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Antioxidative Activity of Feral Haw (Crataegus pinnatifida BUNGE) Seed Extracts Using Various Solvents

자생 산사(Crataegus pinnatifida BUNGE)씨의 추출 용매에 따른 항산화 활성

  • Kim, Min-A (Department of Food Science & Technology, Pusan National University) ;
  • Duan, Yishan (Department of Food Science & Technology, Pusan National University) ;
  • Seong, Jong-Hwan (Department of Food Science & Technology, Pusan National University) ;
  • Chung, Hun-Sik (Department of Food Science & Technology, Pusan National University) ;
  • Kim, Han-Soo (Department of Food Science & Technology, Pusan National University)
  • Received : 2014.01.22
  • Accepted : 2014.02.06
  • Published : 2014.02.28

Abstract

The purpose of this study was to examine the antioxidative activity of feral haw (Crataegus pinnatifida BUNGE) seed extracts using 70% methanol, 70% ethanol, chloroform:methanol (CM, 2:1, v/v), n-butanol and ethyl acetate (EA). The total phenol content of the five extracts ranged from 37.29 mg/g to 55.53 mg/g. Moreover, the content was high in the 70% methanol and, 70% ethanol extracts, but low in the n-butanol extract. On the contrary, the total flavonoid content decreased in the order of n-butanol (2.93 mg/g), EA (2.67 mg/g), 70% methanol (1.00 mg/g), 70% ethanol (0.88 mg/g) and CM (0.67 mg/g) extracts. The $NO_2$ radical scavenging activity, antioxidant activity by ${\beta}$-carotene bleaching assay and, superoxide dismutase (SOD) like ability decreased in the order of 70% methanol, 70% ethanol, CM, EA and, n-butanol extracts; further, a similar tendency was also observed in the total phenol contents. Overall, these results indicated that the antioxidative activity of feral haw seeds was closely related to the total phenol and flavonoid contents. Therefore, haw seeds might be usefully applied to natural antioxidants as well as functional foods.

