Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Ecotoxicological End-points on Intertidal Mud Crab, Macrophthalmus japonicus, following PFOS (Perfluorooctane Sulfonate) Exposure

PFOS (perfluorooctane sulfonate) 노출에 따른 조간대 칠게(Macrophthalmus japonicus)의 생태독성학적 판정점 제시

  • Kim, Won-Seok (Department of Fisheries and Ocean Science, Chonnam National University) ;
  • Park, Kiyun (Department of Fisheries and Ocean Science, Chonnam National University) ;
  • Nikapitiya, Chamilani (Department of Fisheries and Ocean Science, Chonnam National University) ;
  • Kwak, Ihn-Sil (Department of Fisheries and Ocean Science, Chonnam National University)
  • Received : 2014.10.14
  • Accepted : 2014.12.01
  • Published : 2014.12.31
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Abstract

PFOS (perfluorooctane sulfonate) is one of the perflourinated organic compound, which persist as a residual compound in the coastal environments. Intertidal mud crab Macrophthalmus japonicus mainly inhabits in coastal and bay ecosystems in Indo-Pacific region including Korea and reflects to environmental changes. In the present study, M. japonicus were exposed to different concentrations of PFOS and various ecotoxicological end-points such as survival rate, elimination of appendages, changes of the crust and internal organ color changes were investigated. Interestingly, the PFOS exposure showed concentration-dependent decrease of survival rate. High PFOS exposure ($30{\mu}gL^{-1}$) showed a low survival rate of 24% at 168 hours. Further, in comparison with the controls, the rate of elimination of appendages was also considerably increased in a time dependent manner upon PFOS exposure. Notably, with progression of time, an increased exposure to PFOS, test species showed whitening effect in a concentration-dependent manner, whereas the crab crust color was unchanged in the control. In addition, change in internal organs color and their visibility (clarity) observed in PFOS exposed crabs compared to control. Taken together, we suggest, eco-toxicology end-points of M. japonicus exposed to PFOS gave important biological information which could be useful to identify toxic contamination in the marine benthic environments.

PFOS (Perfluorooctane sulfonate) 노출에 따른 Macrophthalmus japonicus의 생태독성학적 판정점을 탐색하고자 생존율, 부속지 탈락 수, 갑각 및 내장 색의 변화를 관찰하였다. 그 결과, PFOS 노출에 따른 생존율의 감소는 농도 의존적 패턴을 보여 상대적 고농도인 PFOS $30{\mu}gL^{-1}$에서 168시간 노출시 24%의 가장 낮은 생존율을 나타내었다. 부속지 탈락은 대조군에 비해 PFOS 노출군에서 탈락빈도가 증가하였다. 또한 대조군에서는 갑각색의 변화가 관찰되지 않았으나, PFOS 노출군에서는 백화현상이 농도의존적 패턴으로 관찰되었다. 내부 장기의 색 선명도도 PFOS 노출군에서 대조군에 비해 변화가 나타났다. 이러한 결과는 해양 저서환경의 모니터링을 위한 생물적 주요한 정보를 제공해 줄 것이다.

