DOI QR코드

DOI QR Code

Antioxidant, angiotensinconverting enzyme and xanthin oxidase inhibitory activity of extracts from Saururus chinensis leaves by ultrafine grinding

초미세 분쇄한 삼백초(Saururus chinensis) 추출물의 항산화, angiotensinconverting enzyme 및 xanthin oxidase 억제 활성

  • Cho, Young-Je (School of Food science and Biotechnology, Food and Bio-Industry Research Institute, Kyungpook National University)
  • 조영제 (경북대학교 식품공학부, 식품생물산업연구소)
  • Received : 2013.07.25
  • Accepted : 2013.10.18
  • Published : 2014.02.28

Abstract

In this study, the biological activity of water and ethanol extracts from Saururus chinensis by ultra-fine grinding for functional food source are examined. It is more effective to use ethanol than water when extracting phenolic compounds. Approximately 2.5 times higher extraction yield were shown when it was ultra-fine grinded because the particle size decreases, thereby increasing the extraction yield. Normal grinded sample extracts showed 69.8% of DPPH inhibition effect, while fine grinded and ultra-fine grinded sample extracts showed 70.7% and 83.8% each, respectively. Normal extract, as well as fine grinded and ultra-fine grinded extracts, showed over 97% of ABTS inhibition effect, thereby indicating only a slight difference in the anti-oxidative activity with the grinding method. Higher PF was determined with fine grinded and ultra-fine grinded extracts than the normal grinded extract, while ultra-fine grinded 50% ethanol extracts showed the highest anti-oxidative activity value of 1.8 PF. The fine grinded and ultra-fine grinded particle sizes are smaller than the normal grinded particle size, thus increasing the inhibition rate of the TBARS. Furthermore, the ethanol extract was revealed to have a higher effect than the water extracts. The xanthin oxidase inhibition, on the other hand, was identified as ultra-fine grinded that led to the increase in the enzyme inhibition effect. In the angiotensin-converting enzyme, water extracts with normal grinding did not show inhibition activity, while 50% ethanol extracts showed 24% inhibition activity. Moreover, the ethanol extracts showed higher inhibition effect compared to the water extracts. Ultra-fine grinded 50% ethanol extracts showed a slight antibacterial effect on the Staphylococcus aureus and Escherichia coli, while the other extracts showed none. The result suggests that Saururus chinensis extracts by ultra-fine grinding may be more useful than normal grinding as potential sources due to anti-oxidation, angiotensin converting enzyme and xanthine oxidase inhibition.

초미세 분쇄를 하였을 때 입자가 작아질수록 추출수율이 높아져 약 2.5배 높은 추출수율을 나타내었다. 일반 분쇄한 시료 추출물에서 69.8%의 전자공여능 억제효과가 관찰되었고, 미세분쇄와 초미세분쇄 추출물에서는 각각 70.7과 83.8%의 억제효과를 나타내었다. 일반분쇄 추출물과 미세분쇄 및 초미세분쇄 후 추출물 모두 97% 이상의 높은 ABTS 억제효과를 나타내어 분쇄 방법에 따른 항산화력의 차이는 거의 없었다. 일반 분쇄한 시료 추출물 보다 미세분쇄와 초미세분쇄 추출물에서 더 높은 PF값을 확인하였으며, 50% ethanol 초미세분쇄 추출물에서 1.8 PF로 가장 높은 항산화력을 나타내었다. 미세분쇄와 초미세분쇄 추출물에서는 일반분쇄 추출물에 비해 입자크기가 작아질수록 TBARS 억제율이 높아지며, 물 추출물보다 ethanol 추출물의 효과가 더 우수한 것으로 확인되었다. Xanthin oxidase 저해의 경우 초미세분쇄 후 효소억제 증대 효과를 확인할 수 있었다. Angiotensin converting enzyme 억제활성은 일반분쇄 추출의 경우 물 추출물에서는 억제활성이 나타나지 않았고, 50% ethanol 추출물에서 24%의 억제율이 확인되었다. 또한, ethanol 추출물의 억제효과가 물 추출물에 비해 상대적으로 우수하였다. 50% ethanol 초미세분쇄 추출물에서 Staphylococcus aureus, Escherichia coli에 대해서 아주 약한 항균효과를 나타내었을 뿐 나머지 추출물에서의 항균효과는 거의 관찰되지 않았다.

