DOI QR코드

DOI QR Code

A Study on the Oral Toxicity of Mecasin in Rats

  • Jeong, Hohyun (Department of Acupuncture & Moxibustion Medicine, Wonkwang University Gwangju Korean Medical Hospital) ;
  • Lee, Jongchul (Department of Acupuncture & Moxibustion Medicine, Wonkwang University Gwangju Korean Medical Hospital) ;
  • Cha, Eunhye (Department of Acupuncture & Moxibustion Medicine, Wonkwang University Gwangju Korean Medical Hospital) ;
  • Park, Manyong (Department of Acupuncture & Moxibustion Medicine, Wonkwang University Gwangju Korean Medical Hospital) ;
  • Son, Ilhong (Department of Neurology, Inam Neuroscience Research Center, Sanbon Medical Center, College of Medicine, Wonkwang University) ;
  • Song, Bongkeun (Department of Internal Medicine, Wonkwang University Gwangju Korean Medical Hospital) ;
  • Kim, Sungchul (Department of Acupuncture & Moxibustion Medicine, Wonkwang University Gwangju Korean Medical Hospital)
  • Received : 2014.08.20
  • Accepted : 2014.09.17
  • Published : 2014.12.30

Abstract

Objectives: In this study, we investigated the oral toxicity of Gami-Jakyak Gamcho buja Decoction (Mecasin) to develop safe treatments. Methods: All experiments were conducted at the Medvill, an institution authorized to perform non-clinical studies, under the Good Laboratory Practice (GLP) regulations. In order to investigate the oral toxicity of Mecasin, we administered Mecasin orally to rats. Sprague-Dawley rats were divided into four groups of five male and five female animals per group: group 1 being the control group and groups 2, 3, and 4 being the experimental groups. Doses of Mecasin, 500 mg/kg, 1,000 mg/kg and 2,000 mg/kg, were administered to the experimental groups, and a dose of normal saline solution, 10 mL/kg, was administered to the control group. We examined the survival rate, weight, clinical signs, and gross findings. This study was conducted under the approval of the Institutional Animal Ethics Committee. Results: No deaths or abnormalities occurred in any of the four groups. Although slight decreases in the weights of some female rats were noted on the third day, no significant changes in weights or gross findings between the control group and the experimental groups were observed. To check for abnormalities in organs, we used microscopy to examine representative histological sections of each specified organ; the results showed no significant differences in any of the organs. Conclusion: The results showed that administration of 500 - 2,000 mg/kg of Mecasin did not cause any changes in weight or in the results of necropsy examinations. It also did not result in any mortalities. The above findings suggest that treatment with Mecasin is relatively safe. Further studies on this subject are needed to yield more concrete evidence.

