Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Cryopreservation of Common Korean Bitterling Acheilognathus signifer Sperm

묵납자루(Acheilognathus signifer) 정자의 냉동보존

  • Jeong, Min Hwan (Aquaculture Management Division, National Fisheries Research and Development Institute) ;
  • Min, Byung Hwa (Aquaculture Management Division, National Fisheries Research and Development Institute) ;
  • Park, Mi Seon (Aquaculture Management Division, National Fisheries Research and Development Institute) ;
  • Myeong, Jeong-In (Aquaculture Management Division, National Fisheries Research and Development Institute) ;
  • Im, Je Hyun (Inland Aquaculture Research Center, National Fisheries Research & Development Institute) ;
  • Lim, Han Kyu (Development of Marine and Fisheries Resources, Mokpo National University) ;
  • Hwang, Hyung-Kyu (Aquaculture Management Division, National Fisheries Research and Development Institute)
  • 정민환 (국립수산과학원 양식관리과) ;
  • 민병화 (국립수산과학원 양식관리과) ;
  • 박미선 (국립수산과학원 양식관리과) ;
  • 명정인 (국립수산과학원 양식관리과) ;
  • 임재현 (국립수산과학원 내수면양식연구센터) ;
  • 임한규 (목포대학교 해양수산자원학과) ;
  • 황형규 (국립수산과학원 양식관리과)
  • Received : 2013.10.25
  • Accepted : 2014.01.07
  • Published : 2014.02.28
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Abstract

This study aimed to find an optimal diluent and cryoprotective agent (CPA) during cryopreservation of common Korean bitterling Acheilognathus signifier sperm. The bitterling is an endangered species in Korea, and this study will enable conservation and further technical development of artificial seed production. We tested the effects of cryopreservation and toxicity by the type of diluent and CPA. The optimal combination of diluent and CPA for cryopreservation was 300 mM glucose+10% methanol, resulting in a survival rate and sperm activity index (SAI) of $96.3{\pm}1.5%$, and $3.0{\pm}0.0$ respectively, and no significant difference compared to fresh sperm. The survival rate and SAI of post-thawed sperm was $9.7{\pm}1.5%$, $0.8{\pm}0.3$ respectively, which was significantly higher than had been achieved with other diluents and CPAs.

Keywords

References

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