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국내산 유채 종자의 품종별 지방 조성 및 페놀 추출물의 항산화 활성

Lipid Composition of Korean Rapeseed (Brassica napus L.) Cultivar and Antioxidant Capacity of Phenolic Extract

  • Lee, A-Young (Department of Food and Nutrition, Daegu University) ;
  • Hong, Soon-Taek (Department of Food Science and Technology, Chungnam National University) ;
  • Jang, Young-Seok (National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Jeung-Hee (Department of Food and Nutrition, Daegu University)
  • 투고 : 2014.09.05
  • 심사 : 2014.11.03
  • 발행 : 2014.12.31

초록

본 연구는 국내산 유채 종자 내한, 탐라, 목포111호, 영산, 탐미, 한라와 목포68호의 품종별 지방산 조성, 인지질, 당지질, 토코페롤 함량을 조사하고, 유채 종자를 탈지한 후 crude phenolic compound를 추출하여 총 페놀 함량과 항산화 활성을 분석하였다. 유채 종자의 조지방 함량은 29.3~33.2%였고, 품종들 간의 유의적인 차이는 없는 것으로 나타났다. 주요 지방산은 oleic(n-9), linoleic(n-6)과 ${\alpha}$-linolenic acid(n-3)였으며, n-6/n-3 지방산의 비율은 2.20~3.68이고 이 중 내한종이 가장 낮고 한라종이 가장 높았다. Neutral lipids 함량은 내한종과 탐라종, 인지질 함량은 탐미종에서 가장 높고, 소량 함유된 당지질은 내한, 탐라, 한라와 목포68호 간 함량 차이가 없었으며 목포111호에 가장 낮게 함유되었다. 토코페놀 함량 수준은 ${\gamma}$ > ${\alpha}$ > ${\beta}$ > ${\delta}$-tocopherol이며, 내한종의 총 tocopherol 함량이 가장 높고 다음으로 탐라, 목포111의 순이었으며 영산, 탐미, 한라 목포68호는 유의적인 함량 차이를 보이지 않았다. 품종별 총 페놀 함량은 탐라> 영산> 탐미, 한라, 내한> 목포68호, 목포111호의 순으로 나타났으며, 탐라종의 DPPH 라디칼 소거능과 FRAP value가 가장 높아 7개의 품종 중 항산화 활성이 가장 높은 것으로 확인되었다. 탈지 후의 유채박에 함유된 phenol compounds의 용매별 분획할 결과 30% ethanol fraction에 가장 많이 추출되며 이 fraction의 항산화 활성이 가장 높게 나타났다.

This study investigated lipid profiles and antioxidant capacities of seven Korean rapeseed cultivars (Naehan, Tamla, Mokpo111, Yeongsan, Tammi, Hanla, and Mokpo68). The rapeseeds contained 29.3~33.2% of extracted lipid and major fatty acids were oleic, linoleic, and linolenic acids. The ratio of omega-6/omega-3 fatty acids was 2.20~3.68 with the highest in Hanla and lowest in Naehan. Glycolipid ranged from 0.21 g/100 g to 0.47 g/100 g. Phospholipid content was 0.55~1.15 g/100 g with the highest in Tammi and the lowest in Mokpo68, and the most common phospholipid was phosphatidylcholine. Tocopherol content was 9.45~15.11 mg/100 g in the order of ${\gamma}$ > ${\alpha}$ > ${\beta}$ > ${\delta}$-tocopherol, and Naehan contained the highest amount of tocopherols (P<0.05). Total phenol content (TPC) of rapeseed was 314.64~577.08 mg SAE/100 g. Tamla contained the highest TPC, and showed the highest antioxidant activity determined by 1,1-diphenyl-2-picrylhydrazyl free radical scavenging capacity and ferric reducing antioxidant power.

키워드

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피인용 문헌

  1. 유채꽃 가공유형별 플라보노이드 조성 및 함량 변화 vol.36, pp.1, 2014, https://doi.org/10.5338/kjea.2017.36.1.04
  2. Antioxidative capacity of hydrolyzed rapeseed cake extract and oxidative stability of fish oil-in-water emulsion added with the extract vol.24, pp.4, 2014, https://doi.org/10.11002/kjfp.2017.24.4.529