The Journal of Advanced Prosthodontics

The Korean Academy of Prosthodonitics (대한치과보철학회)
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The influence of systemically administered oxytocin on the implant-bone interface area: an experimental study in the rabbit

  • Cho, Sung-Am (Department of Prosthodontics, School of Dentistry, Kyoungpook National University) ;
  • Park, Sang-Hun (Department of Prosthodontics, School of Dentistry, Kyoungpook National University) ;
  • Cho, Jin-Hyun (Department of Prosthodontics, School of Dentistry, Kyoungpook National University)
  • Received : 2014.03.26
  • Accepted : 2014.08.05
  • Published : 2014.12.31
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Abstract

PURPOSE. The purpose of this study was to assess the effect of systemically administered oxytocin (OT) on the implant-bone interface by using histomorphometric analysis and the removal torque test. MATERIALS AND METHODS. A total of 10 adult, New Zealand white, female rabbits were used in this experiment. We placed 2 implants (CSM; CSM Implant, Daegu, South Korea) in each distal femoral metaphysis on both the right and left sides; the implants on both sides were placed 10 mm apart. In each rabbit, 1 implant was prepared for histomorphometric analysis and the other 3 were prepared for the removal torque test (RT). The animals received intramuscular injections of either saline (control group; 0.15 M NaCl) or OT (experimental group; $200{\mu}g/rabbit$). The injections were initiated on Day 3 following the implant surgery and were continued for 4 subsequent weeks; the injections were administered twice per day (at a 12-h interval), for 2 days per week. RESULTS. While no statistically significant difference was observed between the two groups (P=.787), the control group had stronger removal torque values. The serum OT concentration (ELISA value) was higher in the OT-treated group, although no statistically significant difference was found. Further, the histomorphometric parameter (bone-to-implant contact [BIC], inter-thread bone, and peri-implant bone) values were higher in the experimental group, but the differences were not significant. CONCLUSION. We postulate that OT supplementation via intramuscular injection weakly contributes to the bone response at the implant-bone interface in rabbits. Therefore, higher concentrations or more frequent administration of OT may be required for a greater bone response to the implant. Further studies analyzing these aspects are needed.

