Cited by
- 객담을 이용한 Mycobacteria의 검출과 중합효소 연쇄반응의 민감성 비교 vol.47, pp.2, 2014, https://doi.org/10.15324/kjcls.2015.47.2.83
Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)
- Volume 46 Issue 2
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- Pages.47-53
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- 2014
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- 1738-3544(pISSN)
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- 2288-1662(eISSN)
DOI QR Code
Usefulness of PCR to Mycobacterium Tuberculous and Nontuberculous Mycobacteria from Paraffin-embedded Tissues
- Choi, Yeon-Il (Department of Clinical Pathology, Inha University Hospital) ;
- Kim, Hye-Young (Department of Clinical Laboratory Science, Shinsung University)
- Received : 2014.05.19
- Accepted : 2014.06.25
- Published : 2014.06.30
Abstract
The purpose of this study was to evaluate the clinical utility of TB/NTM PCR by comparing the results of TB PCR to detect Mycobacterium tuberculous (MTB) and nontuberculous mycobacteria (NTM) from paraffin-embedded tissue specimens. A total of 60 cases were tested using TB PCR and TB/NTM PCR. The MTB and NTM rate of TB/NTM PCR was 84.2% (16/19), 10.5% (2/19) in TB positive of TB PCR. The NTM rate of TB/NTM PCR was 29.3% (12/41) in TB negative of TB PCR. Fourteen different species of NTM were identified, the common isolate was M. gordonae (21.4%), M. avium (14.3%), M. ulcerans (7.1%), M. interjectum (7.1%), M. gilvum (7.1%), M. fortuitum (7.1%), M. mucogenicum (7.1%). The rare species identified were M. farcinogenes (7.1%), M. tokaiense (7.1%). Therefore, TB/NTM PCR is useful to differentiate MTB and NTM from paraffin-embedded tissue specimens and it is more effective in detecting NTM with TB PCR.