한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제22권6호
  • /
  • Pages.457-462
  • /
  • 2014
  • /
  • 1225-9306(pISSN)
  • /
  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
권호 표지
DOI QR코드

DOI QR Code

피부 각질세포에 대한 비파엽 에탄올 추출물의 PGE2 조절 작용과 상처치료 효과

Prostaglandin E2 Up-regulation and Wound Healing Effect of the Ethanol Extract of Eriobotryae Folium in Human Keratinocyte

  • 임도연 (광주여자대학교 교양교직과정부) ;
  • 이경인 (동신대학교 생물자원산업화지원센터)
  • Im, Do Youn (Division of Liberal Arts and Teacher Training, Kwangju Women's University) ;
  • Lee, Kyoung In (Biotechnology Industrialization Center, Dongshin University)
  • 투고 : 2014.09.22
  • 심사 : 2014.11.16
  • 발행 : 2014.12.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Prostaglandin (PG) $E_2$ is an important mediator of skin wound healing without excessive scarring and gastric ulcer healing. However, $PGE_2$ has a short lifetime in vivo because it is metabolized rapidly by 15-hydroxyprostaglandin dehydrogenase (15-PGDH). Ethanol extract of Eriobotryae folium (EFEE) elevated intracellular and extracellular $PGE_2$ levels in HaCaT cells and inhibited 15-PGDH ($ED_{50}$ : $168.4{\mu}g/mL$) with relatively low cytotoxicity ($IC_{50}$ : $250.0{\mu}g/mL$). Real-time PCR analysis showed that mRNA expression of cyclooxygenase (COX)-1 and COX-2 enzymes were increased and prostaglandin transporter (PGT) was decreased in HaCaT cells by EFEE. Moreover, wound healing effect of EFEE ($168.4{\mu}g/mL$) was comparable to that of TGF-${\beta}1$ (300 pg/mL) as a positive control. These results demonstrate that EFEE may be valuable therapeutic materials for the treatment of $PGE_2$ level dependent diseases.

