Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Anticancer Effects of Cultivated Orostachys japonicus on Human Prostate Cancer Cells

인체 전립선 암세포에서 재배 와송의 항암효과

  • Won, Yeong Seon (Dept. Food and Nutrition, Sunchon National University) ;
  • Lee, Ju Hye (Research Institute of Basic Science, Sunchon National University) ;
  • Kwon, Soon Jae (Dept. of Food Science and Technology, Kyungpook National University) ;
  • Ahn, Dong Uk (Dept. of Animal Science, Iowa State University) ;
  • Shin, Dong Young (Dept. of Development in Resource Plants, Sunchon National University) ;
  • Seo, Kwon Il (Dept. Food and Nutrition, Sunchon National University)
  • 원영선 (순천대학교 식품영양학과) ;
  • 이주혜 (순천대학교 기초과학연구소) ;
  • 권순재 (경북대학교 식품공학과) ;
  • 안동욱 (아이오와주립대학교 동물과학과) ;
  • 신동영 (순천대학교 자원식물개발학과) ;
  • 서권일 (순천대학교 식품영양학과)
  • Received : 2013.09.13
  • Accepted : 2013.12.19
  • Published : 2014.01.31
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Abstract

This study was performed to determine the anticancer effects of cultivated Orostachys japonicus (COJ) and wild Orostachys japonicus (WOJ) on primary human prostate cancer cells (RC-58T/h/SA#4 cells). The morphology of cells treated with COJ and WOJ was distorted to shrunken cell masses. In addition, cell death induced by COJ and WOJ was associated with increased population of cells in sub-G1 phase as well as the formation of apoptotic bodies and nuclear condensation. COD and WOJ markedly reduced the number of viable prostate cancer cells in a dose-dependent manner, and cell numbers were lower than control cells. COJ and WOJ also inhibited increases in cell proliferation induced by environmental hormones such as dioxin and bisphenol A in charcoal-treated FBS (cFBS) medium. COJ and WOJ methanol extracts at the tested concentrations (150, 300, and 600 ${\mu}g/mL$) also dose-dependently inhibited cell proliferation induced by environmental hormones. These results indicate that COJ and WOJ exert anticancer effects on primary human prostate cancer cells.

재배 와송과 천연 와송의 항암효과 및 apoptosis 유도효과를 입증하기 위하여 RC-58T/h/SA#4 primary 인체 전립선 암세포를 이용하여 다양한 실험을 실시하였다. 그 결과 재배 와송과 천연 와송은 전립선암세포에서 농도 의존적으로 높은 암세포 증식 억제효과를 나타내었으며, 대조군과 비교하여 와송 메탄올 추출물 처리 시 세포의 형태가 수축되고 세포의 수가 감소한 것을 형태학적 관찰을 통해 확인할 수 있었다. 또한 와송 메탄올 추출물의 처리에 따른 RC-58T/h/SA#4 세포에서의 apoptosis 유도효과는 sub-G1기의 함량 증가와 hoechst 염색을 통한 apoptotic bodies 형성 및 핵형태 변화를 관찰을 통해 확인할 수 있었다. 한편 재배 와송과 천연 와송은 dioxin과 bisphenol A와 같은 환경호르몬 처리에 의해 비정상적으로 증가된 전립선암세포의 성장을 억제하는 것으로 나타났다. 본 연구 결과, 재배 와송과 천연와송은 RC-58T/h/SA#4 전립선암 세포에서 유사한 암세포 성장 억제효과와 apoptosis 유도효과를 가지고 있음을 확인하였으며, 환경호르몬에 의해 유발될 수 있는 암에 대해서도 유사한 보호 효과를 가지고 있음을 증명하였다.

Keywords

References

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