Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지
DOI QR코드

DOI QR Code

Protective Effects of Fucoidan against Acute Alcohol-induced Liver Injury in Rats

후코이단이 쥐의 급성 알콜성 간손상에 미치는 영향

  • Kim, Mi-Ja (College of Biotechnology and Bioengineering Bioengineering, Sungkyunkwan University) ;
  • Jeon, Joseph (Haewon Biotech, Inc.) ;
  • Lee, Sung Pyo (Misuba RTech Co., Ltd.) ;
  • Lee, Jin-Sil (Department of Foodservice Management and Nutrition, Sangmyung University)
  • Received : 2013.10.03
  • Accepted : 2013.12.17
  • Published : 2014.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study was to investigate the protective effects of fucoidan on acute alcohol-induced liver injury in rats. Experimental animals were randomly divided into four groups: (1) a control group, (2) a group fed 25% ethanol, (3) a group fed 25% ethanol and 250 mg/kg BW of fucoidan (25% ethanol+FUCO250), and (4) a group fed 25% ethanol and 500 mg/kg BW of fucoidan (25% ethanol+FUCO500). Each group was fed orally two times per day for 15 days. Liver weights in the 25% ethanol group were increased compared to the control group, while liver weights in the 25% ethanol+FUCO500 group were significantly decreased compared to the 25% ethanol group (p<0.05). Plasma concentrations of triglyceride, cholesterol, and LDL-cholesterol were elevated in the 25% ethanol group; however, these levels in the 25% ethanol+FUCO250 group were significantly decreased compared to the 25% ethanol group (p<0.05). The glutamic-pyruvic transaminase activity of the 25% ethanol+FUCO500 group also was significantly lower than the 25% ethanol group (p<0.05). These results indicate that fucoidan might protect against acute alcohol-induced liver injury.

본 연구는 해조류에 존재하는 황산기를 함유하고 있는 후코이단의 급성 알코올 간 손상 모델에서의 간 보호능을 관찰하였다. 실험 결과 후코이단은 급성으로 알코올을 섭취시킨 쥐의 체중감소를 억제시켰으며, 알코올 섭취를 통해 나타날 수 있는 중성지질, 콜레스테롤, LDL-콜레스테롤 상승을 유의적으로 감소시킨 것으로 나타났다. 또한 간 기능을 대표하는 혈중의 GOT는 유의적인 차이는 없었으나, GPT는 유의적으로 후코이단 경구투여에 의해 감소되었다. 또한 후코이단은 지방이 비대해 지는 현상과 간세포의 지방축척을 감소시킨 것으로 관찰되었다. 따라서 이러한 결과로 보아 후코이단은 급성 알코올 섭취에 의한 간손상을 완화시켜 줄 수 있는 식품소재로 활용가능하다고 사료된다.

