한국동물위생학회지 (Korean Journal of Veterinary Service)

  • 제37권4호
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  • Pages.247-252
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  • 2014
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  • 3022-7372(pISSN)
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  • 2287-7630(eISSN)
한국동물위생학회 (The Korean Society of Veterinary Service)
권호 표지
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Mycoplasma hyopneumoniae와 Mycoplasma hyorhinis 동시 감별진단을 위한 다중진단 중합효소반응

Simultaneous diagnosis and differentiation of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis infections by multiplex PCR

  • 홍선화 (원광대학교 동물자원개발연구센터) ;
  • 이현아 (원광대학교 동물자원개발연구센터) ;
  • 김동우 (원광대학교 동물자원개발연구센터) ;
  • 김태완 (경북대학교 수의과대학) ;
  • 김옥진 (원광대학교 동물자원개발연구센터)
  • Hong, Sunhwa (Center for Animal Resource Development, Wonkwang University) ;
  • Lee, Hyun-A (Center for Animal Resource Development, Wonkwang University) ;
  • Kim, Dong-Woo (Center for Animal Resource Development, Wonkwang University) ;
  • Kim, Tae-Wan (College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Okjin (Center for Animal Resource Development, Wonkwang University)
  • 투고 : 2014.08.11
  • 심사 : 2014.11.05
  • 발행 : 2014.12.30
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초록

The economic impact of swine mycoplasma infection is high. An accurate diagnosis is often difficult and time consuming. We report the development and validation of an effective multiplex polymerase chain reaction (PCR) assay that detects Mycoplasma (M.) hyopneumoniae and M. hyorhinis. The multi detection of M. hyopneumoniae and M. hyorhinis primer set were employed to detect mycoplasma species and typing of the species was performed on the basis of sequence analysis of the PCR product. The target nucleic acid fragments were specifically amplified by M. hyopneumoniae and M. hyorhinis PCR with 16S ribosomal DNA primers. Single and mixed Mycoplasma species DNA templates were used to evaluate the specificity of the multiplex assay. The corresponding specific DNA products were amplified for each pathogen. The multiplex PCR assay provides a novel tool for simultaneous detection and differentiation of M. hyopneumoniae and M. hyorhinis.

키워드

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피인용 문헌

  1. Age-specific Prevalence of Porcine Reproductive and Respiratory Syndrome Virus, Porcine Circovirus Type 2, and Mycoplasma hyopneumoniae in Korea Pig Farms vol.50, pp.1, 2014, https://doi.org/10.4167/jbv.2020.50.1.009