Keywords

References

  1. Aughsteen AA, Mohammed FI. 2002. Insulin enhances amylase and lipase activityin the pancreas of streptozotocin-diabetic rats. Saudi Med J 23: 838 -844
  2. Bae MH, Kim HH. 2003. Mechanism of Crataegi fructus extract induced endothelium-dependent vasorelaxation in rabbit carotid artery. Korean J Herb 18: 169-180
  3. Choi HS, Beik KY, Kim JB. 2012. Studies on antioxidative effects of Schisandra chinensis seed extract. J Korean Soc Cosmet 18: 908-915
  4. Choi YM, Kim MH, Shin JJ, Park JM, Lee JS. 2003. The antioxidant activities of the some commercial teas. J Korean Soc Food Sci Nutr 32: 723-727 https://doi.org/10.3746/jkfn.2003.32.5.723
  5. Chon JW, Park JK, Lee MA, Jeong MR, Han JH, Park YK. 2009. Fermented Crataegi fructus vinegar improves lipid metabolism in rats fed high fat diet. J Korean Soc Food Sci Nutr 38: 1024-1031 https://doi.org/10.3746/jkfn.2009.38.8.1024
  6. Chon JW, Park SJ, Han JH, Park SH. 2005. Study of Crataegi fructus for medicinal foods applications - nutrition composition and scheme for foods -. Korean J Oriental Phys Path 19: 1220-1224
  7. Dillard CJ, German JB. 2000. Phytochemicals: nutraceuticals and human health. J Sci Food Agric 80: 1744-1756 https://doi.org/10.1002/1097-0010(20000915)80:12<1744::AID-JSFA725>3.0.CO;2-W
  8. Goulas V, Manganaris GA. 2012. Exploring the phytochemical content and the antioxidant potential of Citrus fruits grown in cyprus. Food Chem 131: 39-47. https://doi.org/10.1016/j.foodchem.2011.08.007
  9. Gutfinger T. 1981. Polyphenols in olive oils. J Am Oil Chem Soc 966-968
  10. Han JS, Rhee SH. 1996. The relationship between serum cholesterol level and dieatry intake in obese children, J Korean Soc Food Nutr 25: 433-440
  11. Heim KE, Tagliaferro AR, Bobilya DJ. 2002. Flavonoid antioxidants:chemistry, metabolism and structure - activity relationships. J Nutr Biochem 13: 572- 584 https://doi.org/10.1016/S0955-2863(02)00208-5
  12. Hwang IW, Lee HR, Kim SK, Zheng HZ, Choi JU, Lee SH, Lee SH, Chung S K. 2008. Proanthocyanidin content and antioxidant characteristics of grape seeds. Korean J Food Preserv 15: 859-863
  13. Jang EH, Pyo YH, Ahn MS. 1996. Antioxidant effect of omija (Schizandra chinesis Baillon) extracts. Korean J Soc Food Sci 12: 372-376
  14. Jang JK, Han JY. 2002. The antioxidant ability of grape seed extracts. Korean J Food Sci Technol 34: 524-528
  15. Jayaprakasha GK, Singh RP, Sakariah KK. 2001. Antioxidant activity of grape seed (Vitis vinifera) extracts on peroxidation models in vitro. Food Chem 73: 285-290 https://doi.org/10.1016/S0308-8146(00)00298-3
  16. Jeong CH, Choi SG, Heo HJ. 2008. Analysis of nutritional compositions and antioxidative activities of Korean commercial blueberry and raspberry. J Korean Soc Food Sci Nutr 37: 1375-1381 https://doi.org/10.3746/jkfn.2008.37.11.1375
  17. Jeong CH, Nam EK, Shim KH. 2006. Antioxidative activities and nitrate scavenging activity in different parts of Erigeron annuus. J Agric Life Sci 40: 13-20
  18. Jeong KY, Kim ML. 2012. Physiological activities of Ulmus pumila L. extracts. Korean J Food Preserv 19: 104-109 https://doi.org/10.11002/kjfp.2012.19.1.104
  19. Ju JC, Shin JH, Lee SJ, Cho HS, Sung NJ. 2006. Antioxidative activity of hot water extracts from medicinal plants. J Korean Soc Food Sci Nutr 35: 7-14 https://doi.org/10.3746/jkfn.2006.35.1.007
  20. Jung GT, Ju IO, Choi JS, Hong JS. 2000. The antioxidative, antimicrobial and nitrite scavenging effects of Schizandra chinensis RUPRECHT (omija) seed. Korean J Food Sci Technol 32: 928-935
  21. Kang JY, Kim SY, Lee MS, Ahn HS. 2005. Effects of vegetables juice supplementation on serum lipid profile and antioxidant activity in college women. Korean J Comm Nutr 10: 183-188
  22. Kang YH, Park YK, Lee GD. 1996. The nitrite scavenging and electron donating ability of phenolic compounds. Korean J Food Sci Technol 28: 232-239
  23. Kato H, Lee IE, Chuyen NV, Kim SB, Hayase F. 1987. Inhibition of nitrosamine formation by nondialyzable melanoidins. J Agric Biol Chem 51: 1333-1338 https://doi.org/10.1271/bbb1961.51.1333
