Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Protective Effects of Green Tea Seed Extract against UVB-irradiated Human Skin Fibroblasts

인체 피부 섬유아세포에서 자외선 조사에 대한 녹차나무 씨 추출물의 보호효과

  • Received : 2013.09.23
  • Accepted : 2013.11.18
  • Published : 2014.01.31
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Abstract

In this study, we investigated the protective effects of green tea seed extract (GSE) against UVB-induced skin damage in human skin fibroblasts. GSE was first analyzed for antioxidant activity using 1,1-diphenyl-2picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical scavenging assays. Treatment of UV-irradiated fibroblast with GSE at 10~50 ${\mu}g/mL$ significantly increased DPPH and ABTS radical scavenging activities in a dose-dependent manner. GSE treatment inhibited matrix metalloproteinase (MMP-1, MMP-3, and MMP-9) expression and MMP-1 secretion caused by UVB irradiation. Moreover, treatment with GSE significantly increased type-1 collagen expression and production. We next examined levels of antioxidative enzymes (SOD, catalase, and GPx). Reduced antioxidative enzyme activities caused by UVB irradiation were recovered by treatment with GSE at 30 ${\mu}g/mL$ and 50 ${\mu}g/mL$. In conclusion, these results show that GSE has protective effects against UVB-induced skin damage in human skin fibroblasts by regulating antioxidative defense systems and MMP expression.

본 연구는 아직 기능성 입증이 미비한 녹차나무 씨 추출물을 사용하여 자외선을 조사한 인체 피부 섬유아세포에서 항산화 효능과 MMPs 및 collagen의 변화를 살펴봄으로써 광노화 억제 효능을 평가하였다. 녹차나무 씨 추출물 농도별 라디칼 소거능을 살펴보기 위하여 ABTS와 DPPH 라디칼 소거능을 측정한 결과 녹차나무 씨 추출물 농도 의존적으로 소거능을 증가시켰다. 25 $mJ/cm^2$ 자외선을 조사한 인체 피부 섬유아세포에서 녹차나무 씨 추출물의 농도별(10 ${\mu}g/mL$, 30 ${\mu}g/mL$, 50 ${\mu}g/mL$) 처리가 미치는 영향을 살펴보았다. 항산화 효소(SOD, GPx, catalase) 활성의 변화를 측정한 결과에서 자외선 조사에 의하여 감소한 활성을 녹차나무 씨 추출물의 처리가 유의적으로 증가시켰음을 확인하였다. 또한 녹차나무 씨 추출물의 처리는 MMP-1의 합성을 감소시키고 collagen의 합성을 증가시켰으며, MMP-1, MMP-3, MMP-9의 mRNA 발현을 감소시키고 type-1 collagen의 mRNA 발현을 증가시켰다. 따라서 녹차나무 씨 추출물은 항산화 활성을 지녔으며 자외선 조사에 의한 활성산소종으로부터 보호하여 MMPs 발현을 감소시키고 collagen 분해를 억제시켜 피부 노화억제에 긍정적인 영향을 미쳤으며, 이러한 기능성 입증으로 녹차나무의 활용 증가를 기대할 수 있다.