Keywords

References

  1. SK. Annual report on the cause of death statistics. Statistics Korea, Daejeon, Korea. pp. 1-231 (2012)
  2. MHW. OECD health data 2012. Kyungsung Press, Seoul, Korea. pp. 1-123 (2012)
  3. Chevolot L, Foucault A, Chaubet F, Kervarec N, Sinquin C, Fisher AM, Boisson-Vidal C. Further data on the structure of brown seaweed fucans: Relationships with anticoagulant activity. Carbohyd. Res. 319: 154-165 (1999) https://doi.org/10.1016/S0008-6215(99)00127-5
  4. Cumashi A, Ushakova NA, Preobrazhenskaya ME, D'Incecco A, Piccoli A, Totani L, Tinari N, Morozevich GE, Berman AE, Bilan MI, Usov AI, Ustyuzhanina NE, Grachev AA, Sanderson CJ, Kelly M, Rabinovich GA, Iacobelli S, Nifantiev NE. A comparative study of the anti-inflammatory, anticoagulant, antiangiogenic, and antiadhesive activities of nine different fucoidans from brown seaweeds. Glycobiology 17: 541-552 (2007)
  5. Hayashi K, Nakano T, Hashimoto M, Kanekiyo K, Hayashi T. Defensive effects of a fucoidan from brown alga Undaria pinnatifida against herpes simplex virus infection. Int. Immunopharmacol. 8: 109-116 (2008) https://doi.org/10.1016/j.intimp.2007.10.017
  6. Usui T. Isolation of highly purified fucoidan from Eisenia bicyclics and its anticoagulant and antitumor activities. Agr. Biol. Chem. Tokyo 44: 1965-1966 (1980) https://doi.org/10.1271/bbb1961.44.1965
  7. Liu JM, Bignon J, Haroun-Bouhedja F, Bittoun P, Vassy J, Fermandjian S, Wdzieczak-Bakala J, Boisson-Vidal C. Inhibitory effect of fucoidan on the adhesion of adenocarcinoma cells to fibronectin. Anticancer Res. 25: 2129-2133 (2005)
  8. Wang WT, Zhou JH, Xing ST, Guan HS. Immunomodulating action of marine algae sulfated polysaccharides on normal and immunosuppressed mice. Chin. J. Pharm. Toxicol. 8: 199-202 (1994)
  9. Zhang QB, Yu PZ, Zhou GF, Li ZE, Xu ZH. Studies on antioxidant activities of fucoidan from Laminaria japonica. Chin. Trad. Herbal Drugs 34: 824-826 (2003)
  10. Shibata H, Kimura-Takagi I, Nagaoka M, Hashimoto S, Aiyama R, Iha M, Ueyama S, Yokokura T. Properties of fucoidan from Cladosiphon okamuranus tokida in gastric mucosal protection. Biofactors 11: 235-245 (2000) https://doi.org/10.1002/biof.5520110402
  11. Saito A, Yoneda M, Yokohama S, Okada M, Haneda M, Nakamura K. Fucoidan prevents concanavalin A-induced liver injury through induction of endogenous IL-10 in mice. Hepatol. Res. 35: 190-198 (2006)
  12. Hayashi S, Itoh A, Isoda K, Kondoh M, Kawase M, Yagi K. Fucoidan partly prevents CCl4-induced liver fibrosis. Eur. J. Pharmacol. 580: 380-384 (2008) https://doi.org/10.1016/j.ejphar.2007.11.015
  13. Kang KS, Kim ID, Kwon RH, Lee JY, Kang JS, Ha BJ. The effects of fucoidan extracts on CCl(4)-induced liver injury. Arch. Pharm. Res. 31: 622-627 (2008) https://doi.org/10.1007/s12272-001-1203-8
  14. Kim MJ, Chang UJ, Lee JS. Inhibitory effects of fucoidan in 3T3-L1 adipocyte differentiation. Mar. Biotechnol. 11: 557-562 (2009) https://doi.org/10.1007/s10126-008-9170-1
  15. Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin. Chem. 18: 499-502 (1972)
  16. Kim MJ, Kim HK. Effect of garlic on high fat induced obesity. Acta Biol. Hung. 62: 244-254 (2011) https://doi.org/10.1556/ABiol.62.2011.3.4
  17. Chung H-J, Jeun J, Houng S-J, Jun H-J, Kweon D-K, Lee S-J. Toxicological evaluation of fucoidan from Undaria pinnatifida in vitro and in vivo. Phytother. Res. 24: 1078-1083 (2010)
  18. Nam CS, Kang KS, Ha BJ. Ulva alctuca fucoidan extract and its protective effects on CCl4-induced liver dysfunction. Korean J. Biotechnol. Bioeng. 22: 73-77 (2007)
  19. Park SY, Kim DS, Kang S, Park S. Hepatoprotective effects of pig placental hydrolysates on liver damage induced rats by injecting carbon tetrachloride. J. Appl. Biol. Chem. 55: 109-115 (2012) https://doi.org/10.3839/jabc.2011.067
  20. Yang S-Y, Kang J-H , Lee K-W. Protective effect of functional perilla frutescens hot-water extract against tert-butyl hydroperoxide- induced liver oxidative damage in rats. J. Fd. Hyg. Safety 28: 146-151 (2013) https://doi.org/10.13103/JFHS.2013.28.2.146
  21. Jo W-S, Nam B-H, Oh S-J, Choi Y-J, Kang E-Y, Hong S-H, Lee S-H, Jeong MH. Hepatic protective effect and single-dose toxicity study of water extract of Cordyceps militaris grown upon Protaetia dreujtarsis. Korean J. Food Sci. Technol. 40: 1-5 (2008)
  22. Lee E-H, Chyun J-H. Effects of Chongkukjang intake on lipid metabolism and liver function in alcoholic fatty liver rats. J. Korean Soc. Food Sci. Nutr. 38: 1506-1515 (2009) https://doi.org/10.3746/jkfn.2009.38.11.1506
  23. Oh SH, Cha YS, Choi DS. Effects of Angelica gigas Nakai diet on lipid metabolism alcohol metabolism and liver function of rats administered with chronic ethanol. J. Korean Soc. Agric. Chem. Biotechnol. 42: 29-33 (1999)
  24. Lee JH, Park KS. Effect of ganoderma lucidum on the liver function and lipid metabolism in alcohol-consuming rats. Korean J. Nutr. 32: 519-525 (1999)
  25. Sohn HS, Jung BM, Cha YS. Effects of Ixeris Sonchifola H. Fiet on lipid metabolism and liver function of rats administered with ethanol. Korean J. Nutr. 34: 493-498 (2001)
  26. Kim MJ, Jeon J, Lee JS. Fucoidan prevents high-fat diet-induced obesity in animals by suppression of fat accumulation. Phytother. Res. 28: 137-143 (2014) https://doi.org/10.1002/ptr.4965
  27. Rouach H, Clement M, Ofanelli MT, Janvier B, Nordmann J, Nordmann R. Hepatic lipid peroxidation and mitochondria susceptibility to peroxidative attacks during ethanol inhalation and withdrawal. Biochim. Biophys. Acta 753: 439-444 (1983) https://doi.org/10.1016/0005-2760(83)90068-1
  28. Huang L, Wen K, Gao X, Liu Y. Hypolipidemic effect of fucoidan from Laminaria japonica in hyperlipidemic rats. Pharm. Biol. 48: 422-426 (2010) https://doi.org/10.3109/13880200903150435
  29. Mori N, Nakasone K, Tomimori K, Ishikawa C. Beneficial effects of fucoidan in patients with chronic hepatitis C virus infection. World J. Gastroentero. 18: 2225-2230 (2012) https://doi.org/10.3748/wjg.v18.i18.2225
  30. Lefkowitch JH. Morphology of alcoholic liver disease. Clin. Liver Dis. 9: 37-53 (2005) https://doi.org/10.1016/j.cld.2004.11.001
  31. Crawford JM. Histologic findings in alcoholic liver disease. Clin. Liver Dis. 16: 699-716 (2012) https://doi.org/10.1016/j.cld.2012.08.004
  32. Soeda S, Sakaguchi S, Shimeno H, Nagamatsu A. Fibrinolytic and anticoagulant activities of highly sulfated fucoidan. Biochem. Pharmacol. 43: 1853-1858 (1992) https://doi.org/10.1016/0006-2952(92)90721-T

Cited by

  1. Effects of Cladosiphon okamuranus on Lipid Metabolism in High-fat-diet Rats vol.26, pp.6, 2016, https://doi.org/10.5352/JLS.2016.26.6.657