키워드

참고문헌

  1. Bae DH, You YH, Yoon HG, Kim KM, Lee YH, Kim YJ, Baek HY, Kim SO, Lee JM and Jun WJ. (2010). Protective effects of loquat(Eriobotrya japonica) leaves against ethanol-induced toxicity in HepG2 cells transfected with CYP2E1. Food Science and Biotechnology. 19:1093-1096. https://doi.org/10.1007/s10068-010-0154-3
  2. Banno N, Akihisa T, Tokuda H, Yasukawa K, Taguchi Y, Akazawa H, Ukiya M, Kimura Y, Suzuki T and Nishino H. (2005). Anti-inflammatory and antitumor-promoting effects of the triterpene acids from the leaves of Eriobotrya japonica. Biological and Pharmaceutical Bulletin. 28:1995-1999. https://doi.org/10.1248/bpb.28.1995
  3. Cha DS, Eun JS and Jeon H. (2011a). Anti-inflammatory and antinociceptive properties of the leaves of Eriobotrya japonica. Journal of Ethnopharmacology. 134:305-312. https://doi.org/10.1016/j.jep.2010.12.017
  4. Cha DS, Shin TY, Eun JS, Kim DK and Jeon H. (2011b). Antimetastatic properties of the leaves of Eriobotyra japonica. Archives of Pharmacal Research. 34:425-436. https://doi.org/10.1007/s12272-011-0310-1
  5. Choi YG, Seok YH, Yeo SJ, Jeong MY and Lim S. (2011). Protective changes of inflammation-related gene expression by the leaves of Eriobotyra japonica in the LPS-stimulated human gingival fibroblast: Microarray analysis. Journal of Ethnopharmacology. 135:636-645. https://doi.org/10.1016/j.jep.2011.03.066
  6. Chatterjee A, Chatterjee S, Das S, Saha A, Chattopadhyay S and Bandyopadhyay SK. (2012). Ellagic acid facilitates indomethacin-induced gastric ulcer healing via COX-2 upregulation. Acta Biochimica et Biophysica Sinica. 44:565-576. https://doi.org/10.1093/abbs/gms034
  7. Choi DB, Piao YL, Wu Y and Cho H. (2013). Control of the intracellular levels of prostaglandin $E_2$ through inhibition of the 15-hydroxyprostaglandin dehydrogenase for wound healing. Bioorganic and Medicinal Chemistry. 21:4477-4484. https://doi.org/10.1016/j.bmc.2013.05.049
  8. Ensor CM, Yang JY, Okita RT and Tai HH. (1990). Cloning and sequencing analysis of the cDNA for human placental $NAD^+$-dependent15-hydroxyprostaglandin dehydrogenase. Journal of Biological Chemistry. 265:14888-14891.
  9. de-Faria FM, Almeida ACA, Luiz-Ferreira A, Dunder RJ, Takayama C, Silva MS, Silva MA, Vilegas W, Rozza AL, Pellizzon CH, Toma W and Souza-Brito ARM. (2012). Mechanisms of action underlying the gastric antiulcer activity of the Rhizophora mangle L. Journal of Ethnopharmacology. 139:234-243. https://doi.org/10.1016/j.jep.2011.11.007
  10. Fujimoto Y, Sakuma S, Komatsu S, Sato D, Nishida H, Xiao YQ, Bada K and Fujita T. (1998). Inhibition of 15-hydroxyprostaglandin dehydrogenase activity in rabbit gastric antral mucosa by panaxynol isolated from oriental medicines. Journal of Pharmacy and Pharmacology. 50:1075-1078. https://doi.org/10.1111/j.2042-7158.1998.tb06925.x
  11. Hintermann E, Bilban M, Sharabi A and Quaranta V. (2001). Inhibitory role of alpha 6 beta 4-associated erbB-2 and phosphoinositide 3-kinase in keratinocyte haptotactic migration dependent on alpha 3 beta 1 integrin. Journal of Cell Biology. 153:465-478. https://doi.org/10.1083/jcb.153.3.465
  12. Iijima Y, Kawakita N and Yamazaki M. (1980). Inhibition of 15-hydroxyprostaglandin dehydroxygenase by antiallegic agents. Biochemical and Biophysical Research Communications. 93:912-918. https://doi.org/10.1016/0006-291X(80)91162-6
  13. Ito H, Kobayashi E, Takamatsu Y, Li SH, Hatano T, Sakagami H, Kusama K, Satoh K, Suqita D, Shimura S, Itoh Y and Yoshida T. (2000). Polyphenols from Eriobotrya japonica and their cytotoxicity against human oral tumor cell lines. Chemical and Pharmaceutical Bulletin. 48:687-693. https://doi.org/10.1248/cpb.48.687
  14. Koivisto L, Jiang G, Häkkinen L, Chan B and Larjava H. (2006). HaCaT keratinocyte migration is dependent on epidermal growth factor receptor signaling and glycogen synthase kinase-3alpha. Experimental Cell Research. 312:2791-2805. https://doi.org/10.1016/j.yexcr.2006.05.009
