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Gene Flow from GM Cabbage to Non-GM Control

유전자변형 양배추로부터 비유전자변형 모본으로의 유전자 이동성

  • Kim, Young-Joong (Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Nam, Kyong Hee (Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Pack, In Soon (Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Jung-Ho (Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jeong, Soon-Chun (Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Harn, Chee Hark (R&D Headquarter, Nongwoo Bio Co.) ;
  • Kim, Chang-Gi (Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology)
  • 김영중 (한국생명공학연구원 바이오평가센터) ;
  • 남경희 (한국생명공학연구원 바이오평가센터) ;
  • 백인순 (한국생명공학연구원 바이오평가센터) ;
  • 박정호 (한국생명공학연구원 바이오평가센터) ;
  • 정순천 (한국생명공학연구원 바이오평가센터) ;
  • 한지학 (농우바이오 R&D 본부) ;
  • 김창기 (한국생명공학연구원 바이오평가센터)
  • Received : 2014.09.01
  • Accepted : 2014.09.17
  • Published : 2014.09.30

Abstract

Understanding the gene flow from genetically modified (GM) crops to conventional crops is important to prevent and mitigate seed contamination caused by pollen-mediated gene flow. We conducted a field test to investigate the gene flow from diamondback moth resistant GM cabbage (Brassica oleracea var. capitata) containing cry1Ac1 gene, to a non-GM control line AD126. GM and non-GM cabbage plants were cultivated in the field and pollinated using Bombus terrestris under the nets during the flowering periods. After seeds were collected from non-GM plants, hybrids between them and the GM cabbages were screened by multiplex PCR targeting cry1Ac1 gene. Out of 878 germinated seedlings, 168 hybrids were found and the average gene flow frequency was 19.7%. Because cabbage is mainly pollinated by insect pollinators, large-scale field tests are needed to study gene flow of GM cabbage.

Keywords

References

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