Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Effect of temperature and relative humidity in refrigerator on quality traits and storage characteristics of Pre-packed Hanwoo loin

냉장고 내 온도와 상대습도가 포장 한우 등심의 품질 및 저장 특성에 미치는 영향

  • Seol, Kuk-Hwan (National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Ki Hyun (National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Young Hwa (National Institute of Animal Science, Rural Development Administration) ;
  • Youm, Kyung Eun (Department of Animal and Food Biotechnology, Seoul National University) ;
  • Lee, Mooha (School of Agriculture, Arsi University)
  • 설국환 (농촌진흥청 국립축산과학원) ;
  • 김기현 (농촌진흥청 국립축산과학원) ;
  • 김영화 (농촌진흥청 국립축산과학원) ;
  • 염경은 (서울대학교 식품동물생명공학부) ;
  • 이무하 (알시대학교)
  • Received : 2014.07.31
  • Accepted : 2014.11.04
  • Published : 2014.12.31
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Abstract

This study was carried out to determine the effect of storage condition, such as temperature or relative humidity (RH) in home-style refrigerator, on the change of quality traits and storage characteristics of Hanwoo M. longissimus to find out the condition for prolongation of shelf-life with maintaining the meat quality for consumers. Samples were sliced in $1{\pm}0.2cm$ thickness, and packed in foamed polystyrene tray with linear low-density polyethylene (LLD-PE) film to simulate the pre-packed Hanwoo loin sold in retail market, then stored in home-style refrigerator ($5^{\circ}C$/17% RH, Control), and chambers of $5^{\circ}C$/55% RH (T1), $5^{\circ}C$/85% RH (T2), and $-1^{\circ}C$/99% RH (T3), respectively. Quality traits (color, pH, water holding capacity, shear force and grilling loss) and storage characteristics (thiobarbituric acid reactive substances, volatile basic nitrogen and total microbes) were measured at 1, 4, 7, 14 and 21 days after storage. Lightness of Hanwoo loin stored in T1, T2 and T3 were higher than that of control until 14 days of storage, however at the end of storage (21 days) control showed significantly higher than other treatments (p<0.05). Redness and Yellowness of Hanwoo loin samples stored in T1 and T3 were significantly higher than others during all storage period (p<0.05). The water holding capacity (WHC) of control was significantly higher than others until 14 days of storage (p<0.05), however, Hanwoo loin stored in T2 was the highest ($63.64{\pm}7.62kg/cm^2$) at 21 days of storage. Hanwoo loin stored in T1 showed significantly lower shear force than others during all storage period (p<0.05). There was no consistent tendency in pH and grilling loss during storage in all treatments. Hanwoo loin stored in T1 showed lower TBARS value than others during storage period, however there was a rapid increase to $0.34{\pm}0.27mg$ malonaldehyde/kg meat at 21 days of storage. And, all the treated samples (from T1 to T3) showed significantly lower VBN values at 21 days of storage (p<0.05). The population of total aerobic microbes were significantly increased in all treatments as storage period increasing, and the population of T3 ($2.28{\pm}0.57logCFU/g$) was the lowest at 21 days of storage (p<0.05). From those results, it could be predicted the better storage condition to maintain the meat quality and prolong the shelf-life of Hanwoo loin by lowering the temperature and adjusting the humidity about 55%.

본 연구에서는 포장 한우 등심의 저장 시 각각 다른 저장고 내 온도와 상대습도가 저장 기간별 이화학적 품질과 저장성에 미치는 영향을 살펴보았다. 한우 등심의 냉장저장시 저장온도와 상대습도는 육색을 포함한 식육의 품질특성에 영향을 미치며, 식육의 지방산패와 미생물 증식속도에도 영향을 미치는 것으로 나타났다. 본 연구의 결과 포장된 한우육의 저장 시 온도를 낮추며 상대습도를 55% 수준으로 유지하는 것이 품질을 유지하면서 저장기간을 연장하는데 도움이 되는 것으로 사료되며, 추후 온 습도 뿐만 아니라 저장고 내의 여러 조건들의 조절을 통하여 소비자가 고품질의 한우육을 장기간 보관하며 섭취할 수 있는 방안을 제시하는 것이 향후 한우 소비의 증진을 위해 필요할 것으로 사료된다.

