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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Effects and Optimization of Gamma-Amino Butyric Acid (GABA) Production Process using Glutamate Decarboxylase (GAD)

Glutamate Decarboxylase (GAD)를 이용한 Gamma-Amino Butyric Acid (GABA) 생산 및 최적화

  • Kim, Eui Jin (Department of Chemical & Biochemical Engineering, Chosun University) ;
  • Lee, Jung-Heon (Department of Chemical & Biochemical Engineering, Chosun University)
  • 김의진 (조선대학교 생명화학공학과) ;
  • 이중헌 (조선대학교 생명화학공학과)
  • Received : 2014.10.14
  • Accepted : 2014.11.10
  • Published : 2014.12.30
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Abstract

pH controlled batch reactor and bubble column reactors have been developed in this research. They were used to produce high concentration of GABA and to determine optimal pH for GABA production. Glutamate decarboxylase (GAD) was isolated from recombinant E. coli and used for GABA production from monosodium glutamate (MSG). pH control was inevitable because the pH increased with MSG consumption. GAD showed highest activity at acidic conditions at pH 5.5 but the optimal pH for GABA production was pH 6.0. When 1.5 mole of MSG was used as reactant, the 1.05 mole of GABA was produced after 10 hrs batch reaction. Using bubble column reactors, 80 % of MSG was converted to GABA for 6 hrs reaction and 1.2 mole of GABA was produced.