Keywords

References

  1. Lee ST, Park JM, Lee HK, Kim MB, Cho JS, Heo JS (2000) Component comparison in different growth stages and organs of Saururus chinensis Bail. Korean J Med Crop Sci, 8, 312-318
  2. Kim SK, Kim YH, Kang DK, Chung SH, Lee SP, Lee SC (1998) Essential oil content and composition of aromatic constituents on leaf of Saururus chinensis, Angelica dahuriea and Cinidium officiane. Korea J Med Crop Sci, 6, 299-304
  3. Choe KH, Yoon CH, Kwon SJ (1994) A study on chemical composition of Saururaceac growing in Korean on flavonoid constituents of Saururus chinensis. J Korean Soc analytic Sci, 7, 11-15
  4. Lee ST, Lee YH, Choi YJ, Lee YH, Choi JS, Heo JS (2001) Yield and bioactive component on different compostamounts and culture method of Saururus chinensis Bail. Korean J Med Crop Sci, 9, 220-224
  5. Kim BH, Song WS (2000) The dyeability and antimicrobial activity of Saururus chinensis (I). J Korean Home Econom, 38, 1-9
  6. Lim DK, Choi U, Shin DH (1996) Antioxidantive activity of ethanol extract from Korean medicinal plants. Korean J Food Sci Technol, 28, 83-89
  7. Park SS, Yu KH, Min TJ (1998) Antioxidantive activity of ethanol extract from fruiting bodies of mushrooms. Korean J Mycology, 26, 69-77
  8. Lee JM, Son ES, Oh SS, Han DS (2001) Contents of total flavonoid and biological activiries of edible plants. Korean J Diet Cul, 16, 504-514
  9. Kim NM, Ko SR, Choi KJ, Kim WJ (1993) Effect of some factors on extraction of effectual componenets in cinnamon extract. J Korean Agric Chem Soc, 36, 17-22
  10. Bell LN (2001) Stability testing of nutraceuticals and functional foods. In : Handbook of nutraceuticals and functional foods. Wildman REC(ed). CRC press, New York, p 501-516
  11. Lopez-Rubino A, Gavara R, Lagaron JM (2006) Bioactive packaging: turning foods into healthier foods through biomaterials. Trends Food Sci Technol, 17, 567-575 https://doi.org/10.1016/j.tifs.2006.04.012
  12. Kim KH, Lee IH, Lee HS, Park JK (2003) R&D trend and information analysis of nanoparticles. Pros Ind Chem, 6, 46-61
  13. Kim CS, Kim CS, Kim HI (2009) Physicochemical properties of non-waxy rice flour affected by grinding methods and steeping times. J Korean Soc Food Sci Nutr, 38, 1076-1083 https://doi.org/10.3746/jkfn.2009.38.8.1076
  14. Kim JS, Lee JY, Park KT, An BJ, Lee SH, Cho YJ (2013) The biological activity from Prunella vulgaris extracts. Korean J Food Preserv, 20, 234-241 https://doi.org/10.11002/kjfp.2013.20.2.234
  15. Blois MS (1958) Antioxidant determination by the use of a stable free radical. Nature, 26, 1199-1200
  16. Pellegrin N, Roberta R, Min Y, Catherine RE (1998) Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2,2'-Azinobis(3-ethylene benzothiazoline-6-sulfonic acid)radical cation decolorization assay. Method Enzymol, 299, 379-389
  17. Andarwulan N, Shetty K (1999) Phenolic content in differentiated tissue cultures of untansformed and Ahrobacterium-transformed roots of anise (Pimpinella anisum L.). J Agric Food Chem, 47, 1776-1780 https://doi.org/10.1021/jf981214r
  18. Buege JA, Aust SD (1978) Microsomal lipid peroxidation. Method Enzymol, 105, 302-310
  19. Cushman DW, Cheung HS (1971) Spectrophotometric assay and properties of the angiotensin- converting enzyme of rabbit lung. Biochem Pharmacol, 20, 1637-1648 https://doi.org/10.1016/0006-2952(71)90292-9
  20. Stirpe F, Corte ED (1969) The regulation of rat liver xanthin oxidase. J Biol Chem, 244, 3855-3862
  21. Gavidson PH, Parish ME (1989) Methods for testing the efficacy of food antimicrobials. J Food Technol, 43, 148-154
  22. Stevenson TH, Lucia LM, Acuff GR (2000) Development of selective medium for isolation of Helicobacter pylori from cattle and beef samples. Appl Environ Micobiol, 66, 723-727 https://doi.org/10.1128/AEM.66.2.723-727.2000
  23. Choi HS, Kim MG, Shin JJ, Pack JM, Lee JS (2003) The antioxidant activities of the some commercial teas. J Korean Soc Food Sci Nutr, 32, 723-727 https://doi.org/10.3746/jkfn.2003.32.5.723
  24. Han MR, Chang MJ, Kim MH (2007) Investigation of physical property change in modified rice starch by ultra fine pulverization. J Korean Soc Appl Biol Chem, 50, 160-166
  25. Han MR, Kim AJ, Chang MJ, Lee SJ, Kim HS, Kim MH (2009) Investigation of physical property change in modified corn starch by ultra fine pulverization. Food Bioeng Progress, 13, 335-340
  26. Shin HL (2003) Biological activity of phenol compound from mulberry fruits. MS Thesis, Sangju National University, Korea.
  27. Aoshima H, Tsumoue H, Koda H, Kiso Y (2004) Aging of whiskey increases 1,1- diphenyl-2-picrylhydrazyl radical scavenging activity. J Agric Food Chem, 52, 5240-5244 https://doi.org/10.1021/jf049817s
  28. Cho YJ, Ju IS, Chun SS, An BJ, Kim JH, Kim MU, Kwon OJ (2008) Screening of biological activities of extracts from Rhododendron mucronulatum Turcz. flowers. J Korean Soc Food Sci Nutr, 37, 276-281 https://doi.org/10.3746/jkfn.2008.37.3.276
  29. Noro T, Fukushima S (1988) Inhibitors of xanthine oxidase from the flowers and buds of Daphne genkwa. Chem Pharm Bull, 31, 3984-3988
  30. Miyoshi D, Richard LS (1975) Pulmonary angiotensin converting enzyme. J Biol Chem, 250, 6762-6768
  31. Funayama S, Hikono H (1979) Hypotensive principles of Diospyors kaki Leaves. Chem Pharm Bull, 27, 2865-2871 https://doi.org/10.1248/cpb.27.2865
  32. Stewart JM, Ferreira SH, Greene LJ (1971) An inhibitors of the pulmonary inactivation of bradykinin and conversion of angiotensin I to II. Biochem Pharmacol, 20, 157-163
  33. Kameda K, Takaku T, Okyada H, Kimyra S (1987) Inhibitory effects of various flavonoids isolated from leaves of persimmon on angiotensin-converting enzyme activity. J Natural products, 50, 680-686 https://doi.org/10.1021/np50052a017