  24. Kim JH, Suh JK, Kang YH. 2012. Anticancer effects of the extracts of oriental melon (Cucumis melo L. var makuwa Makino) seeds. Korean J Plant Res 25: 647-651 https://doi.org/10.7732/kjpr.2012.25.5.647
  25. Kim JS, Choi SY. 2008. Physicochemical properties and antioxidative activities of omija (Schizandra chinensis Bailon). Korean J Food Nutr 21: 35-42
  26. Kim NY, Choi JH, Kim YG, Jang MY, Moon JH, Park GH, Oh DH. 2006. Isolation and identification of an antioxidant substance from ethanol extract of wild grape (Vitis coignetiea) seed. Korean J Food Sci Technol 38: 109-113
  27. Kim OH, Park JK. 2012. The effects of participation in exercise and nutrition education program on physical fitness, dietary habits and nutrition intake status for adolescents. Korean J Obesity 21: 158-165 https://doi.org/10.7570/kjo.2012.21.3.158
  28. Kim JS, Lee GD, Kwon JH, Yoon HS. 1993. Iden -tification of phenolic antioxidative components in Crataegus pinnatifida Bunge. J Korean Agric Chem Soc 36, 154-157.
  29. Kubola J, Siriamornpun S. 2008. Phenolic contents and antioxidant activities of bitter gourd (Momordica charantia L.) leaf, stem and fruit fraction extracts in vitro. Food Chem 110: 881-890 https://doi.org/10.1016/j.foodchem.2008.02.076
  30. Kwok CY, Wong CNY, Yau MYC, Yu PHF, Au ALS, Poon CCW, Seto SW, Lam TY, Kwan YW, Chan SW. 2010. Consumption of dried fruit of Crataegus pinnatifida (hawthorn) suppresses high-cholesterol diet-induced hypercholesterolemia in rats. J Functional Foods 2: 179-186 https://doi.org/10.1016/j.jff.2010.04.006
  31. Kwon HJ, Park CS. 2008. Biological activities of extracts from omija (Schizandra chinensis Baillon). Korean J Food Preserv 15: 587-592
  32. Kwon SH, Kim JB. 2010. Effects of Crataegii Fructus on the diet-induced hyperlipidemia in rats. Korean J Oriental Phys Path 24: 67-73
  33. Lee EJ, Bae JH. 2011. Study on the alleviation of an alcohol induced hangover and the antioxidant activity by mulberry fruit. Korean J Food Nutr 24: 204-209 https://doi.org/10.9799/ksfan.2011.24.2.204
  34. Lee GY, Jang DS, Lee YM, Kim YS, Lim JS. 2008. Constituents of the seeds of Cornus officinalis with inhibitory activity on the formation of advanced glycation end products (AGEs). J Korean Soc Appl Biol Chem 51: 316-320 https://doi.org/10.3839/jksabc.2008.055
  35. Lee HJ, Choi MS. 1999. Measurement of inhibitory activities on 3-hydroxy-3- methylglutaryl CoA reductase and acyl-CoA: cholesterol acyltransferase by various plant extracts in vitro. J Korean Soc Food Sci Nutr 28: 958-962
  36. Lee SC, Kim SY, Heong SM, Park JH. 2006. Effect of far-infrared irradiation on catechins and nitrite scavenging activity of green tea. J Agric Food Chem 54: 399-403 https://doi.org/10.1021/jf051866x
  37. Lee SH, Jeong EJ, Jung TS, Park LY. 2009. Antioxidant activities of seasoning sauces prepared with Geranium thunbergii sieb. et Zucc. and Crataegi fructus and the quality changes of seasoned pork during storage. Korean J Food Sci Technol 41: 57-63
  38. Lee SH, Kang KM, Park HJ, Baek LM. 2009. Physiological characteristics of medicinal plant extracts for use as functional materials in seasoning sauce for pork meat. Korean J Food Sci Technol 41: 100-105
  39. Lim JD, Yu CY, Kim MJ, Yun SJ, Lee SJ, Kim NY, Chung IM. 2004. Comparision of SOD activity and phenolic compound contents in various Korean medicinal plants. Korean J Medicinal Crop Sci 12: 191-202
  40. Liu P, Yang B, Kallio H. 2010. Characterization of phenolic compounds in Chinese hawthorn (Crataegus pinnatifida Bge. var. major) fruit by high performance liquid chromatography - lectrospray ionization mass spectrometry. Food Chem 121: 1188-1197 https://doi.org/10.1016/j.foodchem.2010.02.002
  41. Liu RH. 2003. Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals. Am J Clin Nutr 78: 517-520
  42. Liu T, Cao Y, Zhao M. 2010. Extraction optimization, purification and antioxidant activity of procyanidins from hawthorn (C. pinnatifida Bge. var. major) fruits. Food Chem 119: 1656-1662 https://doi.org/10.1016/j.foodchem.2009.09.001
