Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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1-Deoxynojirimycin Isolated from a Bacillus subtilis Stimulates Adiponectin and GLUT4 Expressions in 3T3-L1 Adipocytes

  • Lee, Seung-Min (Department of Food and Nutrition, College of Human Ecology, Yonsei University) ;
  • Do, Hyun Ju (Department of Food and Nutrition and Institute of Health Sciences, Korea University) ;
  • Shin, Min-Jeong (Department of Food and Nutrition and Institute of Health Sciences, Korea University) ;
  • Seong, Su-Il (R&D Center for Life Science, Biotopia Co., Ltd.) ;
  • Hwang, Kyo Yeol (R&D Center for Life Science, Biotopia Co., Ltd.) ;
  • Lee, Jae Yeon (R&D Center for Life Science, Biotopia Co., Ltd.) ;
  • Kwon, Ohsuk (Systems and Synthetic Biology Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jin, Taewon (Cardiovascular Product Evaluation Center, Cardiovascular Research Institute, Yonsei University College of Medicine) ;
  • Chung, Ji Hyung (Cardiovascular Product Evaluation Center, Cardiovascular Research Institute, Yonsei University College of Medicine)
  • Received : 2012.09.14
  • Accepted : 2013.01.02
  • Published : 2013.05.28
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Abstract

We have demonstrated that 1-deoxynojirimycin (DNJ) isolated from Bacillus subtilis MORI could enhance the levels of adiponectin and its receptors in differentiated 3T3-L1 adipocytes, which has been shown to be effective in lowering blood glucose levels and enhancing insulin sensitivity. DNJ was not toxic to differentiated 3T3-L1 adipocytes for up to a concentration of $5{\mu}M$. In terms of expression levels of adiponectin and its receptors (AdipoR1 and AdipoR2), DNJ in concentrations as low as $0.5{\mu}M$ elevated both mRNA and protein levels of adiponectin and transcript levels of AdipoR1 and AdipoR2. In addition, DNJ increased phosphorylation of 5' adenosine monophosphate-activated protein kinase (AMPK) in a statistically significant manner. Finally, treatment with DNJ resulted in increased mRNA expression of glucose transporter 4 (GLUT4), which encodes for a glucose transporter, along with a significant increase in glucose uptake into the adipocytes based on results of a 2-deoxy-D-[$^3H$] glucose uptake assay. Our findings indicate that DNJ may greatly facilitate glucose uptake into adipose tissues by increasing the action of adiponectin via its up-regulated expression as well as its receptor genes. In addition, the glucose-lowering effects of DNJ may be achieved by an increased abundance of GLUT4 protein in the plasma membrane, as a consequence of the increased transcript levels of the GLUT4 gene and the activation of AMPK.

Keywords

References

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