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섬유아세포(CCD-986sk)에서 청미래덩굴의 pro-collagen type-1 활성 및 MMP-1의 유전자 발현 저해능

Inhibitory Efficacy of Smilax china L. on Pro-collagen Type-1 Activity and MMP-1 Gene Expression in Fibroblasts (CCD-986sk)

  • 이수연 (호서대학교 한방화장품과학과) ;
  • 이진영 (호서대학교 한방화장품과학과)
  • Lee, Soo-Yeon (Department of Herbal Cosmetic Science, Hoseo University) ;
  • Lee, Jin-Young (Department of Herbal Cosmetic Science, Hoseo University)
  • 투고 : 2013.07.08
  • 심사 : 2013.10.23
  • 발행 : 2013.10.30

초록

청미래덩굴 추출물의 주름개선 효과를 확인하기 위해 elastase 저해활성을 측정한 결과 청미래덩굴 에탄올 추출물과 열수 추출물이 $1,000{\mu}g/ml$에서 각각 41.1%, 16.3%의 활성을 나타내었으며, collagenase 저해활성 측정결과 청미래덩굴 에탄올 추출물과 열수 추출물이 $1,000{\mu}g/ml$에서 각각 96.6%, 60.0%의 활성을 나타내었다. 청미래덩굴의 에탄올 추출물의 세포생존율을 확인하기 위하여 섬유아세포(CCD-986sk)로 MTT assay에 의해 확인한 결과 $50{\mu}g/ml$ 농도에서 71.7%의 세포 생존율을 확인할 수 있었다. 섬유아 세포에서 청미래덩굴 에탄올 추출물의 pro-collagen type-1 생합성량을 확인한 결과 $50{\mu}g/ml$의 농도에서 139% 이상의 우수한 효과를 나타내었으며, MMP-1 저해활성을 측정한 결과 $50{\mu}g/ml$의 각각의 농도에서 74.9%의 활성을 나타내었다. 청미래덩굴 에탄올 추출물을 처리한 섬유아 세포에서 MMP family 중 MMP-1의 단백질 발현 및 mRNA 발현 억제 효과를 확인한 결과 MMP-1 단백질 발현과 mRNA 발현 모두 $50{\mu}g/ml$에서 각각 35%, 45%의 저해율을 나타내었다. 이러한 결과로 미루어 보아 청미래덩굴 에탄올 추출물이 주름개선용 화장품 소재로 응용이 가능할 것으로 판단된다.

The purpose of this study was to research the biological activity of ethanol extract from Smilax china L. which is a vine shrub belonging to the lily family. For antiwrinkle effects, elastase inhibition effect of ethanol and water extracts from S. china L. showed 41.1% and 16.3% at $1,000{\mu}g/ml$ concentration. The collagenase inhibition effect of ethanol and water extracts from S. china L. showed more than 96.6% and 60.0% at $1,000{\mu}g/ml$ concentration. As a result of having fibroblast measured cell viability on fibroblast cell of ethanol extract from S. china L., it showed 71.7% with cell viability at $100{\mu}g/ml$ concentration. At $50{\mu}g/ml$ concentration, the procollagen biosynthesis effect of ethanol extract from S. china L. was 139.86%. At the same concentration, the matrix metalloprotease (MMP)-1 inhibition effect of the ethanol extract was 74.9%. According to the results of Western blot of ethanol extract from S. china L., the expression of the MMP-1 protein was decreased by 35% at $50{\mu}g/ml$ concentration. Reverse transcription-polymerase chain reaction (PCR) of ethanol extract from S. china L. showed that the expression of MMP-1 mRNA was decreased by 45% at $50{\mu}g/ml$ concentration. The findings suggest that 70% ethanol extract from S. china L. (SC) has great potential as a cosmeceutical ingredient with antiwrinkle effects.