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References

  1. Bossi R, J Strand, O Sortkjær and MM Larsen. 2008. Perfluoroalkyl compounds in Danish wastewater treatment plants and aquatic environments. Environ. Int. 34:443-450. https://doi.org/10.1016/j.envint.2007.10.002
  2. Cho JG, KT Kim, TK Ryu, Y Park, J Yoon, CW Lee, HM Kim, K Choi and KE Jung. 2010. Toxicity of PFCs in embryos of the Oryzias latipes using flow though exposure system. Environ. Health Toxicol. 25:145-151. (in Korean)
  3. Choi YB and SH Jung. 1995. Survey of the waders on the west coast of Korea with special reference to waders on Kwanghwal mudflat in Kimje, Chollabukdo. Kor. J. Ornithol. 2:57-73. (in Korean)
  4. Fang C, Q Huang, Y Ting, Y Chen, L Liu, M Kang, Y Lin, H Shen and S Dong. 2013. Embryonic exposure to PFOS induces immunosuppression in the fish larvae of marine medaka. Ecotox. Environ. Safe. 92:104-111. https://doi.org/10.1016/j.ecoenv.2013.03.005
  5. Giesy JP and K Kannan. 2001. Global distribution of perfluorooctane sulfonate in wildlife. Environ. Sci. Technol. 35:1339-1342. https://doi.org/10.1021/es001834k
  6. Higgins CP, JA Field, CS Criddle and RG Luthy. 2005. Quantitative determination of perfluorochemicals in sediments and domestic sludge. Environ. Sci. Technol. 39:3946-3956. https://doi.org/10.1021/es048245p
  7. Hong SY, KY Park, CW Park, CH Han, HL Suh, SG Yun, CB Song, SG Jo, HS Lim, YS Kang, DJ Kim, CW Ma, MH Son, HK Cha, KB Kim, SD Choi, KY Park, CW Oh, DN Kim, HS Shon, JN Kim, JH Choi, MH Kim and IY Choi. 2006. Marine Invertebrates in Korean coasts. Academy Publishing Company Inc. 1-48.
  8. Horii T, J Kitaura, K Wada and M Nishida. 2001. Genetic relationship among Japanese sentinel crabs. Comp. Biochem. Physiol. B. 130:75-82. https://doi.org/10.1016/S1096-4959(01)00409-2
  9. Inoue K, F Okada, R Ito, S Kato, S Sasaki, S Nakajima, A Uno, Y Saijo, F Sata, Y Yoshimura, R Kishi and H Nakazawa. 2004. Perfluorooctane sulfonate (PFOS) and related perfluorinatedcompounds in human maternal and cord blood samples: assessment of PFOS exposure in a susceptible population during pregnancy. Environ. Health Perspect. 112:1204-1207. https://doi.org/10.1289/ehp.6864
  10. Kannan K, L Tao, E Sinclair, SD Pastva, DJ Jude and JP Giesy. 2005. Perfluorinatedcompounds in aquatic organisms at various trophic levels in a Great Lakes food chain. Arch. Environ. Contam. Toxicol. 48:559-566. https://doi.org/10.1007/s00244-004-0133-x
  11. Kim HN, WH Chung, CH Bae, KW Hwang and H Kim. 2006. Isolation and carbohydrate binding specificity of a lectin from the hemolymph of coastal crab Macrophthalmus japonicas. J. Pharm. Soc. Korea 50:166-171.
  12. Korea Ministry of Environment. 2007. 8th Endocrine disruptor research. Environmental health research department.
  13. Korea Ocean Research and Development Institute. 1992. On the cadmium bioaccumulation of the crab Macrophthalmus japonicas De Haan - low level calcium and salinity effects in muscle. (in Korean)
  14. Liu C, K Yu, X Shi, J Wang, PKS Lam, RSS Wu and B Zhon. 2007. Induction of oxidative stress and apoptosis by PFOS and PFOA in primary cultured hepatocytes of freshwater tilapia (Oreochromis niloticus). Aquat. Toxicol. 82:135-143. https://doi.org/10.1016/j.aquatox.2007.02.006
  15. Liu Y and Z Cui. 2010. Complete mitochondrial genome of the Asian paddle crab Charybdis japonica (Crustacea: Decapoda: Portunidae): Gene rearrangement of the marine brachyurans and phylogenetic considerations of the marine brachyurans and phylogenetic considerations of the decapods. Mol. Biol. Rep. 37:2559-2569. https://doi.org/10.1007/s11033-009-9773-2
  16. Mao H, FQ Tan, DH Wang, JQ Zhu, H Zhou and WX Yang. 2012. Expression and function analysis of metallothionein in the testis of stone crab Charybdis japonica exposed to cadmium. Aquat. Toxicol. 15:11-21.
  17. Martin JW and GE Davis. 2001. An updated classification of the recent crustacean. Natural History Museum of Los Angeles County, Los Angeles. pp. 124.
  18. Martin JW, DCG Muir, CA Moody, DA Ellis, WC Kwan, KR Solomon and SA Mabury. 2002. Collection of airbornefluorinated organics and analysis by gas chromatography/Chemical ionization mass spectrometry. Anal. Chem. 74:584-590. https://doi.org/10.1021/ac015630d