Cited by

  1. Hyaluronidase inhibitory activity of extracted phenolic compounds from ultrafine grind Saururus chinensis vol.23, pp.1, 2016, https://doi.org/10.11002/kjfp.2016.23.1.20
  2. Physicochemical Characteristics and Antioxidant Activity of Bracken (Pteridium aquilinum Kuhn) in Namhae vol.31, pp.3, 2015, https://doi.org/10.9724/kfcs.2015.31.3.288
  3. Antimicrobial and Antioxidant Activities of Ethanol Leaf Extract of Dendropanax morbiferus Lev. vol.31, pp.5, 2015, https://doi.org/10.9724/kfcs.2015.31.5.515
  4. Changes in Extraction Efficiency of Pine Needles depending on Extraction Method and the Condition vol.48, pp.1, 2016, https://doi.org/10.7584/ktappi.2016.48.1.093
  5. 초미세 분쇄한 삼백초(Saurus chinensis) 추출물의 미용 활성 vol.23, pp.6, 2014, https://doi.org/10.11002/kjfp.2016.23.6.899
  6. 초미세 분쇄한 감국으로부터 추출된 phenolic 화합물의 xanthine oxidase 저해 효과 vol.27, pp.8, 2014, https://doi.org/10.5352/jls.2017.27.8.902
  7. 덖음온도를 달리하여 전저리한 삼백초 건조물의 이화학적 특성 및 물과 70% 에탄올 추출물의 항산화효과 vol.52, pp.4, 2019, https://doi.org/10.4163/jnh.2019.52.4.399
  8. Effect of Roasting Time and Cryogenic Milling on the Physicochemical Characteristics of Dried Ginseng Powder vol.9, pp.2, 2014, https://doi.org/10.3390/foods9020223
  9. Elicitor 처리한 주름조개풀(Oplismenus undulatifolius)의 항산화, tyrosinase, elastase, collagenase 및 hyaluronidase 억제 활성 증대 vol.63, pp.3, 2014, https://doi.org/10.3839/jabc.2020.030