DOI QR코드

DOI QR Code

환삼덩굴(Humulus japonicus) 추출물의 항결핵 효과

Anti-mycobacterial Effects of the Extract of Humulus japonicus

  • 홍민선 (국제결핵연구소 면역병리부) ;
  • 손은순 (국제결핵연구소 면역병리부) ;
  • 이성중 (국제결핵연구소 면역병리부) ;
  • 이선경 (국제결핵연구소 면역병리부) ;
  • 이예진 (국제결핵연구소 면역병리부) ;
  • 송선대 (국제결핵연구소 면역병리부) ;
  • 조상래 (국제결핵연구소 면역병리부) ;
  • 크립톤 베리 (미국국립보건원 알레르기 및 감염병연구소 결핵연구부) ;
  • 엄석용 (국제결핵연구소 면역병리부)
  • Hong, Min-Sun (Division of Immunopathology and Cellular Immunology, International Tuberculosis Research Center) ;
  • Son, Eun-Soon (Division of Immunopathology and Cellular Immunology, International Tuberculosis Research Center) ;
  • Lee, Sung-Joong (Division of Immunopathology and Cellular Immunology, International Tuberculosis Research Center) ;
  • Lee, Sun-Kyoung (Division of Immunopathology and Cellular Immunology, International Tuberculosis Research Center) ;
  • Lee, Ye-Jin (Division of Immunopathology and Cellular Immunology, International Tuberculosis Research Center) ;
  • Song, Sun-Dae (Division of Immunopathology and Cellular Immunology, International Tuberculosis Research Center) ;
  • Cho, Sang-Nae (Division of Immunopathology and Cellular Immunology, International Tuberculosis Research Center) ;
  • Barry, Clifton E. III (Tuberculosis Research Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health) ;
  • Eum, Seok-Yong (Division of Immunopathology and Cellular Immunology, International Tuberculosis Research Center)
  • 투고 : 2013.09.13
  • 심사 : 2013.11.20
  • 발행 : 2014.02.28

초록

환삼덩굴의 항결핵효능을 알아보기 위하여 두 가지의 실험을 실시하였다. 먼저 환삼덩굴의 메탄올 추출물을 만들어 결핵균에 직접 작용시켰을 때 아주 우수한 결핵균살상효과를 보였다. 두번째로 탐식구에 감염된 결핵균에 대한 증식 억제효능이 있는지 알아 보기 위해 사람의 대식구에 메탄올 추출물을 작용시킨 후 결핵균을 감였시켰을 때 대조군에 비해 현저한 결핵균 증식억제 효과를 보였다. 환삼덩굴의 메탄올 추출물로부터 여러가지 용매를 통해 다섯 가지의 분획물을 얻어 같은 실험을 실시하였다. 이들 분획물 중 결핵균 직접 살상효과를 보인 것은 헥산과 에틸아세테이트층이었고, 탐식구에 감염된 결핵균에 대한 증식억제 효능을 보인 것은 부탄올과 물층이었다. 이처럼 환삼덩굴은 결핵균에 대해 직접적인 살상효과 뿐 아니라 탐식구에 감염되어 있는 결핵균의 증식을 억제하는 효과도 보여 주었는데 이와 같은 결과로 볼 때 환삼덩굴로부터 새로운 결핵치료물질 개발을 기대해 볼 수 있을 것으로 기대된다.

The present study aimed to evaluate the in vitro antimycobacterial effects of hop plant, Humulus japonicus. Methanol extract of H. japonicus (MeOH extract) showed strong direct bactericidal effects against Mycobacterium tuberculosis in vitro. Furthermore, the MeOH extract significantly inhibited M. tuberculosis growth in human macrophages. When five fractions obtained from MeOH extract were examined using the same methods, the hexane and ethyl acetate fractions showed bactericidal effects against M. tuberculosis in vitro, whereas the butanol and water fractions inhibited M. tuberculosis growth in macrophages. Because H. japonicus extract exhibited antimycobacterial activity against both free M. tuberculosis in culture medium and intracellular M. tuberculosis in human macrophages, this plant might be a good candidate for development of a new anti-tuberculosis drug.

키워드

참고문헌

  1. Lee DH. Annual Report on the Notified Tuberculosis Patients in Korea. Korea Centers for Disease Control & Prevention, Cheongwon, Korea. pp. 10-43 (2013)
  2. Iseman MD. Treatment of multidrug-resistant tuberculosis. New Engl. J. Med. 329: 784-791 (1993) https://doi.org/10.1056/NEJM199309093291108
  3. Park SW, Woo CJ, Chung SK, Chung KT. Antimicrobial and antioxidative activities of solvent fraction from Humulus japonicas. Korean J. Food Sci. Technol. 26: 464-470 (1994)
  4. Park SW, Kim SH, Chung SK. Antimutagenic effects and isolation of flavonoids from Humulus japonicus extract. Korean J. Food Sci. Technol. 27: 897-901 (1995)
  5. Yu BC, Yang MC, Lee KH, Kim KH, Choi SU, Lee KR. Two new phenolic constituents of Humulus japonicus and their cytotoxicity test in vitro. Arch. Pharm. Res. 30: 1471-1475 (2007) https://doi.org/10.1007/BF02977373
  6. Park JW, Ko SH, Kim CW, Jeoung BJ, Hong CS. Identification and characterization of the major allergen of the Humulus japonicus pollen. Clin. Exp. Allergy 29: 1080-1086 (1999) https://doi.org/10.1046/j.1365-2222.1999.00615.x
  7. Guenin-Mace L, Simeone R, Demangel C. Lipids of pathogenic Mycobacteria: contribution to virulence and host immune suppression. Transbound Emerg. Dis. 56: 255-268 (2009) https://doi.org/10.1111/j.1865-1682.2009.01072.x
  8. Hur JY, Jeong CH, Shim KH. Chemical components of Humulus japonicus leaves and stalks. J. Agric. Life Sci. 37: 1-7 (2003)
  9. Yu BC, Yang MC, Lee KH, Kim KH, Lee KR. Norsesquiterpene and steroid constituents of Humulus japonicus. Nat. Prod. Sci. 13: 332-336 (2007)

피인용 문헌

  1. Humulus japonicus inhibits the progression of Alzheimer's disease in a APP/PS1 transgenic mouse model vol.39, pp.1, 2017, https://doi.org/10.3892/ijmm.2016.2804
  2. Natural Dyeing of Silk Fabrics with Humulus japonicus Extract vol.26, pp.3, 2014, https://doi.org/10.5764/TCF.2014.26.3.263
  3. Antimicrobial effects of natural extracts on bacteria isolated from the uterus of Hanwoo (Korean native cattle) vol.54, pp.3, 2014, https://doi.org/10.14405/kjvr.2014.54.3.159
  4. Anti-atherogenic effect of Humulus japonicus in apolipoprotein E-deficient mice vol.38, pp.4, 2016, https://doi.org/10.3892/ijmm.2016.2727
  5. Attenuates Hyperlipidemia and Fatty Liver in Obese Mice vol.21, pp.10, 2018, https://doi.org/10.1089/jmf.2017.4135