The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Hydrogen Peroxide- and Nitric Oxide-mediated Disease Control of Bacterial Wilt in Tomato Plants

  • Hong, Jeum Kyu (Department of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech)) ;
  • Kang, Su Ran (Department of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech)) ;
  • Kim, Yeon Hwa (Department of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech)) ;
  • Yoon, Dong June (Department of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech)) ;
  • Kim, Do Hoon (Department of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech)) ;
  • Kim, Hyeon Ji (Department of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech)) ;
  • Sung, Chang Hyun (Department of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech)) ;
  • Kang, Han Sol (Department of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech)) ;
  • Choi, Chang Won (Department of Biology and Medical Science, Paichai University) ;
  • Kim, Seong Hwan (Department of Microbiology and Institute of Basic Sciences, Dankook University) ;
  • Kim, Young Shik (Department of Plant Science and Food Biotechnology, Sangmyung University)
  • Received : 2013.04.23
  • Accepted : 2013.08.23
  • Published : 2013.12.01
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Abstract

Reactive oxygen species (ROS) generation in tomato plants by Ralstonia solanacearum infection and the role of hydrogen peroxide ($H_2O_2$) and nitric oxide in tomato bacterial wilt control were demonstrated. During disease development of tomato bacterial wilt, accumulation of superoxide anion ($O_2{^-}$) and $H_2O_2$ was observed and lipid peroxidation also occurred in the tomato leaf tissues. High doses of $H_2O_2$ and sodium nitroprusside (SNP) nitric oxide donor showed phytotoxicity to detached tomato leaves 1 day after petiole feeding showing reduced fresh weight. Both $H_2O_2$ and SNP have in vitro antibacterial activities against R. solanacearum in a dose-dependent manner, as well as plant protection in detached tomato leaves against bacterial wilt by $10^6$ and $10^7$ cfu/ml of R. solanacearum. $H_2O_2$- and SNP-mediated protection was also evaluated in pots using soil-drench treatment with the bacterial inoculation, and relative 'area under the disease progressive curve (AUDPC)' was calculated to compare disease protection by $H_2O_2$ and/or SNP with untreated control. Neither $H_2O_2$ nor SNP protect the tomato seedlings from the bacterial wilt, but $H_2O_2$ + SNP mixture significantly decreased disease severity with reduced relative AUDPC. These results suggest that $H_2O_2$ and SNP could be used together to control bacterial wilt in tomato plants as bactericidal agents.

Keywords

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