Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antioxidant Activities of Rubus coreanus Miquel and Morus alba L. Fruits

복분자와 오디의 항산화 특성

  • Jun, Hyun-Il (Dept. of Food Science and Technology, Chonbuk National University) ;
  • Kim, Young-Ae (R&D Center, Daesang FNF Co., Ltd.) ;
  • Kim, Young-Soo (Dept. of Food Science and Technology, Chonbuk National University)
  • 전현일 (전북대학교 식품공학과) ;
  • 김영애 (대상 FNF(주) 한국식 신선연구소) ;
  • 김영수 (전북대학교 식품공학과)
  • Received : 2013.11.18
  • Accepted : 2014.01.28
  • Published : 2014.03.31
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Abstract

The antioxidant activities of extracts from Rubus coreanus Miquel (black raspberry) and Morus alba L. (mulberry) fruits were investigated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, 2,2-azino-bis-(3-ethylbenzo-thiazoline-6-sulfonic acid) (ABTS) assay, and reducing power assay. Aqueous mixtures of ethanol, methanol, and acetone were analyzed in order to determine the most effective extraction solvent for the two fruits. Black raspberry and mulberry extracts with the 60:40 acetone-water mixtures (v/v) showed the highest DPPH radical scavenging activities (56.2 and 85.2%, respectively) compared to the other extraction solvents. The 60% acetone extract was finally selected as an extraction solvent and then sequentially fractionated according to solvent polarity. Among the fractions of the two fruits, the ethyl acetate fraction showed the highest antioxidant activity as well as total phenolic and total flavonoid contents. In addition, there were high correlation coefficients between antioxidant activities and their contents. The $EC_{50}$ value of the ethyl acetate fraction from mulberry fruit was 2.2 times lower than that of butylated hydroxytoluene (BHT) in DPPH assay. The major phenolic acid and anthocyanin of the two fruits were protocatechuic acid and cyanidin-3-glucoside, respectively.

다양한 추출용매를 사용하여 얻은 복분자와 오디의 추출용액 중에서 가장 높은 항산화 활성을 보인 60% acetone과 이들의 분획물을 대상으로 측정한 항산화 활성에서 시료별로는 오디, 분획별로는 ethyl acetate 분획의 $EC_{50}$ 값이 낮게 나타났다. 복분자와 오디의 항산화 성분 중 총 페놀성 화합물과 총 플라보노이드 함량이 안토시아닌 함량에 비해 매우 높게 나타났다. 항산화 성분과 항산화 활성의 상관성은 총 페놀성 화합물과 총 플라보노이드에서는 높은 상관성을 나타냈으나 안토시아닌에서는 오디에서만 높은 상관성을 나타내었다. 복분자와 오디의 주된 phenolic acid는 protocatechuic acid, p-hydrobenzoic acid 및 salicylic acid이었으며 주된 안토시아닌은 cyanidin-3-glucoside이었다. 위의 결과들을 종합해보면 복분자와 오디의 항산화 성분은 추출에 사용된 용매의 종류에 따라 함량 및 조성이 변화하며 그 결과로서 항산화 활성에 영향을 주게 됨을 확인할 수 있었다. 또한 오디 ethyl acetate 분획물은 DPPH assay와 reducing power에서 BHT보다 각각 2.2와 1.2배 낮은 $EC_{50}$ 값을 보여 천연 항산화 소재로서의 가능성을 보였다.

Keywords

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