Keywords

References

  1. Fisher GJ, Kang S, Varani J, Bata-Csorgo Z, Wan Y, Datta S, Voorhees JJ. 2002. Mechanisms of photoaging and chronological skin aging. Arch Dermatol 138: 1462-1470.
  2. Chung JH. 2003. Photoaging in asians. Photodermatol Photoimmunol Photomed 19: 109-121. https://doi.org/10.1034/j.1600-0781.2003.00027.x
  3. Rittie L, Fisher GJ. 2002. UV-light-induced signal cascades and skin aging. Ageing Res Rev 1: 705-720. https://doi.org/10.1016/S1568-1637(02)00024-7
  4. Seite S, Zucchi H, Septier D, Igondjo-Tchen S, Senni K, Godeau G. 2006. Elastin changes during chronological and photo‐ageing: the important role of lysozyme. J Eur Acad Dermatol Venereol 20: 980-987.
  5. Fisher GJ, Wang ZQ, Datta SC, Varani J, Kang S, Voorhees JJ. 1997. Pathophysiology of premature skin aging induced by ultraviolet light. N Engl J Med 337: 1419-1428. https://doi.org/10.1056/NEJM199711133372003
  6. Castanet J, Ortonne JP. 1997. Pigmentary changes in aged and photoaged skin. Arch Dermatol 133: 1296-1299. https://doi.org/10.1001/archderm.1997.03890460120015
  7. Fisher GJ, Datta SC, Talwar HS, Wang ZQ, Varani J, Kang S, Voorhees JJ. 1996. Molecular basis of sun-induced premature skin ageing and retinoid antagonism. Nature 379:335-339. https://doi.org/10.1038/379335a0
  8. Kovacs D, Raffa S, Flori E, Aspite N, Briganti S, Cardinali G, Torrisi MR, Picardo M. 2009. Keratinocyte growth factor down-regulates intracellular ROS production induced by UVB. J Dermatol Sci 54: 106-113. https://doi.org/10.1016/j.jdermsci.2009.01.005
  9. Halliwell B, Gutteridge JM. 1984. Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem J 219: 1-14.
  10. Shephard P, Martin G, Smola-Hess S, Brunner G, Krieg T, Smola H. 2004. Myofibroblast differentiation is induced in keratinocytefibroblast co-cultures and is antagonistically regulated by endogenous transforming growth factor-${\beta}$ and interleukin-1. Am J Pathol 164: 2055-2066. https://doi.org/10.1016/S0002-9440(10)63764-9
  11. Brenneisen P, Sies H, Scharffetter-Kochanek K. 2002. Ultraviolet-B irradiation and matrix metalloproteinases: from induction via signaling to initial events. Ann NY Acad Sci 973: 31-43. https://doi.org/10.1111/j.1749-6632.2002.tb04602.x
  12. Ito S, Itoga K, Yamato M, Akamatsu H, Okano T. 2010. The co-application effects of fullerene and ascorbic acid on UV-B irradiated mouse skin. Toxicology 267: 27-38. https://doi.org/10.1016/j.tox.2009.09.015
  13. Darr D, Fridovich I. 1994. Free radicals in cutaneous biology. J Invest Dermatol 102: 671-675. https://doi.org/10.1111/1523-1747.ep12374036
  14. Masteikova R, Muselik J, Bernatoniene J, Majiene D, Savickas A, Malinauskas F, Bernatoniene R, Peciura R, Chalupova Z, Dvorackova K. 2008. Antioxidant activity of tinctures prepared from hawthorn fruits and motherwort herb. Ceska Slov Farm 57: 35-38.
  15. Ruch RJ, Cheng SJ, Klaunig JE. 1989. Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis 10: 1003-1008. https://doi.org/10.1093/carcin/10.6.1003
  16. Zheng G, Sayama K, Okubo T, Juneja LR, Oguni I. 2004. Anti-obesity effects of three major components of green tea, catechins, caffeine and theanine, in mice. In Vivo 18: 55-62.
  17. Peng G, Dixon DA, Muga SJ, Smith TJ, Wargovich MJ. 2006. Green tea polyphenol (-)-epigallocatechin-3-gallate inhibits cyclooxygenase-2 expression in colon carcinogenesis. Mol Carcinog 45: 309-319. https://doi.org/10.1002/mc.20166
  18. Park JS, Rho HS, Kim DH, Chang IS. 2006. Enzyme preparation of kaempferol from green tea seed and its antioxidant activity. J Agric Food Chem 54: 2951-2956. https://doi.org/10.1021/jf052900a
  19. Kim NH, Choi SK, Kim SJ, Moon PD, Lim HS, Choi IY, Na HJ, An HJ, Myung NY, Jeong HJ, Um JY, Hong SH, Kim HM. 2008. Green tea seed oil reduces weight gain in C57BL/6J mice and influences adipocyte differentiation by suppressing peroxisome proliferator-activated receptorgamma. Pflugers Arch 457: 293-302. https://doi.org/10.1007/s00424-008-0537-y
  20. Yoon WH, Choi JH, Lee KH, Kim CH. 2005. Antimicrobial and antitumor activities of seed extracts of Camellia sinensis L. Korean J Food Sci Technol 37: 108-112.
  21. Rah HH, Baik SO, Han SB, Bock JY. 1992. Chemical compositions of the seed of Korean green tea plant (Camellia sinecis L.). J Korean Agric Chem Soc 35: 272-275.
  22. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26: 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  23. Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200. https://doi.org/10.1038/1811199a0
  24. Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65: 55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  25. Sanchez-Moreno C. 2002. Methods used to evaluate the free radical scavenging activity in foods and biological systems. Food Sci Technol Int 8: 121-137. https://doi.org/10.1177/1082013202008003770
  26. Cano A, Acosta M, Arnao MB. 2000. A method to measure antioxidant activity in organic media: application to lipophilic vitamins. Redox Rep 5: 365-370. https://doi.org/10.1179/135100000101535933
  27. Arnao MB, Cano A, Acosta M. 2001. The hydrophilic and lipophilic contribution to total antioxidant activity. Food Chem 73: 239-244. https://doi.org/10.1016/S0308-8146(00)00324-1
  28. Gutiérrez RMP, Mitchell S, Solis RV. 2008. Psidium guajava: a review of its traditional uses, phytochemistry and pharmacology. J Ethnopharmacol 117: 1-27. https://doi.org/10.1016/j.jep.2008.01.025
  29. Apel K, Hirt H. 2004. Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 55: 373-399. https://doi.org/10.1146/annurev.arplant.55.031903.141701
  30. Michiels C, Raes M, Toussaint O, Remacle J. 1994. Importance of Se-glutathione peroxidase, catalase, and Cu/Zn-SOD for cell survival against oxidative stress. Free Radic Biol Med 17: 235-248. https://doi.org/10.1016/0891-5849(94)90079-5
  31. Deisseroth A, Dounce AL. 1970. Catalase physical and chemical properties, mechanism of catalysis and physiological role. Physiol Rev 50: 3-24.
  32. Talwar HS, Griffiths CEM, Fisher GJ, Hamilton TA, Voorhees JJ. 1995. Reduced type I and type III procollagens in photodamaged adult human skin. J Invest Dermatol 105:285-290. https://doi.org/10.1111/1523-1747.ep12318471
  33. Fisher GJ, Voorhees JJ. 1998. Molecular mechanisms of photoaging and its prevention by retinoic acid: ultraviolet irradiation induces MAP kinase signal transduction cascades that induce Ap-1-regulated matrix metalloproteinases that degrade human skin in vivo. J Investig Dermatol Symp Proc 3: 61-68.

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