Antioxidant and Cytotoxic Activity of Compounds from the Stem of Juncus effusus

등심초로부터 분리된 화합물의 항산화 활성 및 암세포 성장 억제 효과

The stems of Juncus effusus were extracted with 70% aqueous ethanol and the concentrated extract was partitioned with ethyl acetate, n-butanol and $H_2O$, successively. Two compounds were isolated from the ethyl acetate fraction through the repeated silica gel and Sephadex LH-20 column chromatographies. According to the results of physico-chemical and spectroscopic data including NMR and IR, the chemical structures of the compounds were determined as dehydroeffusol (1) and effusol (2). Dehydroeffusol and effusol exhibited potent scavenging activity for DPPH and ABTS radicals with the $IC_{50}$ values as $130{\pm}3.21$ and $79{\pm}1.53{\mu}M$ in DPPH assay, and as $39{\pm}3.51$ and $24{\pm}2.73{\mu}M$ in ABTS assay, respectively. The compounds also significantly inhibited the proliferation of human cancer cell lines, AGS and A549.

등심초(Juncus effusus)를 70% ethanol로 추출하고 이 추출물을 ethyl acetate분획, n-butanol 및 $H_2O$ 분획으로 나누었다. Ethyl acetate 분획에 대하여 silica gel, Sephadex LH-20 column chromatography를 반복 실시하여 2종의 화합물을 분리하였다. 화합물 1 (dehydroeffusol)과 2 (effusol)에 대하여 free radical 소거활성을 측정한 결과, dehydroeffusol (DPPH; $130{\pm}3.21{\mu}M$, ABTS; $39{\pm}3.51{\mu}M$)과 effusol (DPPH; $79{\pm}1.53{\mu}M$, ABTS; $24{\pm}2.73{\mu}M$)은 강한 항산화 활성을 나타내었다. 또한 이들 화합물들의 인체유래 위암세포(AGS), 폐암세포(A549)에 대한 성장억제 효과를 측정한 결과 모두 항암약물(CPA)과 유사하거나 더 높은 성장 저해 효과를 보였다.