Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Production of DNA polymerase from Thermus aquaticus in recombinant Escherichia coli

  • Kim, Sung-Gun (Department of Biomedical Science, Youngdong University) ;
  • Park, Jong-Tae (Department of Food Science and Technology, Chungnam National University)
  • Received : 2014.05.30
  • Accepted : 2014.08.14
  • Published : 2014.09.30
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Abstract

Among dozens of DNA polymerases cloned from thermophilic bacteria, Taq DNA polymerase from Thermus aquaticus has been most frequently used in polymerase chain reaction (PCR) that is being applied to gene cloning, DNA sequencing, gene expression analysis, and detection of infectious and genetic diseases. Since native Taq DNA polymerase is expressed at low level in T. aquaticus, recombinant Escherichia coli system was used to produce Taq DNA polymerase in a large amount. Taq DNA polymerase was expressed as a soluble form under the control of tac promoter in E. coli, and purified by heat treatment and ion exchange chromatographies. The purified Taq DNA polymerase was nearly homogeneous and exhibited a similar DNA amplification activity with a commercial Taq DNA polymerase.

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References

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