Changing Temperature Affects Anesthetic Effects and Physiological Stress Responses in Marine Medaka, Oryzias dancena

해산송사리, Oryzias dancena의 수온 변화에 의한 마취 효과와 생리적 스트레스 반응

  • Received : 2014.01.29
  • Accepted : 2014.03.07
  • Published : 2014.04.30


본 연구는 온도 변화에 따른 해산송사리, Oryzias dancena의 마취 효과 및 생리적 스트레스 반응을 평가하고, 고온 및 저온에서 최적의 마취 수온을 구명하였다. 해산송사리에서 고온 실험($36{\sim}42^{\circ}C$)과 저온 실험($4{\sim}10^{\circ}C$)을 통해 마취 효과를 조사하였으며, 각 마취수온에서 전어체의 cortisol과 glucose를 측정하였다. 각 수온 마취 실험 후, 전개체는 모두 생존하였으며, 해산송사리의 마취시간은 고온 실험에서 고온 일수록, 저온 실험에서는 저온 일수록 유의적으로 빨랐다(P<0.05). 회복 시간은 유의하게 고온 실험에서 수온이 감소할수록, 저온 실험에서는 수온이 증가할수록 유의적으로 느렸다(P<0.05). 아가미 운동수는, 고온 실험에서 수온이 증가함에 따라 빨랐으며, 저온 실험에서는 수온이 감소할때 빨랐다(P<0.05). 수온이 $38^{\circ}C$$8^{\circ}C$ 일때의 마취 조건에서 실험직후 전어체 cortisol이 최대치로, 실험후 6시간까지 점진적으로 감소한 반면, 전어체 glucose는 실험후 1시간에 최고치를 보이고 실험후 2시간까지는 감소하였다. 기존의 마취제를 쓰지 않은 본 연구로 인해, 해산송사리의 여타 연구에서 샘플 및 사용자가 보다 더 안전하고 용이한 샘플 취급이 가능할 것으로 사료된다.



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