DOI QR코드

DOI QR Code

An Investigation of the Hazards Associated with Cucumber and Hot Pepper Cultivation Areas to Establish a Good Agricultural Practices (GAP) Model

오이와 고추생산 환경에서의 GAP 모델 개발을 위한 위해요소 조사

  • Shim, Won-Bo (School of Physics and Chemistry, Gwangju Institute of Science and Technology) ;
  • Lee, Chae-Won (Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Jeong, Myeong-Jin (Department of Food and Drug, Graduate of Food and Drug, Gyeongsang National University) ;
  • Kim, Jeong-Sook (Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Ryu, Jae-Gee (Microbial Safety Division, Department of Agro-Food Safety, NAAS, RDA) ;
  • Chung, Duck-Hwa (Institute of Agriculture and Life Science, Gyeongsang National University)
  • 심원보 (광주과학기술원 물리화학부) ;
  • 이채원 (경상대학교 농업생명과학연구원) ;
  • 정명진 (경상대학교 식의약품학과) ;
  • 김정숙 (경상대학교 농업생명과학연구원) ;
  • 류재기 (농촌진흥청 국립농업과학원 농산물안전성부 유해생물팀) ;
  • 정덕화 (경상대학교 농업생명과학연구원)
  • Received : 2013.09.30
  • Accepted : 2013.10.31
  • Published : 2014.02.28

Abstract

To analyze the hazards associated with cucumber and hot pepper cultivation areas, a total of 72 samples were obtained and tested to detect the presence of biological (sanitary indicative, pathogenic bacteria and fungi) and chemical hazards (heavy metals and pesticide residues). The levels of sanitary indicative bacteria (aerobic plate counts and coliforms) and fungi were ND-7.2 and ND-4.8 log CFU/(g, mL, hand, or $100cm^2$) in cucumber cultivation areas, and ND-6.8 and 0.4-5.3 log CFU/(g, mL, hand, or $100cm^2$) in hot pepper cultivation areas. More specifically, the soil of hot pepper cultivation areas was contaminated with coliforms at a maximum level of 5.6 log CFU/g. Staphylococcus aureus was detected only in glove samples at a level of 1.4 log CFU/$100cm^2$ and Bacillus cereus was detected in the majority of samples at a level of ND-4.8 log CFU/(g, mL, hand, or $100cm^2$). Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella spp. were not detected. Heavy metal (Zn, Cu, Ni, Pb, and Hg) chemical hazards were detected at levels lower than the regulation limit. Residual insecticides were not detected in cucumbers; however, hexaconazole was detected at a level of 0.016 mg/kg (maximum residue limit: 0.3 mg/kg) in hot peppers.

오이와 고추의 안전성에 영향을 미치는 위해요소 분석의 기초자료로 활용하기 위해 오이와 고추 재배지에서 총 72점의 시료를 채취하여 미생물학적(위생지표세균, 병원성미생물, 곰팡이) 위해요소와 화학적(중금속, 잔류농약) 위해요소를 조사하였다. 미생물학적 위해요소 중 위생지표세균과 곰팡이는 오이 재배지에서 ND-7.2와 ND-4.8 log CFU/g (g, mL, hand, or $100cm^2$) 범위로 검출되었고, 고추 재배지에서는 ND-6.8와 0.4-5.3 log CFU/g (g, mL, hand, or $100cm^2$) 범위로 검출되었다. 특히 대장균군은 고추재배지의 토양에서 최대 5.6 log CFU/g까지 검출되었다. 병원성 미생물은 S. aureus는 오이 농장의 장갑에서만 1.4 log CFU/$100cm^2$으로 검출되었고, B. cereus는 대부분의 시료에서 ND-4.8 log CFU/g (g, mL, hand, or $100cm^2$) 범위로 검출되었다. L. monocytogenes, E. coli O157:H7 및 Salmonella spp.는 검출되지 않았다. 화학적 위해요소에 해당하는 중금속은 Zn, Cu, Ni, Pb 및 Hg이 국내 허용기준치 이하로 검출되었고, 잔류농약의 경우 오이에서는 모든 성분이 불검출 되었으나 고추에서 hexaconazole 성분이 0.016 mg/kg (기준치: 0.3 mg/kg)으로 검출되었다.

