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Residual Characteristics of a Systemic Insecticide Flonicamid and Its Metabolites in Sweet Pepper

착색단고추 중 침투성농약 플로니카미드 및 대사물질의 생성 및 잔류양상

  • Seo, Eun-Kyung (Department of Agro-food Safety, National Academy of Agriculture Science, Rural Development Administration) ;
  • Kim, Taek-Kyum (Department of Agro-food Safety, National Academy of Agriculture Science, Rural Development Administration) ;
  • Hong, Su-Myeong (Department of Agro-food Safety, National Academy of Agriculture Science, Rural Development Administration) ;
  • Kwon, Hye-Young (Department of Agro-food Safety, National Academy of Agriculture Science, Rural Development Administration) ;
  • Gwon, Ji-Hyeong (Department of Agro-food Safety, National Academy of Agriculture Science, Rural Development Administration) ;
  • Cho, Nam-Jun (Department of Agro-food Safety, National Academy of Agriculture Science, Rural Development Administration)
  • 서은경 (농촌진흥청 국립농업과학원 농산물안전성부) ;
  • 김택겸 (농촌진흥청 국립농업과학원 농산물안전성부) ;
  • 홍수명 (농촌진흥청 국립농업과학원 농산물안전성부) ;
  • 권혜영 (농촌진흥청 국립농업과학원 농산물안전성부) ;
  • 권지형 (농촌진흥청 국립농업과학원 농산물안전성부) ;
  • 조남준 (농촌진흥청 국립농업과학원 농산물안전성부)
  • Received : 2014.11.04
  • Accepted : 2014.11.21
  • Published : 2014.12.31

Abstract

The residue levels of flonicamid and its metabolites, 4-(trifluoromethyl)nicotinic acid (TFNA) and N-4-(trifluoromethyl)nicotinoyl glycine (TFNG) in sweer pepper were investigated to examine the residual characteristics of analytes for 87 days after pesticide application. The pesticide was applied once at recommended dosage and double dosage by foliar sprays and the samples of fruits and leaves of sweet pepper were collected for each treatment. The residues of flonicamid in all of fruits and leaves decreased gradually over time, while the residue levels of TFNG metabolite exhibited tendency that increased for long periods and thereafter decreased. Total flonicamid residual concentrations containing metabolites residues in fruit samples increased consistently until 30 days post-application and higher residue levels than residues at 1 day post-application were detected from 30 day to 87 day after treatment. The residue pattern observed in fruit could be explained by the movement of TFNG from leaves to fruits of plant. Such residual characteristic was similarly found in samples treated both recommended dosage and double dosage.

착색단고추 중 침투성농약 flonicamid의 잔류특성을 구명하기 위하여 flonicamid 및 그 대사물질의 잔류농도를 분석하고 경시적인 잔류량 변화양상을 조사하였다. 플로니카미드 10% 입상수화제를 추천사용농도 및 그의 2배 농도로 경엽처리하고, 살포 후 1, 7, 15, 30, 50, 70, 87일의 총 7회에 걸쳐 과실, 상엽, 하엽을 구분하여 시료를 채취하여 잔류농도를 분석하였고 살포 후 87일간의 잔류량 변화를 조사하였다. 과실, 상엽, 하엽 모두에서 모화합물인 flonicamid의 잔류량은 1일차에 최고치를 나타낸 이후 급격히 감소되는 경향을 보인 반면, 대사물질 TFNG의 잔류량은 지속적으로 증가하여 15-30일차에 최고 잔류량을 나타내었고 살포 후 87일 이후에도 초기 잔류량 보다 높은 농도를 유지하였다. 과실 중 총 flonicamid의 잔류량이 시간 경과에 따라 점차적으로 증가하여 살포 후 87일까지 높은 농도를 유지하는 잔류양상은 착색단고추 잎 중에 높은 농도로 잔류하는 대사물질 TFNG의 이동에 의한 결과로 보여지며, 이러한 잔류량 변화는 기준량 및 2배량 처리구에서 유사한 양상을 나타내었다.