Keywords

References

  1. A.O.A.C. 2000. American Official Analytical Chemists. 16th ed, Association of Official Analytical Chemists, Washington DC.
  2. Ayres, JC. 1960. Temperature relationships and some other characteristics of the microbial flora developing on refrigerated beef. Food Research 25:1-8. https://doi.org/10.1111/j.1365-2621.1960.tb17930.x
  3. Batifoulier, F, Mercier, Y, Gatellier, P, Renerre, M. 2002. Influence of vitamin E on lipid and protein oxidation induced by H2O2-activated MetMb in microsomal membranes from turkey muscle. Meat Science 61:389-395. https://doi.org/10.1016/S0309-1740(01)00209-1
  4. Bendall, JR, Restall, DJ. 1983. The cooking of single myofibres, small myofibre bundles and muscle strips from beef M. psoas and M. sternomandibularis muscles at varying heating rates and temperatures. Meat Science 8:93-117. https://doi.org/10.1016/0309-1740(83)90009-8
  5. Boaky, K, Mittal, GS. 1993. Changes in pH and water holding properties of Longissimus dorsi muscle during beef ageing. Meat Science 34:335-349. https://doi.org/10.1016/0309-1740(93)90082-S
  6. Bouton, PE, Carrol, FD, Fisher, AL, Harris, PV. Shothose, WR. 1983. Influence of pH and fiber contraction state up on factors affecting the tenderness of bovine muscle. J. Food Science 38:404.
  7. Brewer, MS, Harbers, CAZ. 1992. TBA values, sensory characteristics, and volatiles in ground pork during long-term frozen storage : Effect of packaging. Journal of Food Science 57(3):558-563. https://doi.org/10.1111/j.1365-2621.1992.tb08042.x
  8. Briskey, EJ. 1964. Etiological status and associated in studies of pale, soft, exudative porcine musculature. Advances in Food Research 13:89-105. https://doi.org/10.1016/S0065-2628(08)60100-7
  9. Byun, MW, Kwon, JH, Cho, HO, Lee, MK, Kim, JG. 1985. Physicochemical changes of gamma-irradiated chicken. Korean Journal of Food Science and Technology 17:186-191.
  10. Demeyer, D, Hoozee, J, Mesdom H. 1974. Specificity of lipolysis during dry sausage ripening. Journal of Food Science 39(2):293-296. https://doi.org/10.1111/j.1365-2621.1974.tb02878.x
  11. Dutson, TR. 1983. Relationship of pH and temperature to disruption of specific muscle proteins and activity of liposomal proteases. Jounal of Food Biochemistry 7:233-245.
  12. Gill, CO. 1982. Microbial interaction with meats, in Meat Microbiology, (ed. MH. Brown). Applied Science, London. 225-264.
  13. Grau, R. and Hamm, R. (1953) Eine einfache methode zur bestimmung der wasserbindung in muskel. Naturwissenschaften 40:29-30. https://doi.org/10.1007/BF00595734
  14. Grouber, SL, Tatum, JD, Scanga, JA, Chapman, PL, Smith, GC, and Belk, KE/ 2006. Effeects of postmortem aging and USDA quality grade on Warner-Blatzler shear force values of seventeen individual beef muscles. Journal of Animal Science 84:3387-3396. https://doi.org/10.2527/jas.2006-194
  15. Guignot, F, Tourraille, C, Ouali, A, Renerre, M, Monin, G. 1994. Relationships between post-mortem pH changes and some traits of sensory quality in veal. Meat Science 37:315-325. https://doi.org/10.1016/0309-1740(94)90049-3
  16. Huff, EJ. Jr. Farrish, FC. 1993. Bovine longissimus muscle tenderness as affected by postmortem ageing time, age and sex. Journal of Food Science 58:713-716. https://doi.org/10.1111/j.1365-2621.1993.tb09341.x
  17. Hwangbo, S, Rhim, TJ, Koh KC. 2001. Identification of species-specific components between Hanwoo and Aberdeen angus meat. Korean Journal of Animal Science 46:941-948.
  18. Joo, ST, Lee, HK, Kang, GH, Shin, CW, Yang, HS, Moon, SS, Lee, JI, Kim, YH, Park, GB. 2002. Effects of submersion aging in chilled water on tenderness and microbial growth of vacuum-packed Hanwoo Meat. Korean Journal for Food Science of Animal Resources 22(3):228-233.
  19. Kanner, J. 1994. Oxidative process in meat and meat products: Quality implications. Meat Science 36:169-189. https://doi.org/10.1016/0309-1740(94)90040-X
  20. Kim, BC, Joo, ST, Park, GB, Sung, SK, Lee, M, Lee, SK, Jung, MS, Choi, YI. 1998. Science of muscle foods pp. 71-91. Seonjin publishers.
  21. Kim, CJ. 1994. Studies on the cold and frozen storage for the production of high quality meat of Korean Native Cattle I. Effects of cold and frozen storage on the meat color, sarcomere length and tenderness in Korean Native Cattle. Korean Journal for Food Science of Animal Resources 14:151-154.
  22. Kim, CJ. Lee, ES. 2003. Effects of quality grade on the chemical, physical and sensory characteristics of Hanwoo (Korean native cattle) beef. Meat Science 63:397-405. https://doi.org/10.1016/S0309-1740(02)00099-2
