Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Sequential hepatic ultrastructural changes and apoptosis in rabbits experimentally infected with Korean strain of rabbit hemorrhagic disease virus (RHDVa)

국내 분리 토끼출혈병 바이러스(RHDVa)를 감염시킨 토끼 간장에서의 경시적인 초미세구조 변화와 apoptosis

  • Park, Jung-Won (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Chun, Ji-Eun (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Bak, Eun-Jung (Oral Cancer Research Institute, College of Dentistry, Yonsei University) ;
  • Kim, Han (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Lee, Myeong-Heon (Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Hwang, Eui-Kyung (Department of Animal Science, College of Life Science and Natural Resources, Sangji University) ;
  • Kim, Jae-Hoon (College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University) ;
  • Lee, Chung-Bok (College of Veterinary Medicine, Konkuk University) ;
  • Woo, Gye-Hyeong (Department of Clinical Laboratory Science, Semyung University)
  • Received : 2012.08.28
  • Accepted : 2012.12.11
  • Published : 2013.03.31
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Abstract

In this study, to understand the pathogenesis of new rabbit hemorrhagic disease virus (RHDVa) serotype, we carried out to administrate RHDVa to rabbits, and to examine sequential electron microscopic changes and relationship between pathogenesis and apoptosis. TUNEL-positive cells began to be observed from 24 hours after inoculation (HAI) and the number of positive cells was slightly increased with the course of time. Whereas marked increase of positive cells was seen in the liver from the rabbits died acutely. Typical viral particles with cup-like projections and a diameter of 30~40 nm were detected in homogenized liver samples and tissues at 36 and 48, and 48 HAI, respectively. Ultrastructurally, glycogen deposition was observed from the first stage of hepatocellular degeneration by RHDVa infection and then, swelling and disruption of cristae of mitochondria by viral particles, swelling of smooth endoplasmic reticulum, vacuoles and vesicles were detected. Condensation, margination and fragmentation of chromatin were observed in degenerative hepatocytes at 36 and 48 HAI, indicating apoptotic bodies. These data offer that hepatocytic apoptosis by RHDV infection could be closely related with mitochondrial impairment in the hepatocytes.

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References

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