Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Anti-obesity Effects of Extracts from Young Akebia quinata D. Leaves

어린 으름잎 추출물(Akebia quinata D. Leaves)의 항비만 효과

  • Jeon, Yongseop (Div. of Food and Nutrition, Chonnam National University) ;
  • You, Yanghee (Research Institute for Human Ecology, Chonnam National University) ;
  • Jun, Woojin (Div. of Food and Nutrition, Chonnam National University)
  • 전용섭 (전남대학교 식품영양과학부) ;
  • 유양희 (전남대학교 생활과학연구소) ;
  • 전우진 (전남대학교 식품영양과학부)
  • Received : 2013.12.02
  • Accepted : 2014.01.13
  • Published : 2014.02.28
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Abstract

We investigated the in vitro and in vivo anti-obesity effects of extracts from young Akebia quinata D. leaves, including hot water (AQH) and 80% ethanol (AQE) extracts. The inhibitory effects of AQH and AQE on lipid accumulation in 3T3-L1 cells were examined by Oil Red O staining. Compared to control, lipid accumulation was significantly reduced by 18.3% with the treatment upon AQE at a concentration of $5{\mu}g/mL$. The levels of intracellular triglycerides and free glycerol were also reduced by 52.8% and 9.1% at the same concentration of AQE. The in vivo anti-obesity effect of AQE was evaluated in terms of body and white adipose tissue weights in ICR mice. Experimental groups were divided into the following five groups: normal diet (ND), high fat diet (HFD), high fat diet with 60 mg/kg/day of Orlistat (HFD-RF), high fat diet with 200 mg/kg/day of AQE (HFD-AL), and high fat diet with 600 mg/kg/day of AQE (HFD-AH). Feeding of HFD for eight weeks resulted in significant increases in body weight as well as weight gain compared to the ND group. HFD-AH group showed reduced body weight, weight gain, epididymal white adipose tissue weight, and perirenal white adipose weight as compared to the HFD group. These results indicate that AQE supplementation might have beneficial effects on anti-obesity by inhibiting lipid accumulation.

본 연구에서 어린 으름잎으로부터 추출된 열수추출물인 AQH와 80% 주정추출물인 AQE를 대상으로 3T3-L1 전지방세포의 지방세포 분화과정 중에 처리한 후, Oil Red O 염색법에 의한 lipid accumulation, intracellular 중성지방 함량을 평가하고 free glycerol release 함량을 측정하여 지방세포형성 억제능을 확인하며, ICR 마우스를 대상으로 고지방식이 섭취 하에 어린 으름잎 추출물의 경구투여에 따른 체중과 백색지방에 작용하는 효과를 평가하여 어린 으름잎 추출물의 항비만 효과를 밝히고자 하였다. 3T3-L1 지방세포 분화과정 중 AQH 및 AQE 추출물을 처리한 결과 AQE 처리군에서만 유의적 감소를 나타내었다. Intracellular 중성지방 함량의 경우도 AQE 처리군에서 유의적으로 감소되었다. 지방세포 분화 중 배양액으로 유출되는 free glycerol 함량을 평가한 결과에서도 AQE 처리군의 유의적 감소를 확인할 수 있었다. 이상의 결과를 통해 AQE는 3T3-L1 지방세포 분화과정 중에 지방구 생성 억제 및 지방세포의 지방세포 생성 억제효과가 있는 것으로 판명되었다. 마우스에서 8주간 고지방식이 하에 600 mg/kg/day의 AQE를 경구투여 하였을 때 고지방식이군과 비교하여 체중 및 체중증가량이 통계적으로 유의하게 감소하였고, 부정소 주위 및 신장 주위 백색지방의 감소가 나타났다. AQE 투여에 의한 체중 감소 및 부정소 주위 백색지방량의 감소는 양성대조군으로 사용한 Orlistat의 경구투여 결과와 유사하였다. 이상의 결과로 세포수준에서의 지방세포 분화 억제, 지방구 생성 억제 작용과 실험동물에서 체중증가량 및 체지방의 감소 작용을 갖는 AQE는 향후 항비만 효과를 갖는 소재로 유용하게 사용될 수 있을 것으로 사료된다.

