Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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The Effect of Cordycepin on the Production of Pro-inflammatory Cytokines in Mouse Peritoneal Macrophages

코디세핀이 마우스 복강 대식세포에서 전염증성 사이토카인의 생성에 미치는 영향

  • Seo, Min-Jeong (Medi-Farm Industrialization Research Center, Dong-A University) ;
  • Kang, Byoung-Won (Medi-Farm Industrialization Research Center, Dong-A University) ;
  • Kim, Min-Jeong (Medi-Farm Industrialization Research Center, Dong-A University) ;
  • Lee, Hye-Hyeon (Medi-Farm Industrialization Research Center, Dong-A University) ;
  • Seo, Kwon-Il (Department of Food Nutrition, Sunchon National University) ;
  • Kim, Kwang-Hyuk (Department of Microbiology, Kosin University College of Medicine) ;
  • Jeong, Yong-Kee (Medi-Farm Industrialization Research Center, Dong-A University)
  • 서민정 (동아대학교 Medi-Farm 산업화 연구사업단) ;
  • 강병원 (동아대학교 Medi-Farm 산업화 연구사업단) ;
  • 김민정 (동아대학교 Medi-Farm 산업화 연구사업단) ;
  • 이혜현 (동아대학교 Medi-Farm 산업화 연구사업단) ;
  • 서권일 (순천대학교 식품영양학과) ;
  • 김광혁 (고신대학교 의과대학 미생물학교실) ;
  • 정영기 (동아대학교 Medi-Farm 산업화 연구사업단)
  • Received : 2013.06.20
  • Accepted : 2013.12.03
  • Published : 2014.02.28
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Abstract

The effect of cordycepin purified from Cordyceps militaris on macrophage activation was investigated in peritoneal macrophages isolated from C57BL6 mice. Lipopolysaccharide-induced mouse peritoneal cells showed that cordycepin treatment increased the expression of the inflammatory cytokines interleukin (IL)-$1{\beta}$, IL-12, and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), leading to early inflammation-mediated reactions, the activation of immunological responses, and T lymphocyte activation. T lymphocytes, activated by a greater production of IL-6, resulted in antibody-generating immune reactions, suggesting that cordycepin was effective at inducing immunological responses. Consistent with the increase in the inflammation-mediating factors including nitric oxide (NO) and hydrogen peroxide ($H_2O_2$), the toxic response of macrophages was activated and effectively induced inflammation. These findings demonstrate that cordycepin is involved in reducing cell injury provoked by inflammatory reactions. Therefore, these results suggest that cordycepin treatment of mouse peritoneal cells induces inflammation-mediated immunological responses and immunostimulation.

본 연구는 동충하초(Cordyces militaris) 유래의 기능성 물질인 코디세핀의 면역활성을 검증하기 위하여 C57BL6 마우스 복강 대식세포를 이용하여 코디세핀이 대식세포의 활성화에 미치는 영향에 대하여 시험하였다. 그 결과 LPS에 의해 유도된 마우스 복강세포는 코디세핀의 작용에 의해 IL-$1{\beta}$, IL-12, TNF-${\alpha}$의 염증성 사이토카인의 생성이 증대되어 초기 염증매개 반응을 유도하여 선천면역반응의 활성화와 그리고 면역작용에 있어 후기 적응면역의 전환으로의 T 림프구의 활성화가 예상된다. 또한 IL-6의 생성증대로 활성화된 T 림프구에 의해 B 림프구의 항체생성반응을 매개하는 면역반응도 상승할 것으로 사료된다. 그리고 대식세포에 의한 염증반응에서 염증매개인자인 NO와 $H_2O_2$의 생성을 증대시킴에 따라 대식세포의 독성작용을 활성화시켜 염증반응을 효과적으로 유도할 것으로 보이며, 또한 $H_2O_2$의 후기 생성을 저해하였는데 이는 염증반응에 유도될 수 있는 세포의 손상으로부터 세포를 보호할 수 있을 것으로 사료된다. 따라서 코디세핀은 외부인자로부터 염증매개성 면역반응의 증강작용을 나타내는 것으로 사료된다.

Keywords

References

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