  19. Mhadhbi L, D Rial, S Perez and R Beiras. 2012. Ecological risk assessment of perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS) in marine environment using Isochrysisgalbana, Paracentrotus lividus, Siriella armata and Psetta maxima. J. Environ. Monit. 14:1375. https://doi.org/10.1039/c2em30037k
  20. Naile JE, JS Khim, S Hong, J Park, Bo Kwon, JS Ryu, JH Hwang, PD John and JP Giesy. 2013. Distributions and bio concentration characteristics of perfluorinated compounds-in environmental samples collected from the west coast of Korea. Chemosphere 90:387-394. https://doi.org/10.1016/j.chemosphere.2012.07.033
  21. Nakata H, K Kannan, T Nasu, HS Cho, E Sinclair and A Takemura. 2006. Perfluorinated contaminants in sediments and aquatic organisms collected from shallow water and tidal flat areas of the Ariake sea, Japan: environmental fate of perfluorooctane sulfonate in aquatic ecosystems. Environ. Sci. Technol. 40:4916-4921. https://doi.org/10.1021/es0603195
  22. Nakayama S, K Harada, K Inoue, K Sasaki, B Seery, N Saito and A Koizumi. 2005. Distribution of perfluorooctane acid (PFOA) and perfluorooctane sulfonate (PFOS) in Japan and their toxicities. Environ. Sci. 12:293-313.
  23. OECD. 2002. Hazard assessment of perfluorooctane sulfonate (PFOS) and its salts. ENV/JM/RD/7/FINAL. pp. 362.
  24. Ortiz-Zarragoitia M and MP Cajaraville. 2006. Biomarkers of exposure and reproduction-related effects in mussel exposed to endocrine disruptors. Arch. Environ. Contam. Toxicol. 50:361-369. https://doi.org/10.1007/s00244-005-1082-8
  25. Otani S, Y Kozuki, R Yamanaka, H Sasaoka, T Ishiyama, Y Okitsu, H Kakai and Y Fujiki. 2010. The role of crabs (Macrophthalmus japonicus) burrows on organiccarbon cycle in estuarine tidal flat. Estuar. Coast. Shelf Sci. 86:434-440. https://doi.org/10.1016/j.ecss.2009.07.033
  26. Park K and IS Kwak. 2013. Expression of stress response HSP70 gene in Asian paddle crabs, Charybdis japonica, exposure to endocrine disrupting chemicals, bisphenol A (BPA) and 4-nonlyphenol (NP). Ocean Sci. J. 48:207-214. https://doi.org/10.1007/s12601-013-0017-y
  27. Qu L, X Dong and F Guo. 2009. Automatic K-Means for Color Enteromorpha Image Segmentation. The 3rd International Conference on Intelligent Information Technology Application.
  28. Renzi M, C Guerranti, A Giovani, G Perra and SE Focardi. 2013. Perfluorinated compounds: levels, trophic web enrichments and human dietary intakes in transitional water ecosystems. Mar. Pollut. Bull. 76:146-157. https://doi.org/10.1016/j.marpolbul.2013.09.014
  29. Rho HS and W Kim. 2004. Marine Decapods of Gogunsan Island. Korean J. Environ. Biol. 22: 456-463. (in Korean)
  30. Seo IS and JS Hong. 2006. Species composition and seasonal variation of nektonic assemblages at the jangbong upper tidal flat, Incheon, Korea. Korea. J. Korea Soc. Oceanogr. 11:97-107. (in Korean)
  31. Stock NL, FK Lau, DA Ellis, JW Martin, DCG Muir and SA Madury. 2004. Polyfluorinated telomere alcohols and sulfonamides in the North American troposphere. Environ. Sci. Technol. 38:991-996. https://doi.org/10.1021/es034644t
  32. Strynar MJ and AB Lindstrom. 2008. Perfluorinated compounds in house dust from Ohio and North Carolina, USA. Environ. Sci. Technol. 42:3751-3756. https://doi.org/10.1021/es7032058
  33. Wang J, P Zhang, Q Shen, Q Wang, D Liu, J Li and L Wang. 2013. The effects of cadmium exposure on the oxidative state and cell death in the gill of freshwater crab Sinopotamon henanense. PloS One. 6:1-8.
  34. Yamashita N, K Kannan, S Taniyasu, Y Horii, G Petrick and T Gamo. 2005. A globalsurvey of perfluorinated acids in oceans. Mar. Pollut. Bull. 51:658-668. https://doi.org/10.1016/j.marpolbul.2005.04.026
  35. Zareitalabad P, J Siemens, M Hamer and W Amelung. 2013. Perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS) in surface waters, sediments, soils and wastewater - A review concentration and distribution coefficients. Chemosphere 91:725-73. https://doi.org/10.1016/j.chemosphere.2013.02.024
  36. Zhao YG, CKC Wong and MH Wong. 2012. Environment concentration, human exposure and body loadings of perfluorooctane sulfonate (PFOS), focusing on Asian countries. Chemosphere 89:355-368. https://doi.org/10.1016/j.chemosphere.2012.05.043