Keywords

References

  1. Anastassiades, M., S. J. Lehotay, D. Stajnbaher, and F. J. Schenck (2003) Fast and Easy multiresidue method employing acetonitrile extraction/partitioning and "dispersive solidphase extraction" for the determination of pesticide residues in produce. J AOAC Int. 86:412-431.
  2. Bae, C. H., K. W. Cho, Y. S. Kim, H. J. Park, K. S. Shin, Y. K. Park and K. S. Lee (2013) Honeybee toxicity by residues on tomato folige of systemic insecticides applied to the soil. Korean J Pestic Sci. 17(3):178-183. https://doi.org/10.7585/kjps.2013.17.3.178
  3. Choi, M. Y., J. H. Kim, H. Y. Kim, Y. W. Byeon and Y. H. Lee (2009) Biological control based IPM of insect pests on sweet pepper in greenhouse in summer. Korean J Appl Entomol 48(4):503-508. https://doi.org/10.5656/KSAE.2009.48.4.503
  4. European Food Safety Authority (2010) Reasoned opinion modification of the existing MRLs for fonicamid in various crops. Summary of EFSA Journal 8:1610. https://doi.org/10.2903/j.efsa.2010.1610
  5. Jansen, J. P., T. Defrance and A. M. Warnier (2011) Side effects of flonicamid and pymetrozine on five aphid natural enemy species. BioControl 56:757-770.
  6. Japan food safety commission (2008) The 40th pesticides expert committee executive committee - flonicamid test valuation report. (second edition)
  7. Kamel, A. (2010) Refined methodology for the determination of neonicotinoid pesticides and their metabolites in Honey Bees and Bee products by liquid chromatography-tandem mass spectrometry (LC-MS/MS). J Agric Food Chem. 58(10):5926-5931. https://doi.org/10.1021/jf904120n
  8. Ko, A. Y., A. M. Abd El-Aty, M. d. Musfiqur Rahman, J. Jang, S. W. Kim, J. H. Choi, and J. H. Shim (2014) A modified QuEChERS method for simultaneous determination of flonicamid and its metabolites in paprika using tandem mass spectrometry. Food Chem. 157:413-420. https://doi.org/10.1016/j.foodchem.2014.02.038
  9. Korea crop protection association (2012) Pesticide use manual. p 741.
  10. Lee, S. W., J. H. Choi, S. K. Cho, H. A. Yu, A. M. Abd El-Aty and J. H. Shim (2011) Development of a new QuEChERS method based on dry ice for the determination of 168 pesticides in paprika using tandem mass spectrometry. J Chromatogr A. 1218:4366-4377. https://doi.org/10.1016/j.chroma.2011.05.021
  11. Lehotay, S. J. (2007) Determination of pesticide residues in foods by acetonitrile extraction and partitioning with magnesium sulfate: collaborative study. J AOAC Int. 90(2):485-520.
  12. Lehotay, S. J., K. A. Son, H. Y. Kwon, U. Koesukwiwat, W. Fu, K. Mastovska, E. Hoh and N. Leepipatpiboon (2010) Comparison of QuEChERS sample preparation methods for the analysis of pesticide residues in fruits and vegetables. J Chromatogr A. 1217:2548-2560. https://doi.org/10.1016/j.chroma.2010.01.044
  13. Morita, M., T. Ueda, T. Yoneda, T. Koyanagi and T. Haga (2007) Flonicamid, a novel insecticide with a rapid inhibitory effect on aphid feeding. Pest Manag Sci 63:969-973. https://doi.org/10.1002/ps.1423
  14. Rural Development Administration (2012) Safe Pesticide Application Manual for Exporting Agricultural Products. p 22.
  15. SANCO (2009) Method validation and quality control procedures for pesticide residues analysis in food and feed.
  16. Son, K. A., H. Y. Kwon, Y. D. Jin, B. J. Park, J. B. Kim, J. H. Park, T. K. Kim, G. J. Im and K. W. Lee (2013) The Behaviour of Residues of Flonicamid and Metabolites in Sweet peppers. Korean J Pestic Sci. 17(3):145-154. https://doi.org/10.7585/kjps.2013.17.3.145