Keywords

References

  1. Spiegelman BM, Flier S. 1996. Adipogenesis and obesity; rounding out the big picture. Cell 87: 377-389. https://doi.org/10.1016/S0092-8674(00)81359-8
  2. Kopelman PG. 2000. Obesity as a medical problem. Nature 404: 635-643.
  3. Visscher TL. 2001. The public health impact of obesity. Annu Rev Publ Health 22: 355-375. https://doi.org/10.1146/annurev.publhealth.22.1.355
  4. Frayn KN, Karpe F, Fielding BA, Macdonald IA, Coppack SW. 2003. Integrative physiology of human adipose tissue. Int J Obes Relat Metab Disord 27: 875-888. https://doi.org/10.1038/sj.ijo.0802326
  5. Kopelman PG. 2000. Obesity as a medical problem. Nature 404: 635-643.
  6. Green H, Meuth M. 1974. An established pre-adipose cell line and its differentiation in culture. Cell 3: 127-133. https://doi.org/10.1016/0092-8674(74)90116-0
  7. Green H, Kehinde O. 1973. Sublines of mouse 3T3 cells that accumulate lipid. Cell 1: 113-116.
  8. Aihaud G, Grimaldu P, Negrel R. 1992. Cellular and molecular aspects of adipose tissue development. Annu Rev Nutr 12: 207-233. https://doi.org/10.1146/annurev.nu.12.070192.001231
  9. Boney CM, Staats BM, Atiles AD, Dercole AJ. 1994. Expression on insulin-like growth factor-I(IGF-I) and IGFbinding proteins during adipogenesis. Endocrinology 135: 1863-1868.
  10. Darlington GJ, Ross SE, MacDougald OA. 1998. The role of C/EBP genes in adipocyte differentiation. J Biol Chem 273: 30057-30060. https://doi.org/10.1074/jbc.273.46.30057
  11. Rosen ED, Hsu CH, Wang X, Sakai X, Freeman MW, Gonzalez FJ, Spiegelman BM. 2001. C/EBP$\alpha$ induces adipogenesis through PPAR: a unified pathway. Genes Dev 16: 22-26.
  12. Naowaboot J, Chung CH, Pannangpetch P, Choi R, Kim BH, Lee MY, Kukongviriyapan U. 2012. Mulberry leaf extract increases adiponectin in murine 3T3-L1 adipocytes. Nutr Res 32: 39-44. https://doi.org/10.1016/j.nutres.2011.12.003
  13. Sato M, Hiragun A, Mitsui H. 1980. Preadipocytes possess cellular retinoid binding proteins and their differentiation is inhibited by retinoids. Biochem Biophys Res Commun 95: 1839-1845. https://doi.org/10.1016/S0006-291X(80)80113-6
  14. Kawada T, Aoki N, Kamei Y, Maeshige K, Nishiu S, Sugimoto E. 1990. Comparative investigation of vitamins and their analogues on terminal differentiation from preadipocytes to adipocytes of 3T3-L1 cells. Comp Biochem Physiol 96: 323-326. https://doi.org/10.1016/0300-9629(90)90699-S
  15. Cno M, Aratani Y, Kitagawa I, Kitagawa Y. 1990. Ascorbic acid phosphate stimulate type IV collagen synthesis and accelerate adipose conversion of 3T3-L1 cells. Exp Cell Res 187: 309-314. https://doi.org/10.1016/0014-4827(90)90096-S
  16. Harrison SA, BuxtaonJM, Clancy BM, Czech MP. 1991. Evidence that erythroid-type glucose transporter intrinsic activity is modulated by cadmium treatment of mouse 3T3-L1 cells. J Biol Chem 266: 19438-19449.
  17. Park HS. 2001. Pharmacological therapy of obesity. Kor J Soc Study Obes 10: 118-127.
  18. Ballinger A, Peikin SR. 2002. Orlistat: its current status as an anti-obesity drug. Eur J Pharmacol 440: 109-117. https://doi.org/10.1016/S0014-2999(02)01422-X
  19. Zacour AC, Sliva ME, Cecon PR, Bambirra EA, Vieira EC. 1992. Effect of dietary chitin on cholesterol absorption and metabolism in rats. J Nutr Sci Vitaminol 38: 609-613. https://doi.org/10.3177/jnsv.38.609
  20. Kim YJ, Kim BH, Lee SY, Kim MS, Park CS, Rhee MS, Lee KH, Kim DS. 2006. Screening of medicinal plants for development of functional food ingredients with antiobesity. J Korean Soc Appl Biol Chem 49: 221-226.
  21. Kim YS, Byun SH, Kim SC, Kum M, Cho EH. 2000. Effect on cure and prevention of an obesity. Kor J Herbology 15: 37-43.
  22. Jeon JR, Kim JY, Lee KM, Cho DH. 2005. Anti-obese effects of mixture contained pine needle, black tea and green tea extracts. J Korean Soc Appl Biol Chem 48: 375-381.
  23. Choi J, Jung HJ, Lee KT, Park HJ. 2005. Antinociceptive and anti-inflammatory effects of the saponin and obtained from the stem of Akebia quinata. J Med Food 8: 78-85. https://doi.org/10.1089/jmf.2005.8.78