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References

  1. Aoki, H., Y. Furuya, Y. Endo, and K. Fujimoto (2003) Effect of - Aminobutyric Acid-enriched Tempeh-like Fermented Soybean (GABA-Tempeh) on the Blood Pressure of Spontaneously Hypertensive Rats. Biosci. Biotechnol. Bioche. 67: 1806-1808. https://doi.org/10.1271/bbb.67.1806
  2. Chang, J. S., B. S. Lee, and Y. G. Kim (1992) Changes in gamma-aminobutyric Acid (GABA) and the Main Constituents by a Treatment Conditions and of Anaerobically Treated Green Tea Leaves. Korean J. Food Sci. Technol. 24: 315-319.
  3. Nakagawa, T., T. Yokozawa, H.J. Kim, and N. Shibahara (2005) Protective Effects of $\gamma$-Aminobutyric Acid in Rats with Streptozotocin-Induced Diabetes. J. Nutr. Sci. Vitaminol. 51: 278-282. https://doi.org/10.3177/jnsv.51.278
  4. Watanabe, H., A. Suzuki, M. Goto, D. Lubahn, H. Handa, and T. Iguchi (2004) Tissue-specific estrogenic and non-estrogenic effects of a xenoestrogen, nonylphenol. J. Mol. Endocrinol. 33: 243-252. https://doi.org/10.1677/jme.0.0330243
  5. Rissman, R. A., A. L. De Blas, and D. M. Armstrong (2007) GABA receptors in aging and Alzheimer's disease. J. Neurochem. 103: 1285-1292. https://doi.org/10.1111/j.1471-4159.2007.04832.x
  6. Obata, K., M. Hirono, N. Kume, Y. Kawaguchi, S. Itohara, and Y. Yanagawa (2008) GABA and synaptic inhibition of mouse cerebellum lacking glutamate decarboxylase 67. Biochem. Biophy. Res. Commun. 370: 429-433. https://doi.org/10.1016/j.bbrc.2008.03.110
  7. Hasler, G., J. van der Veen, T. Tumonis, N. Meyers, J. Shen, and W. C. Drevets (2007) REduced prefrontal glutamate/glutamine and -aminobutyric acid levels in major depression determined using proton magnetic resonance spectroscopy. Archiv. General Psychiatry 64: 193-200. https://doi.org/10.1001/archpsyc.64.2.193
  8. Jeng, K. C., C. S. Chen, Y. P. Fang, R. C. W. Hou, and Y. S. Chen (2007) Effect of microbial fermentation on content of statin, GABA, and polyphenols in Pu-Erh tea. J. Agric. Food Chem. 55: 8787-8792. https://doi.org/10.1021/jf071629p
  9. Komatsuzaki, N., K. Tsukahara, H. Toyoshima, T. Suzuki, N. Shimizu, and T. Kimura (2007) Effect of soaking and gaseous treatment on GABA content in germinated brown rice. J. Food Eng. 78: 556-560. https://doi.org/10.1016/j.jfoodeng.2005.10.036
  10. Chung, H. J., S. H. Jang, H. Y. Cho, and S. T. Lim (2009) Effects of steeping and anaerobic treatment on GABA (-aminobutyric acid) content in germinated waxy hull-less barley. LWT- Food Sci. Technol. 42: 1712-1716. https://doi.org/10.1016/j.lwt.2009.04.007
  11. Youn, Y. S., J. K. Park, H. D. Jang, and Y. W. Rhee (2011) Sequential hydration with anaerobic and heat treatment increases GABA (-aminobutyric acid) content in wheat. Food Chem. 129: 1631-1635. https://doi.org/10.1016/j.foodchem.2011.06.020
  12. Chuang, C. Y., Y. C. Shi, H. P. You, Y. H. Lo, and T. M. Pan (2011) Antidepressant effect of GABA-rich monascus-fermented product on forced swimming rat model. J. Agric. Food Chem. 59: 3027-3034. https://doi.org/10.1021/jf104239m
  13. Eckstein, J. A., G. M. Ammerman, J. M. Reveles, and B. L. Ackermann (2008) Analysis of glutamine, glutamate, pyroglutamate, and GABA in cerebrospinal fluid using ion pairing HPLC with positive electrospray LC/MS/MS. J. Neurosci Methods 171: 190-196. https://doi.org/10.1016/j.jneumeth.2008.02.019
  14. Hao, R. and J. C. Schmit (1993) Cloning of the gene for glutamate decarboxylase and its expression during conidiation in Neurospora crassa. Biochem. J. 293: 735-738. https://doi.org/10.1042/bj2930735
  15. Park, S., E. Kim, W. Noh, Y. Oh, H. Kim, B. Song, K. Cho, S. Hong, S. Lee, and J. Jegal, (2013) Synthesis of nylon 4 from gamma-aminobutyrate (GABA) produced by recombinant Escherichia coli. Bioproc. Biosyst. Eng. 36: 885-892. https://doi.org/10.1007/s00449-012-0821-2
  16. Le Vo, T., T. Kim, and S. Hong (2012) Effects of glutamate decarboxylase and gamma-aminobutyric acid (GABA) transporter on the bioconversion of GABA in engineered Escherichia coli. Bioproc. Biosyst. Eng. 35: 645-650. https://doi.org/10.1007/s00449-011-0634-8
  17. Fenalti, G., R. H. P. Law, A. M. Buckle, C. Langendorf, K. Tuck, C. J. Rosado, N. G. Faux, K. Mahmood, C. S. Hampe, J. P. Banga, M. Wilce, J. Schmidberger, J. Rossjohn, O. El-Kabbani, R. N. Pike, A. I. Smith, I. R. Mackay, M. J. Rowley, and J. C. Whisstock, (2007) GABA production by glutamic acid decarboxylase is regulated by a dynamic catalytic loop. Nat. Struct. Mol. Biol. 14: 280-286. https://doi.org/10.1038/nsmb1228
  18. Yuan, P. Q., C. Grns, L. Kllstrm, J. Yu, K. Huhman, D. Larhammar, H. E. Albers, and A. E. Johnson (1997) Differential distribution of glutamate decarboxylase-65 and glutamate decarboxylase-67 messenger RNAs in the entopeduncular nucleus of the rat. Neurosci. 78: 87-97. https://doi.org/10.1016/S0306-4522(96)00593-3
  19. Dkhissi, O., J. F. Julien, M. Wasowicz, N. Dalil-Thiney, J. Nguyen-Legros, and C. Versaux-Botteri (2001) Differential expression of GAD65 and GAD67 during the development of the rat retina. Brain Res. 919: 242-249. https://doi.org/10.1016/S0006-8993(01)03022-0
  20. Yao, W., X. Wu, J. Zhu, B. Sun, and C. Miller (2013) In vitro enzymatic conversion of -aminobutyric acid immobilization of glutamate decarboxylase with bacterial cellulose membrane (BCM) and non-linear model establishment. Enzyme Microb. Technol. 52: 258-264. https://doi.org/10.1016/j.enzmictec.2013.01.008
  21. Ling, D., G. Wu, C. Wang, F. Wang, and G. Song (2000) The preparation and characterization of an immobilized l-glutamic decarboxylase and its application for determination of l-glutamic acid. Enzyme Microb. Technol. 27: 516-521. https://doi.org/10.1016/S0141-0229(00)00242-8
  22. Lambrecht, R. H. D., G. Slegers, G. Mannens, and A. Claeys (1987) Immobilization of glutamate decarboxylase and the preparation of an enzyme column for the synthesis of -[13N]aminobutyric acid. Enzyme Microb. Technol. 9: 221-224. https://doi.org/10.1016/0141-0229(87)90019-6
  23. Jun, C., J. C. Joo, J. H. Lee, and Y. H. Kim (2014) Thermostabilization of glutamate decarboxylase B from Escherichia coli by structure-guided design of its pH-responsive N-terminal interdomain. J. Biotechnol. 174: 22-28. https://doi.org/10.1016/j.jbiotec.2014.01.020
  24. Gioia, M. G., P. Andreatta, S. Boschetti, and R. Gatti (2007) Development and validation of a liquid chromatographic method for the determination of branched-chain amino acids in new dosage forms. Journal of Pharmaceutical and Biomedical Analysis 45: 456-464. https://doi.org/10.1016/j.jpba.2007.07.016
  25. Tavaria, F. K., I. Franco, F. Javier Carballo, and F. Xavier Malcata (2003) Amino acid and soluble nitrogen evolution throughout ripening of Serra da Estrela cheese. Int. Dairy J. 13: 537-545. https://doi.org/10.1016/S0958-6946(03)00060-8
  26. Komatsuzaki, N., J. Shima, S. Kawamoto, H. Momose, and T. Kimura (2005) Production of -aminobutyric acid (GABA) by Lactobacillus paracasei isolated from traditional fermented foods. Food Microbiol. 22: 497-504. https://doi.org/10.1016/j.fm.2005.01.002
  27. Kang, T. J., N. A. T. Ho, and S. P. Pack (2013) Buffer-free production of gamma-aminobutyric acid using an engineered glutamate decarboxylase from Escherichia coli. Enzyme Microb. Technol. 53: 200-205. https://doi.org/10.1016/j.enzmictec.2013.04.006

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