Keywords

References

  1. Yun HJ, Park KH, Ryu KY, Kim BS. Analyses of microbiological contamination in cultivation and distrubution stage of tomato and evaluation of microbial growth in tomato extract. J. Fd. Hyg. Safety 28: 174-180 (2013) https://doi.org/10.13103/JFHS.2013.28.2.174
  2. Lee WJ, Lee CH, Yoo JY, Kim KY, Jang KI. Sterilization efficacy of washing method using based on microbubbles and electrolyzed water on various vegetables. J. Korean Soc. Food Sci. Nutr. 40: 912-917 (2011) https://doi.org/10.3746/jkfn.2011.40.6.912
  3. Hong CK, Seo YH, Choi CM, Hwang IS, Kim MS. Microbial quality of fresh vegetables and fruits in seoul, Korea. J. Fd. Hyg. Safety 27: 24-29 (2012) https://doi.org/10.13103/JFHS.2012.27.1.024
  4. Jin YG, Kim TW, Ding T, Oh DH. Effect of electrolyzed water and citric acid on quality enhancement and microbial inhibition in Head Lettuce. Korean J. Food Sci. Technol. 41: 578-586 (2009)
  5. Kim SR, Ryu KY, Lee MH, Jung CS, Yoon YH, Shim WB, Kim JH, Kim BS, Yoo SY, Kim DH, Yun JC, Chung DH. Evaluation of the bactericidal activity of electrolyzed water against Salmonella Typhimurium and Staphylococcus aureus on perilla leaves. Korean J. Food Sci. Technol. 44: 467-471 (2012) https://doi.org/10.9721/KJFST.2012.44.4.467
  6. Kim C, Hung YC, Brachett RE. Efficacy of electrolyzed oxidizing (EO) and chemically modified water on different types of food-borne pathogens. Int. J. Food Microbiol. 61: 199-207 (2000) https://doi.org/10.1016/S0168-1605(00)00405-0
  7. National Agricultural Products Quality Management Service. Good Agricultural Practice (GAP). Available from: https://www.gap.go.kr. Accessed Sep. 5, 2013.
  8. Huh EJ, Kim JW. Consumer knowledge and attitude to spending on environment-friendly agricultural products. Korean J. Hum. Ecol. 19: 883-896 (2010) https://doi.org/10.5934/KJHE.2010.19.5.883
  9. Anonymous. Guidelines for effectiveness testing of surgical hand scrub(glove juice test). Fed. Regist. 43: 1242-1243 (1978)
  10. MFDS. Korean Foods Code. Ministry of Food and Drug Safety, Cheongwon, Korea (2013)
  11. RDA. Textbook for training GAP inspector. Rural Development Administration. Suwon, Korea. pp. 59-64 (2012)
  12. Lehotay SJ. Determination of pesticide residues in foods by acetonitrile extraction and partitioning with magnesium sulfate: collaborative study. J. AOAC Int. 90: 485-520 (2007)
  13. Nam Mj, Heo RW, Lee WG, Kim KY, Chung DY, Kim JS, Shim WB, Chung DH. Microbiological hazard analysis of hot pepper farms for the application of good agricultural practices (GAP) system. J. Agri. Life Sci. 45: 163-173 (2011)
  14. Kim BY, Weon HY, Park IC, Lee SY, Kim WG, Song JK. Microbial diversity and community analysis in lettuce or cucumber cultivated greenhouse soil in Korea. Korean J. Soil Sci. Fert. 44: 1169-1175 (2011) https://doi.org/10.7745/KJSSF.2011.44.6.1169
  15. Joa JH, Moon DG, Koh SW, Hyun HN. Effect of temperature condition on nitrogen mineralization and soil microbial community shift in volcanic ash soil. Korean J. Soil Sci. Fert. 45: 467-474 (2012) https://doi.org/10.7745/KJSSF.2012.45.4.467
