Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Effect of Daebo (Castanea crenata) Inner Skin Extract on TMT-induced Learning and Memory Injury

TMT 유도성 인지 기능 상실에 대한 대보(밤 품종) 내피 추출물의 효과

  • Kim, Hyeon-Ju (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Jeong, Ji Hee (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Jo, Yu Na (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Jin, Dong Eun (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Jin, Su Il (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Man-Jo (Department of Special Purpose Trees, Korea Forest Research Institute) ;
  • Heo, Ho Jin (Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University)
  • 김현주 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 정지희 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 조유나 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 진동은 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 진수일 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 김만조 (산림과학원 특용자원연구과) ;
  • 허호진 (경상대학교 응용생명과학부, 농업생명과학연구원)
  • Received : 2013.04.15
  • Accepted : 2013.06.30
  • Published : 2013.10.31
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Abstract

The aim of this study was to investigate the anti-amnesic effect of daebo (Castanea crenata) extract on trimethyltin chloride (TMT)-induced learning and memory impairment, in vivo. The inner skin of daebo was extracted using distilled water under reflux conditions. At the end of the adaptation period, ICR mice were divided into a control group, a TMT injection group (negative control), and a sample group (C5: 5 mg/kg body weight; C10: 10 mg/kg body weight; and C20: 20 mg/kg body weight), and were tested with learning and memory tests. The ethylacetate fraction of the daebo inner skin extract was found to increase TMT-induced memory deficit in the Y-maze and passive avoidance test. Brain tissue analysis showed that the ethylacetate fraction of daebo extract lowered the acetylcholine esterase (AChE) activity and malondialdehyde (MDA) content of neuronal cells, both of which are indicative of lipid peroxidation.

본 연구에서는 국내 대표 밤 품종인 대보(Daebo)의 내피를 활용하여 TMT 유도성 인지 기능 상실에 대한 개선 효과를 연구하였다. 실험에서 각 농도별(5, 10, 20 mg/kg of body weight)로 대보 내피 에틸아세테이트 분획물을 섭취한 mouse를 TMT로 인지기능 손상을 유발하여 Y-maze test와 passive avoidance test한 결과, Y-maze test에서 분획물을 섭취한 group이 TMT 단독 처리군과 비교하였을 때 공간 인지기능을 개선시켰고, passive avoidance test 또한 latency time이 증가한 것으로 나타나 TMT에 의해 유발되는 뇌 신경독성 동물 모델로부터 기억 및 학습능력 개선 효과를 갖는 것으로 확인되었다. 또한 in vivo 동물 실험 후 mouse로부터 적출된 whole brain tissue를 대상으로 ex vivo AChE 활성 및 MDA 함량측정 실험한 결과, 에틸아세테이트 분획물이 TMT효과 대비 AChE의 활성을 일부 유의적으로 억제하는 것을 알 수 있었다. 결론적으로 대보 내피 에틸아세테이트 분획물은 신경전달물질인 AChE의 활성을 저해하고 뇌 신경세포 보호 효과를 통하여 인지기능 개선 효과를 유도할 수 있는 것으로 사료된다.

