Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Development and of Diagnostic System for Detection of Cowpea chlorotic mottle virus using by Nested PCR

Nested PCR을 이용한 Cowpea chlorotic mottle virus 정밀 진단 시스템 개발

  • Min, Byung-Dae (Environmental Infrastructure Research Department, National Institute of Environmental Research) ;
  • Kim, Young-Suk (Yeongsan River Environment Research Center, National Institute of Environmental Research) ;
  • Lee, Siwon (Environmental Infrastructure Research Department, National Institute of Environmental Research) ;
  • Lee, Su-Heon (School of Applied Biosciences, Kyungpook National University)
  • 민병대 (국립환경과학원 환경기반연구부) ;
  • 김영석 (국립환경과학원 영산강물환경연구소) ;
  • 이시원 (국립환경과학원 환경기반연구부) ;
  • 이수헌 (경북대학교 응용생명과학부)
  • Received : 2014.09.04
  • Accepted : 2014.12.31
  • Published : 2014.12.31
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Abstract

Cowper chlorotic mottle virus (CCMV) is the 'controlled' quarantine virus as plant pathogenic virus that are classed as group VI (+) ssRNA virus that belongs to the genus Bromovirus and family Bromoviridae, When plants that are Phaseolus vulgaris, Clitoria ternatea, Nicotiana tabaccum, Glycine max, Vigna unguiculata and Vigna siensis, and Arachis hypogaea is imported in domestic. In this study, inspection system is implemented to analyze CCMV accurately and rapidly by developing RT-PCR, nested PCR, and gene insertion positive control. It is expected that the method developed in this study will contribute to the plant quarantine to be consistently utilized in the field.

Cowpea chlorotic mottle virus (CCMV)는 Group IV positive sense single strand RNA virus, Bromoviridae과, Bromovirus속으로 분류하는 식물병원성 바이러스로, 강낭콩(Phaseolus vulgaris), 나비완두(Clitoria ternatea), 담배(Nicotiana tabaccum), 대두(Glycine max), 동부(Vigna unguiculata, Vigna siensis) 및 땅콩(Arachis hypogaea)이 국내로 수입될 경우, 검사를 수행하는 관리급 검역바이러스이다. 본 연구에서는, RT-PCR, nested PCR 및 유전자-삽입 양성대조구를 개발하여, CCMV를 현장에서 신속, 정확하게 진단할 수 있는 정밀검정 시스템을 구현하였다. 본 연구에서 개발한 방법은 지속적으로 현장에서 활용되어 식물검역에 기여할 것이라고 기대된다.

Keywords

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