Keywords

References

  1. Guo L, Cho SY, Kang SS, Lee SH, Baek HY, Kim YS. Orthogonal array design for optimizing extraction efficiency of active constituents from jakyak-gamcho decoction, the complex formula of herbal medicines, paeoniae radix and glycyrrhizae radix. J Ethnopharmacol. 2007;113(2):306-11. https://doi.org/10.1016/j.jep.2007.06.013
  2. Kang TH, Baek HY, Kim YC. Protective effect of jakyak-gamcho-tang extract and its constituents against t-BHP-induced oxidative damage in HT22 cells. Am J Chin Med. 2005;33(2):181-9. https://doi.org/10.1142/S0192415X05002850
  3. Lee JM, Hong SY, Oh MS. Effects of jakyakkamchobuja-tang on papain-induced osteoarthritis in mice. J Korean Oriental Med. 2013;34(1):116-35.
  4. Elinav E, Chajek-Shaul T. Licorice consumption causing severe hypokalemic paralysis. Mayo Clin Proc. 2003;78(6):767-8. https://doi.org/10.4065/78.6.767
  5. Kim JK, Kim SH, Lee SM, Jeong HY, Park MY, Kim DW, et al. Study of single-dose toxicity of aconitum kusnezoffii reichb. pharmacopuncture in rats. J Pharmacopunct. 2012;15(3):48-52. https://doi.org/10.3831/KPI.2012.15.007
  6. Sun JK, Shin MK. Cytotoxic effect of salvia miltiorrhiza root against L1210 cell. J Orient Neuropsychiatry. 1992;3(1):84-90.
  7. Roh HS, Jeong JY, Seok Jh, Ha HY. Acute oral toxicity of root of polygala teunifolia wild. extract. J Orient Obstet Gynecol. 2013;26(4):1-13.
  8. Kim YH, Seo BI. A philological study on poisoning and side effects of gastrodiae rhizoma. The Journal of Jeahan Oriental Medical Academy. 2012;10(1):137-45.
  9. Choi EY, Oh HJ, Park NK, Chun HJ, Ahn JW, Jeon BH, et al. [Screening of cytotoxicity and antimicrobial effects of extracts from the atractylodes macrocephala koidz]. J Orient Physiol Pathol. 2002;16(2):348-52. Korean.
  10. Fang YS, Shan DM, Liu JW, Xu W, Li CL, Wu HZ, et al. Effect of constituents from fructus aurantii immaturus and radix paeoniae alba on gastrointestinal movement. Planta Med. 2009;75(1):24-31. https://doi.org/10.1055/s-0028-1088342
  11. Huang KS, Lin JG, Lee HC, Tsai FJ, Bau DT, Huang CY, et al. Paeoniae alba radix promotes peripheral nerve regeneration. Evid Based Complement Alternat Med. 2011;109809:1-8.
  12. Park HJ, Shim HS, Kim H, Kim KS, Lee H, Hahm DH, et al. Effects of glycyrrhizae radix on repeated restraint stress-induced neurochemical and behavioral responses. Korean J Physiol Pharmacol. 2010;14(6):371-6. https://doi.org/10.4196/kjpp.2010.14.6.371
  13. Tong P, Wu C, Wang X, Hu H, Jin H, Li C, et al. Development and assessment of a complete-detoxi arthritis cation strategy for fuzi (lateral root of aconitum carmichaeli) and its application in rheumatoid therapy. J Ethnopharmacol. 2013;146(2):562-71. https://doi.org/10.1016/j.jep.2013.01.025
  14. J. Singhuber, M. Zhu, S. Prinz, B. Kopp. Aconitum in traditional chinese medicine: a valuable drug or an unpredictable risk?. J Ethnopharmacol. 2009;126(1):18-30. https://doi.org/10.1016/j.jep.2009.07.031
  15. Kim DR, Kim HY, Park JK, Park SK, Chang MS. Aconiti lateralis preparata radix activates the proliferation of mouse bone marrow mesenchymal stem cells and induces osteogenic lineage differentiation through the bone morphogenetic protein-2/smad-dependent run$\times$2 pathway. Evid Based Complement Alternat Med. 2013;586741:1-11.
  16. Hu F, Koon CM, Chan JY, Lau KM, Fung KP. The cardioprotective effect of danshen and gegen decoction on rat hearts and cardiomyocytes with post-ischemia reperfusion injury. BMC Complement Altern Med. 2012;12:249. https://doi.org/10.1186/1472-6882-12-249
  17. Lee HG, Kim YS. Effects of salvia miltriorrhiza radix on neuronal apoptosis following intracerebral hemorrhage of rats. Kor J Herbology. 2012;27(3):89-94. https://doi.org/10.6116/kjh.2012.27.3.89
  18. Miao Q. New advance in identification of chinese medicine tianma. China Medical Herald. 2011;8(20):20-5.
  19. Tang XH, Li B, Hu LY, Hu Zuo, YE Q, ZHU H, et al. [The compatibility applications of tianma]. Journal of Shanxi College of Traditional Chinese Medicine. 2012;13(3):75-6. Chinese.
  20. Shin IJ, Son SU, Park H, Kim Y, Park SH, Swanberg K, et al. Preclinical evidence of rapid-onset antidepressant-like effect in radix polygalae extract. Plos One. 2014;9(2):e88617. https://doi.org/10.1371/journal.pone.0088617
  21. Qin L, Yang YB, Tuo QH, Zhu BY, Chen LX, Zhang L, et al. Effects and underlying mechanisms of curcumin on the proliferation of vascular smooth muscle cells induced by chol:MbetaCD. Biochem Biophys Res Commun. 2009;379(2):277-82. https://doi.org/10.1016/j.bbrc.2008.12.038
  22. Ryu HW, Kim YS, Lim EM. The Anti inflammatory Effects of Chaenomelis Fructus Herba Water Extract on Mouse RAW 264.7 Cell. J Orient Obstet Gynecol. 2012; 25(3): 1-15.
  23. Shin DS, Kim YI, Lee H. [Effect of herbal-acupuncture with chaenomelis fructus solution at the joksamni of mice with collagen-induced arthritis]. The Acupuncture. 2007;24(3):127-43. Korean.
  24. Kim YK. [The principle and test method. toxicology]. Seoul: Donghwagisul; 1994(3). p. 15-8. Korean.
  25. Kim YG. [Toxicology]. Seoul: Donghwagisul; 1984. p. 15-8. Korean.
  26. Korea Food & Drug Administration notification. Seoul: Korea Food & Drug Administration; 2005. p. 60.

Cited by

  1. A 4-week Repeated dose Oral Toxicity Study of Mecasin in Sprague-Dawley Rats to Determine the Appropriate Doses for a 13-week, Repeated Toxicity Test vol.18, pp.4, 2015, https://doi.org/10.3831/KPI.2015.18.037
  2. A Study on Single Dose Toxicity of Intravenous Injection of Mecasin Herbal Acupuncture vol.33, pp.1, 2016, https://doi.org/10.13045/acupunct.2016001
  3. A Study on Single Dose Toxicity of Mecasin Pharmacopuncture Injection in Muscle vol.36, pp.2, 2015, https://doi.org/10.13048/jkm.15012
  4. Mecasin treatment in patients with amyotrophic lateral sclerosis: study protocol for a randomized controlled trial vol.19, pp.1, 2018, https://doi.org/10.1186/s13063-018-2557-z
  5. KCHO-1, a novel herbal anti-inflammatory compound, attenuates oxidative stress in an animal model of amyotrophic lateral sclerosis vol.18, pp.4, 2017, https://doi.org/10.4142/jvs.2017.18.4.487