한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제42권4호
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  • Pages.407-412
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  • 2014
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  • 1598-642X(pISSN)
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  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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누룩으로부터 분리된 전분대사 효모 Saccharomycopsis fibuligera 균주의 생육특성

Characterization of Starch-Utilizing Yeast Saccharomycopsis fibuligera Isolated from Nuruk

  • 최다혜 (강원대학교 식품생명공학과) ;
  • 박은희 (강원대학교 식품생명공학과) ;
  • 김명동 (강원대학교 식품생명공학과)
  • Choi, Da-Hye (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Park, Eun-Hee (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Kim, Myoung-Dong (Department of Food Science and Biotechnology, Kangwon National University)
  • 투고 : 2014.09.25
  • 심사 : 2014.09.27
  • 발행 : 2014.12.28
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초록

누룩으로부터 MBY1276, 1280, 1282, 1320, 1322, 1324 및 MBY 1327로 각각 명명된 전분을 분해하는 효모균주를 분리하였다. 이들 균주는 18S rRNA 영역의 ITS 단편 및 26S rDNA 영역의 D1/D2 단편의 염기서열 해석 및 탄소원 대사특성 분석을 통하여 S. fibuligera로 동정하였다. 상주지역에서 수집된 누룩으로부터 분리된 MBY1320 균주는 대조구 균주인 S. fibuligera KCTC7806 및 누룩으로부터 분리된 다른 S. fibuligera 균주에 비해 고온에서 상대적으로 높은 비성장속도를 나타내었다. 전분을 분해하는데 필요한 효소인 ${\alpha}$-amylase 및 glucoamylase 효소활성을 측정한 결과 MBY1320 균주의 ${\alpha}$-amylase 효소활성은 대조구 균주보다 낮지만 glucoamylase 효소활성은 고온에서 상대적으로 우수한 것으로 나타났다. 효소활성 분석결과는 MBY1320 균주가 표준균주 및 다른 S. fibuligera 균주와 비교하여 고온에서 상대적으로 높은 비성장속도를 나타내는 것을 뒷받침하는 결과로 판단되었다. 가용성 전분을 이용한 회분식 배양 결과 MBY1320 균주는 표준균주 보다 $42^{\circ}C$에서 우수한 성장속도 및 에탄올 생산속도를 나타내었다. 본 연구를 통하여 기존에 보고된 S. fibuligera 균주보다 고온에서 glucoamylase 효소활성 및 비성장속도가 우수한 S. fibuligera 균주를 보고하는 바이다.

A number of Saccharomycopsis fibuligera strains that can hydrolyse and utilize starch as a carbon source were isolated from nuruk, a traditional Korean starter for rice wine fermentation, and their specific growth rates on starch-containing medium were compared to choose the prominent strain. S. fibuligera strain MBY1320 showed a higher growth rate at $42^{\circ}C$ than that of strain S. fibuligera KCTC7806, indicating that S. fibuligera MBY1320 has more thermo-tolerant machinery for starch hydrolysis and utilization than KCTC7806. Although the activity of ${\alpha}$-amylase at $30^{\circ}C$ was significantly lower for S. fibuligera MBY1320 than KCTC7806 (3,812.5 U vs. 14,878.5 U), S. fibuligera MBY1320 showed a much higher glucoamylase activity at $42^{\circ}C$ than S. fibuligera KCTC7806 (5,048.9 U vs. 13,152.3 U). Thus, a new S. fibuligera strain, with a higher starch-hydrolysing activity at elevated temperatures than that of other types of strain, this study reports.

키워드

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  3. 실험실 적응진화를 이용한 Saccharomycopsis fibuligera의 젖산에 대한 내성 증대 vol.44, pp.4, 2016, https://doi.org/10.4014/mbl.1608.08001
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  10. 누룩으로부터 자일리톨 생산능이 있는 내열성 효모 Millerozyma farinosa 균주의 분리 vol.47, pp.4, 2014, https://doi.org/10.4014/mbl.1902.02006
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