키워드

참고문헌

  1. Cannell, R. J., Kellan, S. J., Owsianks, A. M. and Walker, J. M. 1988. Results of a large scale screen of microalgae for the production of protease inhibitors. Planta Med 54, 10-14. https://doi.org/10.1055/s-2006-962319
  2. Carmichael, J., DeGraff, W. G., Gazdar, A. F., Minna, J. D. and Mitchell, J. B. 1987. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res 47, 936-942.
  3. El-Domyati, M., Attia, S., Saleh, F., Brown, D., Birk, D. E., Gasparro, F., Ahmad, H. and Uitto, J. 2002. Intrinsic aging vs. photoaging: a comparative histopathological, immunohistochemical, and ultrastructural study of skin. Exp Dermatol 11, 398-405. https://doi.org/10.1034/j.1600-0625.2002.110502.x
  4. Giacomoni, P. U. and Rein, G. 2001. Factors of skin ageing share common mechanisms. Biogerontology 2, 219-229. https://doi.org/10.1023/A:1013222629919
  5. Gross, J. and Lapiere, C. M. 1962. Collagenolytic activity in amphibian tissues: a tissue culture assay. Proc Natl Acad Sci USA 54, 1197-1204.
  6. Jeroma, S. P., Gabrielle, L. and Raul, F. 1998. Identification of collagen fibrils in scleroderma skin. J Invest Dermatol 90, 48-54.
  7. Kim, D. H. 2011. The study on verification of skin pharmacological mechanism from useful compound of Aruncus dioicus and special cosmetic formular. Ph. D. dissertation, Daegu Haany University, Gyeongbuk, Korea.
  8. Kondo, S. 2000. The roles of cytokines in photoaging. J Dermatol Sci 23, 30-36. https://doi.org/10.1016/S0923-1811(99)00076-6
  9. Lee, J. Y., Park, W. S., Son, E. D., Ahn, S. M. and Hwang, J. S. 2007. In vitro evalution methods for the development of cosmeceutical ingredients. J Alternat Animal Exp 1, 33-40.
  10. Lim, A. K., Kim, J. O., Jung, M. J., Jung, H. K., Hong, J. H. and Kim, D. I. 2008. Functional biological activity of hot water and ethanol extracts from Taraxaci Herba. J Korean Soc Food Sci Nutr 37, 1231-1237. https://doi.org/10.3746/jkfn.2008.37.10.1231
  11. Nagase, H. and Woessner, J. F. Jr. 1999. Matrix metalloproteinase. J Biol Chem 274, 21491-21494. https://doi.org/10.1074/jbc.274.31.21491
  12. Seo, I. A. 2010. Studies on cosmeceutical of Phaseolus radiatus. M. S. dissertation, Daegu Haany University, Gyeongbuk, Korea.
  13. Shao, B., Guo, H., Cui, Y., Ye, M., Han, J. and Guo, D. 2007. Steroidal saponins from Smilax china and their anti-inflammatory activities. Phytochemistry 68, 623-630. https://doi.org/10.1016/j.phytochem.2006.10.026
  14. Song, H. S., Park, Y. H., Jung, S. H., Kim, D. P., Jung, Y. H., Lee, M. K. and Moon, K. Y. 2006. Antioxidant activity of extracts from Smilax china Root. J Korean Soc Food Sci Nutr 35, 1133-1138. https://doi.org/10.3746/jkfn.2006.35.9.1133
  15. Talwar, H. S., Griffiths, C. E., Fisher, G. J., Hamilton, T. A. and Voorhees, J. J. 1995. Reduced type I and type III procollagens in photodamaged adult human skin. J Invenst Dermatol 105, 285-290. https://doi.org/10.1111/1523-1747.ep12318471
  16. Wang, Y., Johnson, A. R., Ye, Q. Z. and Dyer, R. D. 1999. Catalytic activities and substrate specificity of the human membrane type 4 matrix metalloproteinase catalytic domain. J Biol Chem 274, 33043-33049. https://doi.org/10.1074/jbc.274.46.33043
  17. Wlaschek, M., Tantcheva-Poor, I., Naderi, L., Ma, W., Schneider, L. A., Razi-Wolf, Z., Schuller, J. and Scharffetter-Kochanek, K. 2001. Solar UV irradiation and dermal photoaging. J Photochem Photobiol B 63, 41-51. https://doi.org/10.1016/S1011-1344(01)00201-9
  18. Wu, L. S., Wang, X. J., Wang, H., Yang, H. W., Jia, A. Q. and Ding, Q. 2010. Cytotoxic polyphenols against breast tumor cell in Smilax china L. J Ethnopharmacol 130, 460-464. https://doi.org/10.1016/j.jep.2010.05.032
  19. Wunsch, E. and Heindrich, H. G. Zur. 1963. Quantitativen bestimmung der collagenase. Hoppe-Seyler's Physiol Chem 333, 149-151. https://doi.org/10.1515/bchm2.1963.333.1.149
  20. Yang, K. H. 2004. Studies on the antioxidation and antimicrobial effect of smilacis glabrae rhizome. J Korean Oriental Med 25, 1-8.

피인용 문헌

  1. Effect of Anti-Skin Wrinkle and Antioxidant of Agastache rugosa Kentz through Fermentation Process of the lactic acid vol.23, pp.1, 2015, https://doi.org/10.7783/KJMCS.2015.23.1.37
  2. Mechanisms for Anti-wrinkle Activities from Fractions of Black Chokeberries vol.26, pp.1, 2016, https://doi.org/10.5352/JLS.2016.26.1.34
  3. on CCD-986sk vol.60, pp.4, 2017, https://doi.org/10.3839/jabc.2017.056