  43. Marklund S, Marklund G. 1974. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47: 469-474 https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  44. McCord JM, Fridovich I. 1969. Superoxide dismutase an enzymic function for erythrocuprein (hemocuprein). J Biol Chem 244: 6049-6055
  45. Mello BCBS, Petrus JCC, Hubinger MD. 2010. Concentration of flavonoids and phenolic compounds in aqueous and ethanolic propolis extracts through nanofiltration. J Food Engin 96: 533-539 https://doi.org/10.1016/j.jfoodeng.2009.08.040
  46. Oh IS, Kim IH. 1993. Pharmaco-constituents of Crataegus pinnatifida var. psilosa leaves(I). Korean J Pharmacogn 37: 476-482
  47. Park CS, Yang KM, Kim ML. 2006. Functional properties of medicinal plant extracts. Korean J Food Cook Sci 23: 720-727
  48. Park JK, Kweon SH, Kim YH, Jang MJ, Oh KW. 2012. Dietary behaviors related to metabolic syndrome in Korean adults. Korean J Comm Nutr 17: 664-675 https://doi.org/10.5720/kjcn.2012.17.5.664
  49. Park SH, Kim YH, Chon JW, Song YJ, Han JH. 2005. Study of Crataegi Fructus for medicinal foods applications - functional evaluation of fermented liquid on the lipid profile improvement high fat diet -. Korean J Oriental Phys 19: 1272-1280
  50. Park SJ, Shin EH, Lee JH. 2012. Biological activities of solvent fractions from methanolic extract of Crataegi fructus. Korean J Food Nutr 25: 897-902 https://doi.org/10.9799/ksfan.2012.25.4.897
  51. Park SW, Yook CS, Lee HK. 1994. Chemical components from the fruits of Crataegus pinnatifida var. psilosa. Korean J Pharmacogn 25: 328-335
  52. Ryu HY, Ahn SM, Kim JS, Jung IC, Sohn HY. 2010. Antimicrobial activity of fruit of Crataegus pinnatifida Bunge against multidrug resistant pathogenic Pseudomonas aeruginosa and Candida sp. Korean J Microbiol Biotechnol 38: 77-83
  53. Ryu HY, Kim YK, Kwon IS, Kwon CS, Jin IN, Sohn HY. 2007. Thrombin inhibition activity of fructus extract of Crataegus pinnatifida Bunge. J Life Science 17: 535-539 https://doi.org/10.5352/JLS.2007.17.4.535
  54. Seo YJ, Kim MH, Kim MH, Choi MK. 2012. Status and relationships among lifestyle, food habits, and stress scores of adults in chungnam. Korean J Community Nutr 17: 579-588 https://doi.org/10.5720/kjcn.2012.17.5.579
  55. Shin JH, Jo MJ, Park SM, Park SJ, Kim SC. 2010. Hepatoprotective activity of Crataegii fructus water extract against cadmium-induced toxicity in rats. Korean J Oriental Phys Path 24: 249-257
  56. Son SM. 2004. Nutrition and health of sunsik consumer. Korean J Food Nutr 17: 103-109
  57. Song TH, Choi HS, Kim YS, Woo IA. 2012. Study on sensory and mechanical characteristics of white bread containing different levels of Korean and Chinese sansa (Crataegus pinnatifida Bunge) powder. Korean J Food Culture 27: 391-399 https://doi.org/10.7318/KJFC/2012.27.4.391
  58. Sung KS, Chun C, Kwon YH, Kim KH, Chang CC. 2000. Effects of red ginseng component on the antioxidative enzymes activities and lipid peroxidation in the liver of mice. J Ginseng Res 24: 29-34
  59. Tadic VM, Dobric S, Markovic GM, Dordevic SM, Arsic IA, Menkovic NR, Stevic T. 2008. Anti-inflammatory, gastroprotective, free radical scavenging and antimicrobial activities of hawthorn berries ethanol extract. J Agric Food Chem 56: 7700-7709 https://doi.org/10.1021/jf801668c
  60. Won SH, Kwon KR, Rhim TJ, Kim DH. 2008. The effect of Crataegi Fructus pharmacopuncture on adipocyte metabolism. J Pharmacopuncture 11: 63-73 https://doi.org/10.3831/KPI.2008.11.2.063
  61. Yu MH, Im HG, Lee HJ, Ji YJ, Lee IS. 2006. Components and their antioxidative activities of methanol extracts from sarcocarp and seed of zizyphus jujuba var. inermis rehder. Korean J Food Sci Technol 38: 128-134
  62. Yu SY, Lee YJ, Kang SN, Lee SK, Jang JY, Lee HK, Lim JH, Lee OH. 2013. Analysis of food components of Carthamus Tinctorius L. seed and its antimicrobial activity. Korean J Food Preserv 20: 227-233 https://doi.org/10.11002/kjfp.2013.20.2.227

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