  24. Jung HJ, Lee CO, Lee KT, Park HJ. 2004. Structure activity relationship of oleanane disaccharides isolated from Akebia quinata versus cytotoxicity against cancer cells and NO inhibition. Biol Pharm Bull 27: 744-747. https://doi.org/10.1248/bpb.27.744
  25. Higuchi R, Kawasaki T. 1976. Pericarp saponins of Akebia quinata Decne. I. Glycosides of hederagenin and oleanolic acid. Chem Pharm Bull 24: 1021-1032. https://doi.org/10.1248/cpb.24.1021
  26. Ikuta A, Itokawa H. 1988. A triterpene from Akebia quinata callus tissue. Phytochemistry 27: 3809-3810. https://doi.org/10.1016/0031-9422(88)83022-X
  27. Rim AR, Kim SJ, Jeon KI, Park EJ, Park HR, Lee SC. 2006. Antioxidant activity of extracts from Akebia quinata Decne. J Food Sci Nutr 11: 84-87. https://doi.org/10.3746/jfn.2006.11.1.084
  28. You YH, Jun WJ. 2012. Effects of fractions from Benincasa hispida on inhibition of adipogenesis in 3T3-L1 preadipocytes. J Korean Soc Food Sci Nutr 41: 895-900. https://doi.org/10.3746/jkfn.2012.41.7.895
  29. Yoon BR, Lee YJ, Kim SG, Jang JY, Lee HK, Rhee SK, Hong HD, Choi HS. 2012. Antioxidant effect of hot water and ethanol extracts from Cheonnyuncho (Opuntia humidusa) on reactive oxygen species (ROS) production in 3T3- L1 adipocytes. Korean J Food Preserv 19: 443-450. https://doi.org/10.11002/kjfp.2012.19.3.443
  30. Kim MJ, Kim HK. 2009. Perilla leaf extract inhibits 3T3-L1 preadipocytes differentiation. Food Sci Biotechnol 18: 928-931.
  31. Liu F, Kim J, Li Y, Liu X, Li J, Chen X. 2001. An extract of Lagerstroemia speciosa L has insulin-like glucose uptake stimulatory and adipocyte differentiation-inhibitory activities in 3T3-L1 cells. J Nutr 131: 2242-2247.
  32. Grundy SM. 2004. Obesity, metabolic syndrome, and cardiovascular disease. J Clin Endocrinol Metab 89: 2595-2600. https://doi.org/10.1210/jc.2004-0372
  33. Tansey JT, Sztalryd C, Hlavin EM, Kimmel AR, Londos C. 2004. The central role of perilipin a in lipid metabolism and adipocyte lipolysis. IUBMB Life 56: 379-385. https://doi.org/10.1080/15216540400009968
  34. Lee JM, Yoon HK, Lee YH, Park JJ, You YH, Jang JY, Yang JW, Jun WJ. 2010. The potential effects of ethyl acetate fraction from Curcuma longa L. on lipolysis in differentiated 3T3-L1 adipocytes. J Med Food 13: 1-7. https://doi.org/10.1089/jmf.2009.1142
  35. Hong SW, Lee JM, Choi JH, Park SE, Rhee EJ, Park CY, Oh KW, Park SW, Kim SW, Lee WY. 2012. The effect of AMPK activation on Wnt and sFRP5 in TNF-$\alpha$ induced adipocyte metabolic dysfunction in 3T3-L1 cell. Korean J Obes 21: 29-36. https://doi.org/10.7570/kjo.2012.21.1.29
  36. Seo DJ, Kim1 JM, Kim TH, Baek JM, Kim TW, Kim HS, Choe M. 2010. Anti-obesity effects of Foeniculum fructus water extract. J Korean Soc Food Sci Nutr 39: 1604-1610. https://doi.org/10.3746/jkfn.2010.39.11.1604
  37. Lee JJ, Shin HD, Lee YM, Kim AR, Lee MY. 2009. Effect of broccoli sprouts on cholesterol-lowering and anti-obesity effects in rats fed high fat diet. J Korean Soc Food Sci Nutr 38: 309-318. https://doi.org/10.3746/jkfn.2009.38.3.309
  38. Park YS, Yoon Y, Ahn HS. 2007. Platycodon grandiflorum extract represses up-regulated adipocyte fatty acid binding protein triggered by a high fat feeding in obese rats. World J Gastroenterol 13: 3493-3499. https://doi.org/10.3748/wjg.v13.i25.3493
  39. Kim HS, Kim TW, Kim DJ, Hwang HJ, Lee HJ, Choe M. 2007. Effects of natural plants supplementation on adipocyte size of the epididymal fat pads in rats. J Korean Soc Food Sci Nutr 36: 419-423. https://doi.org/10.3746/jkfn.2007.36.4.419
  40. Xue X, Xiao Y, Gong L, Guan S, Liu Y, Lu H, Qi X, Zhang Y, Li Y, Wu X, Ren J. 2008. Comparative 28-day repeated oral toxicity of Longdan Xieganwan, Akebia trifoliate (Thunb.) koidz., Akebia quinata (Thunb.) Decne. and Caulis aristolochiae manshuriensis in mice. J Ethnopharmacol 119: 87-93. https://doi.org/10.1016/j.jep.2008.05.037

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