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References

  1. Adell R, Lekholm U, Rockler B, Branemark PI. A 15-year study of osseointegrated implants in the treatment of the edentulous jaw. Int J Oral Surg 1981;10:387-416. https://doi.org/10.1016/S0300-9785(81)80077-4
  2. Cochran DL, Morton D, Weber HP. Consensus statements and recommended clinical procedures regarding loading protocols for endosseous dental implants. Int J Oral Maxillofac Implants 2004;19:109-13.
  3. Le Guehennec L, Soueidan A, Layrolle P, Amouriq Y. Surface treatments of titanium dental implants for rapid osseointegration. Dent Mater 2007;23:844-54. https://doi.org/10.1016/j.dental.2006.06.025
  4. Sykaras N, Woody RD, Lacopino AM, Triplett RG, Nunn ME. Osseointegration of dental implants complexed with rhBMP-2: a comparative histomorphometric and radiographic evaluation. Int J Oral Maxillofac Implants 2004;19:667-78.
  5. Blom EJ, Verheij JG, de Blieck-Hogervorst JM, Di Silvio L, Klein CP. Cortical bone ingrowth in growth hormone-loaded grooved implants with calcium phosphate coatings in goat femurs. Biomaterials 1998;19:263-70. https://doi.org/10.1016/S0142-9612(98)00205-1
  6. Stenport VF, Olsson B, Morberg P, Tornell J, Johansson CB. Systemically administered human growth hormone improves initial implant stability: an experimental study in the rabbit. Clin Implant Dent Relat Res 2001;3:135-41. https://doi.org/10.1111/j.1708-8208.2001.tb00133.x
  7. Tresguerres IF, Clemente C, Donado M, Gomez-Pellico L, Blanco L, Alobera MA, Tresguerres JA. Local administration of growth hormone enhances periimplant bone reaction in an osteoporotic rabbit model. Clin Oral Implants Res 2002;13:631-6. https://doi.org/10.1034/j.1600-0501.2002.130609.x
  8. Park JW, Kim JM, Lee HJ, Jeong SH, Suh JY, Hanawa T. Bone healing with oxytocin-loaded microporous ${\beta}$-TCP bone substitute in ectopic bone formation model and critical-sized osseous defect of rat. J Clin Periodontol 2014;41:181-90. https://doi.org/10.1111/jcpe.12198
  9. Tamma R, Colaianni G, Zhu LL, DiBenedetto A, Greco G, Montemurro G, Patano N, Strippoli M, Vergari R, Mancini L, Colucci S, Grano M, Faccio R, Liu X, Li J, Usmani S, Bachar M, Bab I, Nishimori K, Young LJ, Buettner C, Iqbal J, Sun L, Zaidi M, Zallone A. Oxytocin is an anabolic bone hormone. Proc Natl Acad Sci USA 2009;106:7149-54. https://doi.org/10.1073/pnas.0901890106
  10. Colaianni G, Di Benedetto A, Zhu LL, Tamma R, Li J, Greco G, Peng Y, Dell Endice S, Zhu G, Cuscito C, Grano M, Colucci S, Iqbal J, Yuen T, Sun L, Zaidi M, Zallone A. Regulated production of the pituitary hormone oxytocin from murine and human osteoblasts. Biochem Biophys Res Commun 2011;411:512-5. https://doi.org/10.1016/j.bbrc.2011.06.158
  11. Colli VC, Okamoto R, Spritzer PM, Dornelles RC. Oxytocin promotes bone formation during the alveolar healing process in old acyclic female rats. Arch Oral Biol 2012;57:1290-7. https://doi.org/10.1016/j.archoralbio.2012.03.011
  12. Amar AP, Weiss MH. Pituitary anatomy and physiology. Neurosurg Clin N Am 2003;14:11-23. https://doi.org/10.1016/S1042-3680(02)00017-7
  13. Lee HJ, Macbeth AH, Pagani JH, Young WS 3rd. Oxytocin: the great facilitator of life. Prog Neurobiol 2009;88:127-51.
  14. Colaianni G, Tamma R, Di Benedetto A, Yuen T, Sun L, Zaidi M, Zallone A. The oxytocin-bone axis. J Neuroendocrinol 2014;26:53-7. https://doi.org/10.1111/jne.12120
  15. Breuil V, Amri EZ, Panaia-Ferrari P, Testa J, Elabd C, Albert-Sabonnadiere C, Roux CH, Ailhaud G, Dani C, Carle GF, Euller-Ziegler L. Oxytocin and bone remodelling: relationships with neuropituitary hormones, bone status and body composition. Joint Bone Spine 2011;78:611-5. https://doi.org/10.1016/j.jbspin.2011.02.002
  16. Petersson M, Lagumdzija A, Stark A, Bucht E. Oxytocin stimulates proliferation of human osteoblast-like cells. Peptides 2002;23:1121-6. https://doi.org/10.1016/S0196-9781(02)00041-4
  17. Luo T, Zhang W, Shi B, Cheng X, Zhang Y. Enhanced bone regeneration around dental implant with bone morphogenetic protein 2 gene and vascular endothelial growth factor protein delivery. Clin Oral Implants Res 2012;23:467-73. https://doi.org/10.1111/j.1600-0501.2011.02164.x
  18. Gomez-Moreno G, Cutando A, Arana C, Worf CV, Guardia J, Munoz F, Lopez-Pena M, Stephenson J. The effects of growth hormone on the initial bone formation around implants. Int J Oral Maxillofac Implants 2009;24:1068-73.
  19. Liu X, Shimono K, Zhu LL, Li J, Peng Y, Imam A, Iqbal J, Moonga S, Colaianni G, Su C, Lu Z, Iwamoto M, Pacifici M, Zallone A, Sun L, Zaidi M. Oxytocin deficiency impairs maternal skeletal remodeling. Biochem Biophys Res Commun 2009;388:161-6. https://doi.org/10.1016/j.bbrc.2009.07.148
  20. MacDonald E, Dadds MR, Brennan JL, Williams K, Levy F, Cauchi AJ. A review of safety, side-effects and subjective reactions to intranasal oxytocin in human research. Psychoneuroendocrinol 2011;36:1114-26. https://doi.org/10.1016/j.psyneuen.2011.02.015
  21. Iseri SO, Sener G, Saglam B, Gedik N, Ercan F, Yegen BC. Oxytocin protects against sepsis-induced multiple organ damage: role of neutrophils. J Surg Res 2005;126:73-81. https://doi.org/10.1016/j.jss.2005.01.021
  22. Petersson M, Lagumdzija A, Stark A, Bucht E. Oxytocin stimulates proliferation of human osteoblast-like cells. Pept 2002;23:1121-6. https://doi.org/10.1016/S0196-9781(02)00041-4
  23. Ryden G, Sjoholm I. Half-life of oxytocin in blood of pregnant and non-pregnant women. Acta Endocrinol (Copenh) 1969;61:425-31.

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