Polymer(Korea) (폴리머)

The Polymer Society of Korea (한국고분자학회)
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Inflammatory Responses to Hydroxyapatite/Poly(lactic-co-glycolic acid) Scaffolds with Variation of Compositions

하이드록시아파타이트/락타이드 글리콜라이드 공중합체 지지체 조성에 따른 염증 완화 효과

  • Jang, Ji Eun (Dept. of BIN Fusion Tech., and Dept. of Polymer-Nano Sci. & Tech. and Polymer BIN Research Center, Chonbuk National University) ;
  • Kim, Hye Min (Dept. of BIN Fusion Tech., and Dept. of Polymer-Nano Sci. & Tech. and Polymer BIN Research Center, Chonbuk National University) ;
  • Kim, Hyeongseok (Dept. of BIN Fusion Tech., and Dept. of Polymer-Nano Sci. & Tech. and Polymer BIN Research Center, Chonbuk National University) ;
  • Jeon, Dae Yeon (Dept. of BIN Fusion Tech., and Dept. of Polymer-Nano Sci. & Tech. and Polymer BIN Research Center, Chonbuk National University) ;
  • Park, Chan Hum (Dept. of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, College of Medicine, Hallym University) ;
  • Kwon, Soon Yong (Dept. of Orthopedic Surgery, Yeouido St. Mary's Hospital, Catholic University of Korea) ;
  • Chung, Jin Wha (Dept. of Orthopedic Surgery, Yeouido St. Mary's Hospital, Catholic University of Korea) ;
  • Khang, Gilson (Dept. of BIN Fusion Tech., and Dept. of Polymer-Nano Sci. & Tech. and Polymer BIN Research Center, Chonbuk National University)
  • 장지은 (전북대학교 BIN 융합공학과, 고분자 나노공학과) ;
  • 김혜민 (전북대학교 BIN 융합공학과, 고분자 나노공학과) ;
  • 김형석 (전북대학교 BIN 융합공학과, 고분자 나노공학과) ;
  • 전대연 (전북대학교 BIN 융합공학과, 고분자 나노공학과) ;
  • 박찬흠 (한림대학교 의과대학 춘천성심병원 이비인후과) ;
  • 권순용 (가톨릭대학교 의과대학 여의도성모병원 정형외과) ;
  • 정진화 (가톨릭대학교 의과대학 여의도성모병원 정형외과) ;
  • 강길선 (전북대학교 BIN 융합공학과, 고분자 나노공학과)
  • Received : 2013.09.17
  • Accepted : 2013.11.06
  • Published : 2014.03.25
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Abstract

Hydroxyapatite has osteoconductivity, biocompatibility and noninflammatory, and it has been used clinically as artificial bone. In this study, we prepared hydroxyapatite/poly(lactic-co-glycolic acid) (PLGA) scaffolds using 0, 10, 20, 40 and 60 wt% of hydroxyapatite. We analyzed compressive strength, SEM analysis and FTIR for mechanical property of 3D hydroxyapatite/PLGA scaffolds. For biocompatibility tests, cell proliferation and viability were measured via MTT assay and SEM. We analyzed RT-PCR, FACS, histology (H&E, ED-1) for anti-inflammatory effect. This study showed that hydroxyapatite hybrid scaffolds have low inflammatory reaction compared with the PLGA. This result has a potential for the application of artificial bone graft material.

하이드록시아파타이트는 골 전도가 우수하고 생체 적합성이 우수하며 염증 반응을 일으키지 않아 임상에서 골이식재로 널리 사용되고 있다. 본 연구에서는 하이드록시아파타이트를 함유한 poly(lactic-co-glycolic acid) (PLGA) 지지체를 제조하였으며 생체 내/외의 실험을 통하여 골 이식재로서의 응용가능성을 평가하였다. 하이드록시아파타이트/PLGA 지지체는 0, 10, 20, 40 및 60 wt%의 함량으로 제조하였다. 기계적 특성을 알아보기 위하여 압축강도, SEM, FTIR을 측정하였으며 MTT, RT-PCR, FACS, 조직학적 염색(H&E, ED-1)을 실시하였다. 그 결과 하이드록시아파타이트를 함유한 PLGA 지지체에서 염증 반응이 감소하는 것을 확인할 수 있었으며 골 이식재로서의 가능성을 보여주었다.

Keywords

Acknowledgement

Supported by : 농림수산식품부, 한국연구재단

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