  15. Kolodsick JE, Peters-Golden M, Larios J, Toews GB, Thannickal VJ and Moore BB. (2003). Prostaglandin E2 inhibits fibroblast to myofibroblast transition via E. prostanoid receptor 2 signaling and cyclic adenosine monophosphate elevation. American Journal of Respiratory Cell and Molecular Biology. 29:537-544. https://doi.org/10.1165/rcmb.2002-0243OC
  16. Lee KI, Moon YS, Pyo BS and Choi CH. (2012). Extracellular prostaglandin $E_2$ upregulation effect of the methanol extract of Artemisia argyi. Natural Product Science. 18:211-214.
  17. Li YJ, Wang XQ, Sato T, Kanaji N, Nakanishi M, Kim M, Michalski J, Nelson AJ, Sun JH, Farid M, Basma H, Patil A, Toews ML, Liu X and Rennard SI. (2011). Prostaglandin $E_2$ inhibits human lung fibroblast chemotaxis through disparate actions on different E-prostanoidreceptors. American Journal of Respiratory Cell and Molecular Biology. 44:99-107. https://doi.org/10.1165/rcmb.2009-0163OC
  18. Liu Y, Dulchavsky DS, Gao X, Kwon D, Chopp M, Dulchavsky S and Gautam SC. (2006). Wound repair by bone marrow stromal cells through growth factor production. Journal of Surgical Research. 136:336-341. https://doi.org/10.1016/j.jss.2006.07.037
  19. Moustafa TM, El-Azab MF and Fouda A. (2013). 15-PGDH inhibitors: The antiulcer effects of carbenoxolone, pioglitazone and verapamil in indomethacin induced peptic ulcer rats. European Review for Medical and Pharmacological Sciences. 17:2000-2009.
  20. Savla U, Appel HJ, Sporn PH and Waters CM. (2001). Prostaglandin E(2) regulates wound closure in airway epithelium. Lung Cellular and Molecular Physiology. 280:L421-431. https://doi.org/10.1152/ajplung.2001.280.3.L421
  21. Sandulache VC, Parekh A, Li-Korotky H, Dohar JE and Hebda PA. (2007). Prostaglandin $E_2$ inhibition of keloid fibroblast migration, contraction, and transforming growth factor(TGF)-${\beta}1$-induced collagen synthesis. Wound Repair and Regeneration. 15:122-133. https://doi.org/10.1111/j.1524-475X.2006.00193.x
  22. Schuster VL. (1998). Molecular mechanisms of prostaglandin transport. Annual Review of Physiology. 60:221-242. https://doi.org/10.1146/annurev.physiol.60.1.221
  23. Schuster VL. (2002). Prostaglandin transport. Prostaglandins and Other Lipid Mediators. 68-69:633-647. https://doi.org/10.1016/S0090-6980(02)00061-8
  24. Strutz F, Zeisberg M, Renziehausen A, Raschke B, Becker V, van Kooten C and Muller G. (2001). TGF-beta 1 induces proliferation in human renal fibroblasts via induction of basic fibroblast growth factor(FGF-2). Kidney International. 59:579-592. https://doi.org/10.1046/j.1523-1755.2001.059002579.x
  25. Wilgus TA, Bergdall VK, Tober KL, Hill KJ, Mitra S, Flavahan NA and Oberyszyn TM. (2004). The impact of cyclooxygenase-2 mediated inflammation on scarless fetal wound healing. American Journal of Pathology. 165:753-761. https://doi.org/10.1016/S0002-9440(10)63338-X
  26. Yamamoto S, Watabe K, Araki H, Kamada Y, Kato M, Kizu T, Kiso S, Tsutsui S, Tsujii M, Kihara S, Funahashi T, Shimomura I, Hayashi N and Takehara T. (2012). Protective role of adiponectin against ethanol-induced gastric injury in mice. Gastrointestinal and Liver Physiology. 302:G773-G780. https://doi.org/10.1152/ajpgi.00324.2011
  27. Uto T, Suangkaew N and Morinaga O. (2010). Eriobotryae folium extract suppresses LPS-induced iNOS and COX-2 expression by inhibition of NF-kappaB and MAPK activation in murine macrophages. American Journal of Chinese Medicine. 38:985-994. https://doi.org/10.1142/S0192415X10008408
  28. Yang Y, Huang Y, Huang C, Lv X, Liu L, Wang Y and Li J. (2012). Antifibrosis effects of triterpene acids of Eriobotrya japonica(Thunb.) Lindl. leaf in a rat model of bleomycininduced pulmonary fibrosis. Journal of Pharmacy and Pharmacology. 64:1751-1760. https://doi.org/10.1111/j.2042-7158.2012.01550.x
  29. Zhou H, Felsen D, Sandulache VC, Amin MR, Kraus DH and Branski RC. (2012). Prostaglandin(PG) $E_2$ exhibits anti-fibrotic activity in vocal fold fibroblasts. Laryngoscope. 121:1261-1265.