Keywords

References

  1. Son CH, Seok HD, Min KT. Trend and future of the domestic industry for chestnut. Korea Rural Economics Institute, Seoul, Korea. pp. 77-79 (2003)
  2. Kim JS, Jung BH, Joo RW, Choi SI. Marketing of chestnut and economic analysis of chestnut cultivation. Korean J. Forest Econ. 12: 12-21 (2004)
  3. Seo IW, Nam HJ, Kim EH, Shin HS. Determination of polycyclic aromatic hydrocarbons in processed chestnut products. Korean J. Food Sci. Technol. 41: 339-344 (2009)
  4. Hwang TY, Kim JH, Kim JK, Moon KD. The effects of microwave heating of the texture of sugared chestnuts. Korean J. Food Sci. Technol. 30: 569-573 (1998)
  5. Park IS, Kim SK, Kim CS. Physicochemical properties of chestnut starch. J. Korean Agr. Chem. Soc. 25: 218-223 (1982)
  6. Kim SK, Jeon YJ, Kim YT, Lee BJ, Kang OJ. Physicochemical and textural properties of chestnut starches. J. Korean Soc. Food Nutr. 24: 594-600 (1995)
  7. Ha BS, Bae MS, Jeong TM, Sung NJ, Son YO. Studies on constituent variation during storage after freeze-drying of chestnut. Korean J. Food Sci. Technol. 14: 97-105 (1982)
  8. Hwang JY. Changes in quality characteristics of peeled chestnuts with storage temperature. Korean J. Food Nutr. 24: 71-78 (2011) https://doi.org/10.9799/ksfan.2011.24.1.071
  9. Kim YD, Cho DB, Kim KJ, Kim KM, Hur CK, Cho IK. Antimicrobial activity of the solvent extracts from of chestnut. Korean J. Food Preserv. 12: 257-262 (2005)
  10. Kim MJ. Characteristics and cultivation management about chestnut new varieties as daebo, daehan and mipung. National Forestry Cooperative Federation, Seoul, Korea. pp. 81-85 (2010)
  11. Snow RE, Arnold SE. Psychosis in neurodegenerative disease. Semin. Clin. Neuropsychiatry 1: 282-293 (1996)
  12. Kidd PM. Alzheimer's disease, amnestic mild cognitive impairment, and age-associated memory impairment: Current understanding and progress toward integrative prevention. Altern. Med. Rev. 13: 85-115 (2008)
  13. Whitehouse PJ, Price DL, Clark AW, Coyle JT, DeLong MR. Alzheimer's disease: Evidence for selective loss of cholinergic neurons in the nucleus basalis. Ann. Neurol. 10: 122-126 (1981) https://doi.org/10.1002/ana.410100203
  14. Coyle JT, Price DL, DeLong MR. Alzheimer's disease: A disorder of cortical cholinergic innervation. Science 219: 1184-1190 (1983) https://doi.org/10.1126/science.6338589
  15. Jeong HR, Jo YN, Jeong JH, Jin DE, Song BG, Choi SJ, Shin DH, Heo HJ. Antiamnesic effects of ethyl acetate fraction from chestnut (Castanea crenata var. dulcis) inner skin on $A{\beta}_{25-35}$-induced cognitive deficits in mice. J. Med. Food 15: 1051-1056 (2012) https://doi.org/10.1089/jmf.2011.2101
  16. Kim MJ, Choi SJ. Lim ST, Kim HK, Heo HJ. Kim EK, Jun WJ, Cho HY, Kim YJ, Shin DH. Ferulic acid supplementation prevents trimethyltin-induced cognitive deficits in mice. Biosci. Biotech. Bioch. 71: 1063-1068 (2007) https://doi.org/10.1271/bbb.60564
  17. Ellman GL, Courtney KD, Andres V, Featherstone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 7: 88-95 (1961) https://doi.org/10.1016/0006-2952(61)90145-9
  18. Choi SJ, Kim MJ, Heo HJ, Kim JK, Jun WJ, Kim HK, Kim EK, Kim MO, Cho HY, Hwang HJ, Kim YJ, Shin DH. Ameliorative effect of 1.2-benzenedicarboxylic acid dinonyl ester against amyloid beta peptide-induced neurotoxicity. Amyloid 16: 15-24 (2009) https://doi.org/10.1080/13506120802676997
  19. Sarter M, Bodewitz G, Stephens DN. Attenuation of scopolamine induced impairment of spontaneous alternation behavior by antagonist but not inverse agonist and beta-carbolines. Psychopharmacol. 94: 491-495 (1988) https://doi.org/10.1007/BF00212843
  20. LeDoux JE. Emotional memory system in the brain. Behav. Brain Res. 20: 69-79 (1993)
  21. Choi GN, Kim JH, Kwak JH, Jeong CH, Jeong HR, Lee U, Heo HJ. Effect of quercetin on learning and memory performance in ICR mice under neurotoxic trimethyltin exposure. Food Chem. 132: 1019-1024 (2012) https://doi.org/10.1016/j.foodchem.2011.11.089
  22. Talesa VN. Acetylcholinesterase in alzheimer's disease. Mech. Aging Dev. 122: 1961-1969 (2001) https://doi.org/10.1016/S0047-6374(01)00309-8
  23. Earley B, Burke M, Leonard BE. Behavioural, biochemical and histological effects of trimethytin (TMT) induced brain damage in the rat. Neurochem. Int. 21: 351-366 (1992) https://doi.org/10.1016/0197-0186(92)90186-U
  24. Loullis CC, Dean RL, Lippa AS, Clody DE, Coupet J. Hippocampal muscarinic receptor loss following trimethyl tin administration. Pharmacol. Biochem. Behav. 22: 147-151 (1985) https://doi.org/10.1016/0091-3057(85)90498-8
  25. Kim JK, Bae H, Kim MJ, Choi SJ, Cho HY, Hwang HJ, Kim YJ, Lim ST, Kim EK, Kim HK, Kim BY, Shin DH. Inhibitory effect of poncirus trifoliate on acetylcholinesterase and attenuating activity against trimethyltin-induced learning and memory impairment. Biosci. Biotech. Bioch. 73: 1105-1112 (2009) https://doi.org/10.1271/bbb.80859
  26. Kim JK, Choi SJ, Bae H, Kim CR, Cho HY, Kim YJ, Lim ST, Kim CJ, Kim HK, Peterson S, Shin DH. Effects of methoxsalen from poncirus trifoliata on acetylcholinesterase and trimethyltininduced learning and memory impairment. Biosci. Biotech. Bioch. 75: 1984-1989 (2011) https://doi.org/10.1271/bbb.110386
  27. Chang NS, Ryu SM. Antioxidative effects of green tea power diet against ethanol-induced oxidative damage in rat brain regions. J. Nutr. Health 34: 525-531 (2001)

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