Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Physiological Changes of Saccharomyces cerevisiae by High Voltage Pulsed Electric Field Treatments

고전압 펄스 전기장 처리에 의한 Saccharomyces cerevisiae의 생리적 변화

  • Park, Hee Ran (Department of Traditional Food Industry, JeonJu University) ;
  • Yoon, So Jung (Department of Traditional Food Industry, JeonJu University) ;
  • Park, Han-Sul (Department of Traditional Food Industry, JeonJu University) ;
  • Shin, Jung-Kue (Department of Korean Cuisine, JeonJu University)
  • 박희란 (전주대학교 전통식품산업학과) ;
  • 윤소정 (전주대학교 전통식품산업학과) ;
  • 박한설 (전주대학교 전통식품산업학과) ;
  • 신정규 (전주대학교 한식조리학과)
  • Received : 2013.02.13
  • Accepted : 2013.06.22
  • Published : 2013.10.31
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Abstract

High voltage pulsed electric fields (PEF) treatment is a promising non-thermal processing technology that can replace or partially substitute for thermal processes. The aim of this research was to investigate the microbial inactivation mechanisms by PEF treatment in terms of physiological changes to Saccharomyces cerevisiae. PEF was applied at the electric field strength of 50 kV/cm, treatment time of 56 ${\mu}s$ and temperature of $40^{\circ}C$. The microbial cells treated with PEF showed loss of salt tolerance on the cell membrane and collapse of the relative pH gradient on in-out of cells. Cell death or injury resulted from the breakdown of homeostasis, decreased $H^+$-ATPase activity, and loss of glycolysis activity.

고전압 펄스 전기장 기술은 가열 공정을 대체할 수 있는 비가열 살균기술로서 꾸준한 연구가 이루어지고 있는 기술이다. 본 실험에서는 미생물의 생리적 측면에서 고전압 펄스 전기장 처리에 의한 효모의 생리적 변화를 살펴보았다. 내염성의 변화를 측정한 결과 사멸하지 않은 세포의 90-99% 이상이 세포 손상에 의해 내염성을 소실하였으며, 내염성을 회복하는데 약 4-6시간의 시간이 걸렸다. 고전압 펄스 전기장 처리 초기에는 세포 내외의 pH 구배(${\Delta}pH$)가 차이가 컸으나 처리시간이 증가함에 따라 그 차이가 감소하였다. 이는 세포의 pH 항상성을 유지시키는 $H^+$-traslocation 기능을 담당하고 있는 $H^+$-ATPase 활성이 소실되었거나 세포막 자체가 손상되어 pH 항상성을 잃어버렸기 때문으로, 이는 고전압 펄스 전기장 처리한 세포의 $H^+$-ATPase의 활성이 초기부터 크게 감소하는 것으로 확인할 수 있었다. 또한 세포의 해당활성이 고전압 펄스 전기장 처리에 의해 크게 감소하여 세포의 대사 기능이 손상되는 것을 확인하였다.

Keywords

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