The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Content Comparative Analysis and Classification for Piniellia ternate, P. pedatisecta and Typhonium flagelliforme by HPLC-PDA analysis

HPLC-PDA를 이용한 반하, 호장남성, 수반하의 분류 및 함량분석

  • Jo, Ji Eun (Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Lee, A Yeong (Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Kim, Hyo Seon (Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Moon, Byeong Cheol (Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Choi, Goya (Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Ji, Yunui (Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Kim, Ho Kyoung (Herbal Medicine Research Group, Korea Institute of Oriental Medicine)
  • 조지은 (한국한의학연구원 한약자원그룹) ;
  • 이아영 (한국한의학연구원 한약자원그룹) ;
  • 김효선 (한국한의학연구원 한약자원그룹) ;
  • 문병철 (한국한의학연구원 한약자원그룹) ;
  • 최고야 (한국한의학연구원 한약자원그룹) ;
  • 지윤의 (한국한의학연구원 한약자원그룹) ;
  • 김호경 (한국한의학연구원 한약자원그룹)
  • Received : 2013.08.15
  • Accepted : 2013.09.22
  • Published : 2013.09.30
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Abstract

Objectives : A quantitative method using high performance liquid chromatography with a photodiode array detector(HPLC-PDA) was established for the quantitative analysis of the four main compound and pattern analysis to classification Piiellia ternate, P. pedatisecta and Typhonium flagelliforme. Methods : The analytical procedure for the determination of P. ternata, together with the known main compounds uracil, uridine, guanosine and adenosine was established. Optimum HPLC-PDA separation of these P. ternata was possible on Luna C18(2) column material, using water and acetonitrile as mobile phase. The method was validated according to regulatory guidelines. In addition, this assay method were analyzed for the content of four main compound in P. ternata, P. pedatisecta and T. flagelliforme and by data obtained from the HPLC-PDA analysis was performed principal component analysis(PCA). Results : Validation results indicated that the HPLC method is well suited for the determination of the roots of P. ternata with a good linearity ($r^2$ > 0.999), precision and recovery rates. Analysis of HPLC-PDA, the average content of uracil, uridine, guanosine and adenosine was significantly higher in P. ternate>P. pedatisecta> T. flagelliforme order. The application of PCA to main compound data by HPLC-PDA permitted the effective discrimination among the three species. Conclusions : Analysis of both HPLC-PDA and PCA confirmed the fact that four main compound and pattern profiles of P. ternata, P. pedatisecta and T. flagelliforme were different from each other.

Keywords

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