Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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The Antiproliferative Effects of Compounds Isolated from Schisandra chinensis

오미자로부터 분리된 화합물의 암세포 증식 억제 효과

  • Received : 2013.04.09
  • Accepted : 2013.12.03
  • Published : 2014.12.31
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Abstract

We isolated twelve lignans and three terpenoids were isolated from the n-hexane fraction of Schisandra chinensis extract. Using spectroscopic data and comparison with available literature, the following compounds were identified: (1) wuweizisu C, (2) gomisin N, (3) deoxyschisandrin, (4) gomisin A, (5) schisandrin, (6) chamigrenal, (7) schisanlactone D, (8) methylgomisin O, (9) angeloylgomisin O, (10) (-)-gomisin $L_2$, (11) schisandronic acid, (12) (-)-gomisin $L_1$, (13) (+)-gomisin $K_3$, (14) gomisin J, and (15) tigloylgomisin H. Notably, this was the first finding that compound (8) was isolated from this plant. Each compound was evaluated for its in vitro cytotoxic activities toward HL-60 (human leukemia), HeLa (human cervical carcinoma), and MCF-7 (breast cancer) cell lines. Compounds (7), (8), and (9) exhibited strong cytotoxic effects on HL-60 ($IC_{50}$ 7.37, 6.60, and $8.00{\mu}M$, respectively), whereas compound (6) exhibited weak cytotoxicity towards MCF-7 ($IC_{50}$ $30.50{\mu}M$). In addition, compound (8) showed the strongest activity towards HeLa cells ($IC_{50}$ $1.46{\mu}M$).

오미자 추출물의 n-hexane 분획으로부터 open column chromatography, 분취용 HPLC 등 여러 가지 크로마토그라피를 이용하여 총 15종의 화합물을 분리하였다. 분리된 화합물들은 각각의 기기분석 데이터를 문헌치와 비교하여, 각각 wuweizisu C (1), gomisin N (2), deoxyschisandrin (3), gomisin A (4), schisandrin (5), chamigrenal (6), schisanlactone D (7), methylgomisin O (8), angeloylgomisin O (9), (-)-gomisin $L_2$ (10), schisandronic acid (11), (-)-gomisin $L_1$ (12), (+)-gomisin $K_3$ (13), gomisin J (14), tigloylgomisin H (15)으로 동정하였다. 이들 중 methylgomisin O (8)는 이 식물에서 처음으로 분리되었다. 분리된 화합물들은 HL-60 (human leukemia), HeLa (human cervical carcinoma) 및 MCF-7 (breast cancer cells) 세포주에 대하여 세포독성 실험을 실시하였다. 그 결과 화합물 7, 8 및 9는 HL-60 세포주에 대하여 각각 7.37, 6.60 및 $8.00{\mu}M$의 $IC_{50}$로 비교적 강한 세포독성 효과를 나타냈다. 화합물 6은 MCF-7에 대하여 $IC_{50}$ $30.50{\mu}M$로 적정한 세포독성을 나타냈다. 그리고 화합물 8은 HeLa cells에 대하여 $IC_{50}$ $1.46{\mu}M$로 비교적 강한 세포 독성으로 나타냈다.

Keywords

References

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