Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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The Effect of Rice Bran Extract on the Apoptosis Induction of HL-60 Leukemia Cells

미강(Rice Bran) 추출물의 HL-60 백혈병 세포 Apoptosis 유도 효과

  • Kim, Eun-Ji (Department of Medicine, School of Medicine, Jeju National University) ;
  • Moon, Jungsun (College of Pharmacy, Sahmyook University) ;
  • Kang, Jung-Il (Department of Medicine, School of Medicine, Jeju National University) ;
  • Lee, Young-Ki (Department of Medicine, School of Medicine, Jeju National University) ;
  • Koh, Young-Sang (Department of Medicine, School of Medicine, Jeju National University) ;
  • Yoo, Eun-Sook (Department of Medicine, School of Medicine, Jeju National University) ;
  • Kang, Hee-Kyoung (Department of Medicine, School of Medicine, Jeju National University) ;
  • Yim, Dongsool (College of Pharmacy, Sahmyook University)
  • 김은지 (제주대학교 의학전문대학원 의학과) ;
  • 문정선 (삼육대학교 약학대학 약학과) ;
  • 강정일 (제주대학교 의학전문대학원 의학과) ;
  • 이영기 (제주대학교 의학전문대학원 의학과) ;
  • 고영상 (제주대학교 의학전문대학원 의학과) ;
  • 유은숙 (제주대학교 의학전문대학원 의학과) ;
  • 강희경 (제주대학교 의학전문대학원 의학과) ;
  • 임동술 (삼육대학교 약학대학 약학과)
  • Received : 2013.04.23
  • Accepted : 2013.08.07
  • Published : 2013.09.30
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Abstract

In this study, we investigated the anticancer effect of rice bran extract in HL-60 human promyelocytic leukemia cells. The extract of rice bran inhibited the proliferation of HL-60 cells. When treated with the rice bran extract, we could observe the apoptotic characteristics such as apoptotic bodies and the increase of sub-G1 hypodiploid cell population, increase of Bax level, decrease of Bcl-2 expression, cleavage of procaspase-3, cleavage of procaspase-9 and cleavage of poly(ADP-ribose) polymerase(PARP) in HL-60 cells. Furthermore, the apoptosis induction of HL-60 cells treated with the rice bran extract was also accompanied by the inactivation of mitogen-activated protein kinases (MAPK) such as ERK1/2 MAPK and p38 MAPK. In addition, the rice bran extract induced the down-regulation of c-myc. These data suggested that the rice bran extract could induce the apoptosis via the inactivation of ERK1/2 MAPK and p38 MAPK, and the down-regulation of c-myc in HL-60 acute pomyelocytic leukemia cells. The results support that the rice bran extract might have potential for the treatment of acute promyelocytic leukemia.

Keywords

References

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