Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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The Effects of a Dietary Edwardsiella tarda Specific Bacteriophage and Bacillus subtilis Mixture on Innate Immune Responses and Antibacterial Activity of Nile tilapia Oreochromis niloticus

Edwardsiella tarda의 특이 Bacteriophage와 Bacillus subtilis가 혼합된 사료급이가 나일 틸라피아(Oreochromis niloticus)의 선천적 면역반응과 항균효과에 미치는 영향

  • Baek, Min Suk (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Hwang, Yo Sep (Department of Aquatic Life Medicine, Kunsan National University) ;
  • Choi, Sanghoon (Department of Aquatic Life Medicine, Kunsan National University)
  • 백민석 (군산대학교 수산생명의학과) ;
  • 황요셉 (군산대학교 수산생명의학과) ;
  • 최상훈 (군산대학교 수산생명의학과)
  • Received : 2013.10.04
  • Accepted : 2014.02.04
  • Published : 2014.02.28
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Abstract

The present study investigated the effects of dietary Edwardsiella tarda (E. tarda) specific bacteriophage (phage) and Bacillus subtilis (B. subtilis) mixture on innate immune responses and antibacterial activity of Nile tilapia, Oreochromis niloticus. In a dietary experiment, tilapia were fed the control diet (C), a phage-only supplemented diet (P), a B. subtilis only supplemented diet (B), or a B. subtilis and phage mixed diet (B+P). A respiratory burst and significant increase in lysozyme activity (P<0.05) were noted in the B+P group, as compared to other groups after 4 days of feeding. The B group showed a significant (P<0.05) increase in respiratory burst and lysozyme activity versus the C and P groups, whereas no significant increases (P<0.05) were observed in the P and C groups. $ACH_{50}$ was significantly up-regulated in the B+P group versus other groups after 8 days of feeding (P<0.05). In vivo antibacterial activity was significantly enhanced in the B+P fed group, as compared to other groups (P<0.05) after 7 days of E. tarda challenge. A significant (P<0.05) increase in antibacterial activity was seen in the B group, as compared to C or P groups after 14 days of feeding. These results suggest that a B. subtilis and phage mixture could be utilized as an alternative to antibiotics in the control of fish diseases caused by E. tarda.

Keywords

References

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