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Discrimination of Korean Ginseng Cultivars by Sequence Tagged Sites (STS) Markers

STS 마커를 이용한 고려인삼 품종 및 육성계통 판별

  • Jo, Ick Hyun (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Shin, Mi Ran (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Young Chang (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Lee, Seung Ho (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Jang Uk (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Moon, Ji Young (Experiment Research Institute of National Agricultural Products Quality Management Service, MIFAFF) ;
  • Noh, Bong Soo (Department of Food Science and Technology, Seoul Women's University) ;
  • Kang, Sung Taek (Department of Crop Science and Biotechnology, Dankook University) ;
  • Lee, Dong Jin (Department of Crop Science and Biotechnology, Dankook University) ;
  • Hyun, Dong Yun (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Dong Hwi (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Kee Hong (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Bang, Kyong Hwan (Department of Herbal Crop Research, NIHHS, RDA)
  • 조익현 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 신미란 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김영창 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이승호 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김장욱 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 문지영 (국립농산물품질관리원 시험연구소) ;
  • 노봉수 (서울여자대학교 식품공학과) ;
  • 강성택 (단국대학교 식량생명공학과) ;
  • 이동진 (단국대학교 식량생명공학과) ;
  • 현동윤 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김동휘 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김기홍 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 방경환 (농촌진흥청 국립원예특작과학원 인삼특작부)
  • Received : 2013.09.04
  • Accepted : 2013.09.24
  • Published : 2013.10.30

Abstract

Korean ginseng (P. ginseng C. A. Meyer) is one of the most important medicinal plant in the world. Understanding genetic variability among the assortment of Korean ginseng is important for breeding. The aim of this study was to molecularly characterize Korean ginseng cultivar and breeding lines through the use of eight previously reported STS markers (MFGp183, MFGp130, MFGp110, UFGp74, UFGp163, MFGp108, MFGp81 and UFGp156). All STS markers produced interpretable electropherograms from 31 accessions consisting of 11 Korean ginseng cultivars and 20 breeding lines. When eight STS markers were combined, we identified to total 19 genetic patterns; in particular, nine cultivars (Chunpoong, Yunpoong, Gopoong, Gumpoong, Sunpoong, Sunone, Cheongseon, Sunhyang, Cheonryang) and 5 breeding lines (G08012, G04079, G04075, G08036, G04110) in ginseng samples can be discriminated from the others. Together with other available markers, these STS markers will contribute to the management of ginseng genetic resources and the protection of breeders' rights.

