The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effects of steamed Polygonatum odoratum extract on inhibition of adipocyte differentiation and lowing lipid in 3T3-L1 adipocytes

증자 둥굴레 추출물의 3T3-L1 지방세포에서 분화억제 및 지질강하 효과

  • Kang, Byung Tae (Dept. of Food Nutrition, Gimcheon University) ;
  • Choe, Won Kyung (Dept. of Food Nutrition, Gimcheon University) ;
  • Park, Dong Cheol (Dept. of Hotel Cuisine & Food Service Management, Gimcheon University) ;
  • Kim, Jong Kuk (Dept. of Food and Food -Service Industry, Kyungpook National University) ;
  • Park, Mora (Dept. of Food and Food -Service Industry, Kyungpook National University) ;
  • Kim, Sung Ok (Team for Scientification of Korean Medical Intervention (BK21 Plus) & Dept. of Herbal Pharmacology, Coll. of Oriental Medicine, Daegu Haany Univ.) ;
  • Kim, Mi Ryeo (Team for Scientification of Korean Medical Intervention (BK21 Plus) & Dept. of Herbal Pharmacology, Coll. of Oriental Medicine, Daegu Haany Univ.)
  • 강병태 (김천대학교 식품영양학과) ;
  • 최원경 (김천대학교 식품영양학과) ;
  • 박동철 (김천대학교 호텔조리외식경영학과) ;
  • 김종국 (경북대학교 과학기술대학 식품외식산업학과) ;
  • 박모라 (경북대학교 과학기술대학 식품외식산업학과) ;
  • 김성옥 (한의치료 기술과학화 사업팀(BK 21 Plus) & 대구한의대학교 한의과대학 본초약리학교실) ;
  • 김미려 (한의치료 기술과학화 사업팀(BK 21 Plus) & 대구한의대학교 한의과대학 본초약리학교실)
  • Received : 2014.02.21
  • Accepted : 2014.03.13
  • Published : 2014.03.30
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Abstract

Objectives : The purpose of this study was to investigate inhibitory effects of steamed Polygonatum odoratum extract (POE) on differentiation and adipogenesis in 3T3-L1 adipocytes. Methods : Polygonatum odoratum (P. odoratum) extract was extracted with ethyl acetate. Total phenolic and flavonoid contents in POE were measured for antioxidant activity. The spectrophotometric method was used to determine the DPPH and ABTS radical scavenging activity and ferric-reducing antioxidant potential (FRAP). MTT assay was examined for cell toxicity, oil red O staining was performed for intracelluar adipogenesis in differentiated 3T3-L1 adipocytes. Western blot analysis for measurement of CCAAT/enhancer-binding protein ${\alpha}$ ($C/EBP{\alpha}$), peroxisome proliferator-activated receptor${\gamma}$ ($PPAR{\gamma}$) and AMP-activated protein kinase (AMPK) expressions were performed. Results : The results revealed that POE has antioxidant activities. Contents of total polyphenolics and flavonoids were $50.83{\pm}1.52$ GAE mg/100g dry weight of POE and $17.05{\pm}2.47$ RE mg/100g dry weight of POE, respectively. DPPH radical scavenging activity, and FRAP in 10 mg/ml concentration were $92.1{\pm}0.6%$, $244.8{\pm}9.0{\mu}M$ Fe(II) and ABTS inhibition in 5 mg/ml concentration was $84.8{\pm}4.1%$. Treatment of POE in adipocytes inhibited the differentiation and adipogenesis of 3T3-L1 adipocytes compared to those of vehicle control. Additionally, protein expressions of $C/EBP{\alpha}$ and $PPAR{\gamma}$, major transcription factor for the adipogenic genes, were significantly decreased compared to those of vehicle control (p<0.05). Futhermore, phosphorylation of AMPK was increased in 3T3-L1 adipocytes treated with POE compared to that of vehicle control (p<0.05). Conclusions : we demonstrate that steamed P. odoratum extract (POE) has potentiating antioxidant activities, inhibits differentiation and lipid accumulation and also induces energy expenditure in adipocytes, which may contribute to antiobesity property.

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