Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characterization of a Blend-Biosurfactant of Glycolipid and Lipopeptide Produced by Bacillus subtilis TU2 Isolated from Underground Oil-Extraction Wastewater

  • Cheng, Fangyu (Department of Chemical Engineering, Tsinghua University) ;
  • Tang, Cheng (Department of Chemical Engineering, Tsinghua University) ;
  • Yang, Huan (Department of Chemical Engineering, Tsinghua University) ;
  • Yu, Huimin (Department of Chemical Engineering, Tsinghua University) ;
  • Chen, Yu (Department of Chemical Engineering, Tsinghua University) ;
  • Shen, Zhongyao (Department of Chemical Engineering, Tsinghua University)
  • Received : 2012.09.07
  • Accepted : 2012.11.07
  • Published : 2013.03.28
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Abstract

Biosurfactants have versatile properties and potential industrial applications. A new producer, B. subtilis TU2, was isolated from the underground oil-extraction wastewater of Shengli Oilfield, China. Preliminary flask culture showed that the titer of biosurfactant obtained from the broth of TU2 was ~1.5 g/l at 48 h (718 mg/l after purification), with a reduced surface tension of 32.5 mN/m. The critical micelle concentration was measured as 50 mg/l and the surface tension maintained stability in solution with 50 g/l NaCl and 16 g/l $CaCl_2$ after 5 days of incubation at $70^{\circ}C$. FT-IR spectra exhibited the structure information of both glycolipid and lipopeptide. MALDI-TOF-MS analyses confirmed that the biosurfactant produced by B. subtilis TU2 was a blend of glycolipid and lipopeptide, including rhamnolipid, surfactin, and fengycin. The blended biosurfactant showed 86% of oil-washing efficiency and fine emulsification activity on crude oil, suggesting its potential application in enhanced oil recovery.

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References

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