Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Comparison of Radiation-Induced Hydrocarbons in Dried Anchovies by Electron-Beam Irradiation

전자선 조사한 건조 멸치류에서 유도된 지방분해산물의 분석

  • Received : 2014.11.03
  • Accepted : 2014.12.02
  • Published : 2014.12.31
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Abstract

Radiation-induced hydrocarbon contents of dried anchovy, jiri anchovy, and large-eyed herring were evaluated following electron-beam irradiation at doses of 1, 3, 5, 7, and 10 kGy. GC/MS identification of the induced hydrocarbons by irradiation was conducted after lipid separation by soxtec, followed by florosil column chromatography. 1-Tetradecene ($C_{14:1}$) and pentadecane ($C_{15:0}$) derived from palmitic acid, 1-hexadecene ($C_{16:1}$) and heptadecane ($C_{17:0}$) from stearic acid, and 8-heptadecene ($C_{17:1}$) and 1,7-hexadecadiene ($C_{16:2}$) from oleic acid were the major induced hydrocarbons in irradiated dried anchovy, jiri anchovy, and large-eyed herring samples. At the same irradiation dose, concentration of induced hydrocarbons differed from fatty acid composition and increased in accordance with radiation dose level. Radiation-induced hydrocarbons, such as 1-tetradecene ($C_{14:1}$), 1-hexadecene ($C_{16:2}$), 8-heptadecene ($C_{17:1}$), and 1,7-hexadecadiene ($C_{16:2}$), were confirmed as irradiation marker compounds. Therefore, these marker compounds could be used to distinguish electron-beam irradiated dried anchovy, jiri anchovy, and large-eyed herring from non-irradiated ones.

건조된 국멸치와 지리멸치 및 밴댕이에 1, 3, 5, 7 및 10 kGy의 선량으로 전자선을 조사하여 비조사 시료와 조사된 시료에서 지방을 추출한 후 전자선 조사 시 유도 생성되는 hydrocarbon류를 florisil column chromatography를 이용하여 분리하였으며, hydrocarbon류의 함량 및 분해패턴은 GC/MS로 확인하였다. 전자선 조사된 건조 멸치류에서 palmitic acid로부터 생성되는 1-tetradecene($C_{14:1}$), pentadecane($C_{15:0}$)과 stearic acid로부터 1-hexadecene($C_{16:1}$), heptadecane($C_{17:0}$) 및 oleic acid로부터 8-heptadecene($C_{17:1}$), 1,7-hexadecadiene($C_{16:2}$)이 확인되었으며, 6종의 hydrocarbon류는 조사선량에 따라 증가하였다. 건조된 멸치류에서 유기용매에서 기인한 것으로 판단되는 pentadecane($C_{15:0}$) 및 heptadecane($C_{17:0}$)을 제외한 hydrocarbon류 중 8-heptadecene($C_{17:1}$)의 함량이 가장 높았으며, 다음으로 1-tetradecene($C_{14:1}$), 1-hexadecene($C_{16:1}$), 1,7-hexadecadiene($C_{16:2}$) 순으로 확인되었다. 따라서 전자선 조사된 건조 멸치류에서 분석된 hydrocarbon류 중 1-tetradecene($C_{14:1}$), 1-hexadecene($C_{16:1}$) 그리고 8-heptadecene($C_{17:1}$) 및 1,7-hexadecadiene($C_{16:2}$)은 전자선 조사 여부를 판별할 수 있는 유용한 지표로 활용될 수 있을 것이라고 사료된다.

Keywords

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