Keywords

References

  1. Bang KH, Lee JW, Kim YC, Kim DH, Lee EH and Jeung JU. (2010). Construction of genomic DNA library of Korean ginseng(Panax ginseng C. A Meyer) and development of sequence-tagged sites. Biological and Pharmaceutical Bulletin 33:1579-1588. https://doi.org/10.1248/bpb.33.1579
  2. Bang KH, Chung JW, Kim YC, Lee JW, Jo IH, Seo AY, Kim OT, Hyun DY, Kim DH and Cha SW. (2011a). Development of SSR markers for identification of Korean ginseng(Panax ginseng C. A. Mey.) cultivars. Korean Journal of Medicinal Crop Science. 19:185-190. https://doi.org/10.7783/KJMCS.2011.19.3.185
  3. Bang KH, Seo AY, Chung JW, Kim YC, Jo IH, Kim JU, Kim DH, Cha SW, Cho YG and Kim HS. (2011b). Analysis of genetic polymorphism and relationship of Korean ginseng cultivars and breeding lines using EST-SSR marker. Korean Journal of Medicinal Crop Science. 20:277-285.
  4. Blake TK, Kadyrzhanova D, Shepard KW, Islam AKMR, Langridge PL, McDonald CL, Erpelding J, Larson S, Blake NK and Talbert LE. (1996). STS-PCR markers appropriate for wheat-barley introgression. Theoretical and Applied Genetics. 93:826-832.
  5. Cheo YW, Park DY, Shin HK, Kwon YS, Yoon HM, Moon JY and Park HY. (2002). Studies for similarity evaluation of radish and chinese cabbage cultivars. Korean Journal of Horticultural Science and Technology. 20:160-167.
  6. Choi KT, Lee CH and Chen SR. (1979). Studies on the variation of flowering date in Korean ginseng plants. Journal of Ginseng Research. 3:35-39.
  7. Chung YY, Lee MG, Chung CM and Jo JS. (1992). The comparison of growth and morphological characteristics among the Korean ginseng, the American ginseng and the bamboo ginseng. Journal of Ginseng Research. 22:147-153.
  8. Erpelding JE, Blake NK, Blake TK and Talbert LE. (1996). Transfer of sequence tagged site PCR markers between wheat and barley. Genome. 39:802-810. https://doi.org/10.1139/g96-101
  9. Hu SY. (1976). The genus Panax(ginseng) in Chinese medicine. Economic Botany. 30:11-28. https://doi.org/10.1007/BF02866780
  10. Huang GQ, Hua YW, Gao Z and Huang HS. (2010). Construction of a methylation filtration library in Hevea brasiliensis. Yi Chuan. 32: 1071-1076.
  11. Hwang TY, Jung SM, Lee SK, Park HM, Jeong KH, Lee YY, Kim SL, Yun HT, Lee JE, Kim DW, Jung GH, Kwon YU, Kim HS and Kim YH. (2012). Discrimination of 110 Korean soybean cultivars by sequence tagged sites(STS)-CAPS markers. Korean Journal of Breeding Science. 44:258-272.
  12. International Union for the Protection of New Varieties of plants(UPOV). (2005). Guidelines for the conduct of tests for distinctness, uniformity and stability. Ginseng. UPOV Geneva, Swiss. TG/224/1.
  13. Kwon WS, Moon CH, Kim YT, Lee MG and Choi KT. (1991). Comparisons of growth, crude saponin, ginsenosides, and anthocyanins in superior lines of Panax ginseng C. A. Meyer. Journal of Ginseng Research. 23:11-17.
  14. Kwon WS, Lee MG and Choi KT. (2000). Breeding process and characteristics of Yunpoong, a new variety of Panax ginseng C. A. Meyer. Journal of Ginseng Research. 24:1-7.
  15. Kwon WS, Lee MG and Lee JH. (2001). Characteristics of flowering and fruiting in new varieties and lines of Panax ginseng C. A. Meyer. Journal of Ginseng Research. 25:41-44.
  16. Kwon WS, Lee JH, Park CS and Yang DC. (2003). Breeding process and characteristics of Gopoong, a new variety of Panax ginseng C. A. Meyer. Journal of Ginseng Research. 27:86-91. https://doi.org/10.5142/JGR.2003.27.2.086
  17. Lee SJ, Jeung JU, Cho SK, Um BY, Chung WI, Bae JM and Shin JS. (2002). Diversity and varietal classification of Hibiscus syriacus L. with the heterogeneity within retrotransposon-like elements. Molecules and Cells. 13:362-368.
  18. Li X, Gardner DR, Ralphs MH and Wang RRC. (2002). Development of STS and CAPS markers for identification of three tall larkspurs(Delphinium spp.). Genome. 45:229-235. https://doi.org/10.1139/g01-149
  19. Lu BR, Cai X and Xin J. (2009). Efficient indica and japonica rice identification based on the InDel molecular method: Its implication in rice breeding and evolutionary research. Progress in Natural Science. 19:1241-1251. https://doi.org/10.1016/j.pnsc.2009.01.011
  20. Olson M, Hood L, Cantor C and Botstein D. (1989). A common language for physical mapping of human genome. Science. 245:1434-1435. https://doi.org/10.1126/science.2781285
  21. Palmer LE, Rabinowicz PD, O'' Shaughnessy AL, Balija VS, Dike S LU, de la Bastide M, Martienssen RA and McCombie WR. (2003). Maize genome sequencing by methylation filtration. Science. 302:2115-2117. https://doi.org/10.1126/science.1091265
  22. Rabinowicz PD. (2003). Constructing gene-enriched plant genomic libraries using methylation filtration technology. Methods in Molecular Biology. 236:21-36.
  23. Steele KA, Ogden R, McEwing R, Briggs H and Gorham J. (2008). InDel markers distinguish Basmatis from other fragrant rice varieties. Field Crops Research. 105:81-87. https://doi.org/10.1016/j.fcr.2007.08.001
  24. Talbert LE, Blake NK, Chee PW, Blake TK and Magyar GM. (1994). Evaluation of “sequence-tagged-site” PCR products as molecular markers in wheat. Theoretical and Applied Genetics. 87:789-794.
  25. Timko MP, Rushton PJ, Laudeman TW, Bokowiec MT, Chipumuro E, Cheung F, Town CD and Chen X. (2008). Sequencing and analysis of the gene-rich space of cowpea. BMC Genomics. 9:103. https://doi.org/10.1186/1471-2164-9-103
  26. Whitelaw CA, Barbazuk WB, Pertea G, Chan AP, Cheung F, Lee Y, Zheng L, Van Heeringen S, Karamycheva S, Bennetzen JL, SanMiguel P, Lakey N, Bedell J, Yuan Y, Budiman MA, Resnick A, Van Aken S, Utterback T, Riedmuller S, Williams M, Feldblyum T, Schubert K, Beachy R, Fraser CM and Quackenbush J. (2003). Enrichment of gene-coding sequences in maize by genome filtration. Science. 302:2118-2120. https://doi.org/10.1126/science.1090047
  27. Yamamoto N, Tsugane T, Watanabe M, Yano K, Maeda F, Kuwata C, Torki M, Ban Y, Nishimura S and Shibata D. (2005). Expressed sequence tags from the laboratory-grown miniature tomato(Lycopersicon esculentum) cultivar Micro-Tom and mining for single nucleotide polymorphisms and insertions/deletions in tomato cultivars. Gene. 356:127-134. https://doi.org/10.1016/j.gene.2005.04.026

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