  23. Kim, IS, Lee, SO, Byun, JS, Kang, SN, Min, JS, Lee, M. 2000. Physicochemical, microbiological, and sensory characteristics of frozen loins of Hanwoo and importe. Korean Journal of Animal Science 42:117-124.
  24. Kim, IS, Lee, SO, Lee, JM, Kim, JH, Kim, YG. 2000. Physicochemical, microbiological, and sensory characteristics of chilled chuck rolls of Hanwoo. Korean Journal of Animal Science 42:109-116.
  25. Kim, KH. Kim, YS. Lee, YK. Baik, MG. 2000. Postmortem muscle glycolysis and meat quality characteristics of intact male Korean native (Hanwoo) cattle. Meat Science 55:47-52. https://doi.org/10.1016/S0309-1740(99)00124-2
  26. Ledward, DA, Macfarlane, JJ. 1971. Some observations on myoglobin and lipid oxidatin in frozen beef. Journal of Food Science 36:987-990.
  27. Lee, JM, Kim, TW, Kim, JH, Cho, SH, Seong, PN, Jung, MO, Cho, YM, Park, BY, Kim, DH. 2009. Comparison of chemical, physical and sensory traits of Longissimus Lumborum Hanwoo Beef and Australian Wagyu Beef. Korean Journal for Food Science of Animal Resources 29:91-98. https://doi.org/10.5851/kosfa.2009.29.1.91
  28. Maher, SC, Mullen, AM, Buckley, DJ, Kerry, JP, Moloney, AP. 2005. The influence of biochemical differences on the variation in tenderness of M. longissimus dorsi of Belgian Blue steers managed homogenously pre and post-slaughter. Meat Science 69:215-224. https://doi.org/10.1016/j.meatsci.2004.06.024
  29. Miller, AJ, Ackerman, SA, Palumbo, SA. 1980. Effect of frozen storage on functionality of meat for processing. Journal of Food Science 45:1466-1471. https://doi.org/10.1111/j.1365-2621.1980.tb07541.x
  30. Moon, SS, Yang, HS, Park, GB, Joo, ST. 2006. The relationship of physiological maturity and marbling judged according to Korean grading system to meat quality traits of Hanwoo beef females. Meat Science 74:516-521. https://doi.org/10.1016/j.meatsci.2006.04.027
  31. Park, GB, Moon SS, Ko, YD, Ja, JK, Lee, JG, Chang, HH, Joo, ST. 2002. Influence of slaughter weight and sex on yield and quality grades of Hanwoo (Korean native cattle) carcasses. Journal of Animal Science 80:129-136.
  32. Rhee, MS, Kim, BC. 2001. Effect of low voltage clectrical stimulation and temperature conditioning on postmortem changes in glycolysis and calpains activities of Korean native cattle (Hanwoo). Meat Science 58:231-237. https://doi.org/10.1016/S0309-1740(00)00155-8
  33. SAS/STAT. 2012. SAS user's guide. SAS Institute Inc., Cary, NC.
  34. Shackelford, SD, Wheeler, TL, Koohmaraie, M. 1999. Tenderness classification of beef: II. Design and analysis of a system to measure beef longissimus shear force under commercial processing conditions. Journal of Animal Science 77:1474-1481.
  35. Turner, E.W.; Paynter, W.D.; Montie, E.J.; Bessert, M.W.; Struck, G.M. and Olson, F.C. (1954): Use of the 2-Thiobarbituric acid reagent to measure rancidity in frozen pork. Food Technology 8:326-330.
  36. Watanabe, A, Daly, CC, Devine, CE. 1996. The effects of the ultimate pH of meat on tenderness changes during aging. Meat Science 42:67-78. https://doi.org/10.1016/0309-1740(95)00012-7
  37. Weatherly, BH, Lorenzen, CL, Savell, JW. 1998. Determining optimal aging times for beef subprimals. Journal of Animal Science 76(Suppl. 1):598. (Abstract)
  38. Winger, RJ, Fennema, O. 1976. Tenderness and water holding properties of beef muscle as influenced by freezing and subsequent storage at -3 or $15^{\circ}C$. Journal of Food Science 41:1433-1438. https://doi.org/10.1111/j.1365-2621.1976.tb01189.x
  39. Witte, VC, Krause, GF, Bailey, ME. 1970, A new extraction method for determining 2-thiobarbituric acid values of pork and beef during storage. Journal of Food Science 35:582-585. https://doi.org/10.1111/j.1365-2621.1970.tb04815.x
  40. Yamamoto, K, Samejima, K. 1977. A comparative study of the change in hen pectoral muscle during storage at $4^{\circ}C$ and $-20^{\circ}C$. Journal of Food Science 42:1642-1645. https://doi.org/10.1111/j.1365-2621.1977.tb08446.x
  41. Yang, SY, Lim, SD, Jeon, KH, Nam, KB, Kwon, SA, Park, JE. 2007. Comparison of vitamin A, E, and cholesterol contents and the sensory properties of chilled Hanwoo and Australian Beef. Korean Journal for Food Science of Animal Resources 27:262-266. https://doi.org/10.5851/kosfa.2007.27.3.262
  42. Yin, M. Faustman, C. 1993. a-Tocopherol and ascorbate delay oxymyoglobin and phospholipid oxidation in vitro. Journal of Food Science 58:1273-1276. https://doi.org/10.1111/j.1365-2621.1993.tb06164.x
  43. 高坂和久. 1975. 肉製品の 鮮度保侍と測定. 食品工業 18:257.

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