Journal of Ginseng Research

  • 제37권4호
  • /
  • Pages.389-400
  • /
  • 2013
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  • 1226-8453(pISSN)
  • /
  • 2093-4947(eISSN)
고려인삼학회 (The Korean Society of Ginseng)
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Inhibitory effect of Korean Red Ginseng on melanocyte proliferation and its possible implication in GM-CSF mediated signaling

  • Oh, Chang Taek (Department of Genetic Engineering, College of Life Science, Kyung Hee University) ;
  • Park, Jong Il (Department of Genetic Engineering, College of Life Science, Kyung Hee University) ;
  • Jung, Yi Ra (Department of Genetic Engineering, College of Life Science, Kyung Hee University) ;
  • Joo, Yeon Ah (Department of Genetic Engineering, College of Life Science, Kyung Hee University) ;
  • Shin, Dong Ha (Department of Genetic Engineering, College of Life Science, Kyung Hee University) ;
  • Cho, Hyoung Joo (Department of Genetic Engineering, College of Life Science, Kyung Hee University) ;
  • Ahn, Soo Mi (Skin Biotechnology Center, Kyung Hee University) ;
  • Lim, Young-Ho (KGC R&D Headquarters) ;
  • Park, Chae Kyu (KGC R&D Headquarters) ;
  • Hwang, Jae Sung (Department of Genetic Engineering, College of Life Science, Kyung Hee University)
  • 투고 : 2013.03.02
  • 심사 : 2013.04.15
  • 발행 : 2013.10.15
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초록

Korean Red Ginseng (KRG) has been reported to exert anticancer, anti-oxidant, and anti-inflammatory effects. However, there has been no report on the effect of KRG on skin pigmentation. In this study, we investigated the inhibitory effect of KRG on melanocyte proliferation. KRG extract (KRGE) at different concentrations had no effect on melanin synthesis in melan-A melanocytes. Saponin of KRG (SKRG) inhibited melanin content to 80% of the control at 100 ppm. Keratinocyte-derived factors induced by UV-irradiation were reported to stimulate melanogenesis, differentiation, proliferation, and dendrite formation. In this study, treatment of melan-A melanocytes with conditioned media from UV-irradiated SP-1 keratinocytes increased melanocyte proliferation. When UV-irradiated SP-1 keratinocytes were treated with KRGE or SKRG, the increase of melanocyte proliferation by the conditioned media was blocked. Granulocyte-macrophage colony-stimulating factor (GM-CSF) was produced and released from UV-irradiated keratinocytes. This factor has been reported to be involved in regulating the proliferation and differentiation of epidermal melanocytes. In this study, GM-CSF was significantly increased in SP-1 keratinocytes by UVB irradiation ($30mJ/cm^2$), and the proliferation of melan-A melanocytes increased significantly by GM-CSF treatment. In addition, the proliferative effect of keratinocyte-conditioned media on melan-A melanocytes was blocked by anti-GM-CSF treatment. KRGE or SKRG treatment decreased the expression of GM-CSF in SP-1 keratinocytes induced by UVB irradiation. These results demonstrate that UV irradiation induced GM-CSF expression in keratinocytes and KRGE or SKRG inhibited its expression. Therefore, KRG could be a good candidate for regulating UV-induced melanocyte proliferation.

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