  16. Solomon EB, Yaron S, Mattews KR. Transmission of Escherichia coli O157:H7 from contaminated manure and irrigation water to lettuce plant tissue and its subsequent internalization. Appl. Environ. Microbiol. 68: 397-400 (2002) https://doi.org/10.1128/AEM.68.1.397-400.2002
  17. An YJ, Peter BG. Monitoring E. coli and total coliforms in natural spring water as related to recreational mountain areas. Environ. Monit. Assess. 102: 131-137 (2005) https://doi.org/10.1007/s10661-005-4691-9
  18. Jung KS, Roh EJ, Ryu KY, Kim WI, Park KH, Lee DH, Kim KH, Yun JC, Heu SG. Monitoring of pathogenic bacteria in organic vegetables from Korean market. Korean J. Soil Sci. Fert. 45: 560-564 (2012) https://doi.org/10.7745/KJSSF.2012.45.4.560
  19. Bae YM, Hong YJ, Kang DH, Heu SG, Lee SY. Microbial and pathogenic contamination of ready-to-eat fresh vegetables in Korea. Korean J. Food Sci. Technol. 43: 161-168 (2011) https://doi.org/10.9721/KJFST.2011.43.2.161
  20. Choi JW, Park SY, Yeon JH, Lee MJ, Chung DH, Lee KH, Kim MG, Lee DH, Kim KS, Ha SD. Microbial contamination levels of fresh vegetables distributed in markets. J. Fd. Hyg. Safety. 20: 43-47 (2005)
  21. Jo SH, Chung HJ, Lee SH, Hwang SJ, Om AS, Eun JB. Microbial quality of fresh vegetables in restaurants around school. Korean J. Food Preserv. 20: 424-428 (2013) https://doi.org/10.11002/kjfp.2013.20.3.424
  22. Kim SR, Lee JY, Lee SH, Ko HS, Yoon YH, Kwon SH, Ryu KY, Yun HJ , Kim WI, Yun JC, Kim DH, Chung DH. Distribution of microorganisms in perilla leaf and cultivation area. Korean J. Food Sci. Technol. 43: 243-248 (2011) https://doi.org/10.9721/KJFST.2011.43.2.243
  23. Yu YM, Oh SC, Sung BJ, Kim HH, Lee YH, Youn YN. Analysis of good agricultural practices (GAP) in panax ginseng C.A. mayer. Korean J. Medicinal Crop Sci. 15: 220-226 (2007)
  24. Kim KY, Song JE, Heo RW, Lee WG, Nam MJ, Kim JS, Shim WB, Gil JG, Jung CS, Park KY, Chung DH. Investigation and analysis of hazards for cultivation environment to establish the good agricultural practices (GAP) of soybean. J. Agri. Life Sci. 44: 121-132 (2010)
  25. Kim MR, Na MA, Jung WY, Kim CS, Sun NK, Seo EC, Lee EM, Park YG, Byun JA, Eom JH, Jung RS, Lee JH. Monitoring of pesticide residues in special products. Korean J. Pestic. Sci. 12: 323-334 (2008)
  26. Han SH, Park SK, Kim OH, Choi YH, Seoung HJ, Lee YJ, Jung JH, Kim YH, Yu IS, Kim YK, Han KY, Chae YZ, Monitoring of pesticide residues in commercial agricultural products in the northern area of Seoul, Korea. Korean J. Pestic. Sci. 16: 109-120 (2012) https://doi.org/10.7585/kjps.2012.16.2.109
  27. Nam HS, Choi YH, Yoon SH, Hong HM, Park YC, Lee JH, Kang YS, Lee JO, Ahn YS, Hong YP, Kim HY. Monitoring of residual pesticide in commercial agricultural products. Korean J. Food Sci. Technol. 38: 317-324 (2006)

Cited by

  1. Investigation of Microbial Contamination in Oenanthe javanica at Postharvest Environments vol.29, pp.4, 2014, https://doi.org/10.13103/JFHS.2014.29.4.268
  2. Evaluation on Microbial Contamination in Red Pepper and Red Pepper Cultivated Soil in Korea vol.33, pp.5, 2018, https://doi.org/10.13103/JFHS.2018.33.5.347