Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Immunomodulatory Effects of Fermented Curcuma longa L. Extracts on RAW 264.7 Cells

RAW 264.7 세포에서 발효 울금 추출물의 면역조절 효과

  • 유선아 (경희대학교 의학영양학과) ;
  • 김옥경 (경희대학교 의학영양학과) ;
  • 남다은 (경희대학교 의학영양학과) ;
  • 김용재 (한국인스팜(주) 중앙연구소) ;
  • 백흠영 (한국인스팜(주) 중앙연구소) ;
  • 전우진 (전남대학교 식품영양학과) ;
  • 이정민 (경희대학교 의학영양학과)
  • Received : 2013.09.23
  • Accepted : 2013.12.17
  • Published : 2014.02.28
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Abstract

Curcuma longa L. (CL) is a well known traditional medicinal plant that is also used in curries and mustards as a coloring and flavoring agent. However, CL is not usually used as a food source due to its bitter taste. We investigated the immunomodulatory effect of CL fermented by Aspergillus oryzae (FCL) on RAW 264.7 cells. FCL was extracted with cold water (CW), hot water (HW), 20% ethanol (20% EtOH) and 80% ethanol (80% EtOH), after which its effects on phagocytic activity, tumor necrosis factor-alpha (TNF-${\alpha}$), nitric oxide (NO) production, natural killer (NK) cell activity and mRNA expression of LP-BM5 eco were investigated. Phagocytic activity was increased in HW and 20% EtOH when compared to the control. The secretion of nitric oxide (NO) from RAW 264.7 cells did not change significantly relative to the control. However, TNF-${\alpha}$ was significantly increased by the addition of FCL extracts. Moreover, FCL 20% ethanol extract showed a four fold increase in NK cell cytotoxity relative to the control group. Finally, we observed suppressed mRNA expression of LP-BM5 eco in FCL extracts, especially in the 20% ethanol extracts group. These results indicate that the FCL extracts can be used as a functional material due to their effective immunomodulating activities.

본 연구에서는 울금의 선호도 및 이용율을 높이기 위해 Aspergillus oryzae를 이용하여 발효시킨 후 용매별 추출을 통하여 얻은 발효 울금 추출물들을 사용하여 면역조절 효과를 살펴보았다. MTT assay를 이용하여 실온 추출물 $200{\mu}g/mL$, 열수 추출물 $400{\mu}g/mL$, 20% 주정 추출물 $200{\mu}g/mL$, 80% 주정 추출물 $100{\mu}g/mL$의 농도가 적정하다고 판단되어 추후 실험에 사용하도록 하였다. 면역조절 효과를 보고자 대식세포의 탐식작용 능력, nitric oxide(NO), TNF-${\alpha}$ 생성, NK 세포 활성, SC-1 세포에서 발현되는 LP-BM5 virus의 LP-BM5 eco 유전자 발현을 측정하였다. NK 세포의 활성을 측정한 결과 NK 세포는 YAC-1 세포에 대한 cytotoxity를 관찰했을 때 20% 주정 추출물이 가장 활성을 나타내는 것으로 확인하였다. 발효 울금 추출물 중 열수 추출물과 20% 주정 추출물은 대식세포를 활성화시킴으로써 탐식능력을 증가시켰다. NO 생성을 관찰했을 때 발효 울금처리에 의해 활성화된 대식세포는 효과를 나타내지 않았으나 80% 주정 추출물을 제외한 추출물에서 TNF-${\alpha}$ 생성을 정상군에 비해 유의적으로 증가시켰다. SC-1 세포에 있는 LP-BM5 eco 유전자 발현을 비교하여 바이러스 복제 억제능을 측정한 결과 모든 추출물이 유의적으로 억제되었으며, 특히 20% 주정 추출물이 가장 많이 억제하고 있는 것을 확인할 수 있다. 이러한 연구 결과로부터 발효 울금이 면역조절 효과를 가진 천연 기능성 소재로 개발에 있어 기초자료로 활용할 수 있을 것으로 기대할 수 있다.

Keywords

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