향범 (전남대학교 응용생명공학부) ;
  • 이종수 (배재대학교 바이오.의생명공학과) ;
  • 김하근 (배재대학교 바이오.의생명공학과)
  • Received : 2013.07.19
  • Accepted : 2013.09.18
  • Published : 2013.09.30
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Abstract

Several yeast colonies were isolated from wild flowers collected from East, West and South coast areas of Korea by plating of flower suspensions on the YPD plates containing antibiotics, streptomycin and ampicillin. Polymerase chain reactions (PCR) were performed for the amplification of D1/D2 region of 26S rDNA for those colonies. PCR-amplified nucleotide sequences were compared using BLAST for their identification. As results, 27 yeast strains belonged to 15 species were isolated from wild flowers collected at Donghae, where is located in eastern coast of Korea. Also, 34 strains belonged to 17 species were isolated from wild flowers of Daecheon, where is located in western coast of Korea. In addition, 22 strains belonged to 13 species were isolated from wild flowers collected at Wando, where is located in southern coast of Korea. Among those 45 species isolated from 3 different collection sites, only 4 species including Cryptococcus laurentii, Metschnikowia koreensis, Pseudozyma rugulosa, and Rhodotorula mucilaginosa were found from all 3 different collection sites. And 5 species including Cryptococcus aureus, Cryptococcus flavus, Hanseniaspora uvarum, Pichia guilliermondii, and Rhodosporidium fluviale were overlapped from the at least 2 different collection sites. Other 23 species were found only in a specific collection sites implying that each area has distinctive yeast flora.

국내 자연환경으로부터 다양한 효모들을 분리, 동정하고 나아가 이들로부터 유용물질을 생산하는 효모자원을 확보하기 위한 연구의 일환으로 우리나라 동해안, 서해안, 남해안에 서식하는 야생화들을 채집하여 이들로부터 효모들을 분리한 후 분자생물학적 방법으로 동정하였다. 동해시에서 수집한 야생화로부터는 Candida silvae 등을 포함한 15종에 속하는 27균주의 효모들을 분리하였다. 서해안의 대천시 해수욕장 주위 야생화에서는 Bulleromyces albus를 비롯한 17종 34균주가 분리, 동정되었다. 또한 남해안의 완도군 대문리 주위의 야생화들로부터는 Cryptococcus flavus를 포함하여 13종에 속하는 효모 22 균주들이 분리 동정되었다. 전체적으로 우리나라 동해안, 서해안, 남해안의 야생화로부터 모두 45종에 속하는 효모들 83균주를 분리, 동정하였다.

Keywords

References

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