Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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The Antiviral Effects of Areca catechu L. Extract

빈랑 추출물의 새로운 항바이러스 활성

  • Lee, Doseung (Jeju Biodiversity Research Institute (JBRI), Jeju Technopark) ;
  • Boo, Kyung Hwan (Subtropical Horticulture Research Institute, Jeju National University) ;
  • Kim, Young Cheon (Subtropical Horticulture Research Institute, Jeju National University) ;
  • Lee, Jin-Man (Department of Food & Biotechnology, Hoseo University) ;
  • Kang, Seungtae (College of Applied Life Science (SARI), Jeju National University) ;
  • Lee, Wang Shik (College of Applied Life Science (SARI), Jeju National University) ;
  • Riu, Key Zung (College of Applied Life Science (SARI), Jeju National University) ;
  • Lee, Dong-Sun (College of Applied Life Science (SARI), Jeju National University)
  • 이도승 ((재)제주테크노파크 생물종다양성연구소) ;
  • 부경환 (제주대학교 아열대원예산업연구소) ;
  • 김영천 (제주대학교 아열대원예산업연구소) ;
  • 이진만 (호서대학교 식품공학과) ;
  • 강승태 (제주대학교 생명공학부) ;
  • 이왕식 (제주대학교 생명공학부) ;
  • 류기중 (제주대학교 생명공학부) ;
  • 이동선 (제주대학교 생명공학부)
  • Received : 2013.11.26
  • Accepted : 2014.01.29
  • Published : 2014.04.30
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Abstract

Trafficking of viral glycoproteins to the cell surface results in syncytium formation in baby hamster kidney (BHK) cells infected with Newcastle disease virus (NDV). An extract from the medicinal Areca catechu L plant inhibited not only syncytium formation, but also trafficking of the hemagglutinin-neuramidase (HN) glycoprotein to the cell-surface. The viral glycoprotein was processed within the endoplasmic reticulum during transit to the cell membrane. Fungal extracts showed inhibitory activities ($IC_{50}10{\mu}g/mL$) against ${\alpha}$-glucosidase. These results suggested that A. catechu L. extracts inhibited the cell-surface expression of NDV-HN glycoprotein without significantly affecting HN glycoprotein synthesis in NDV-infected BHK cells.

Newcastle disease virus (NDV) 감염된 baby hamster kidney (BHK) 세포에서 합포체 형성은 세포막 표면으로의 수송된 바이러스 당단백질 hemagglutinin-neuramidase (HN)에 의해 일어난다. HAU 값은 추출물의 농도가 25.0과 $3.2{\mu}g/mL$ 사이에서는 현저하게 감소하였으나, NDV 감염된 HAD (%)는 $25.0{\mu}g/mL$ 농도에서 광범위한 흡착능의 감소를 나타내 바이러스 당단백질의 세포내 생합성은 저해되지 않았다. 그러므로, 약용식물인 빈랑 메탄올 추출물이 바이러스 당단백질의 세포막으로의 수송과 함께 합포체 형성을 저해하여 항바이러스 작용을 하였다. 또한 빈랑 추출물의 저해활성을 조사한 결과 ${\alpha}$-glucosidase에 대한 추출물의 $IC_{50}$은 $10.0{\mu}g/mL$이었으며, ${\alpha}$-mannosidase, ${\beta}$-glucosidase, ${\beta}$-mannosidase에 대한 빈랑 추출물의 $IC_{50}$은 각각 20.0, 40.0, $80.0{\mu}g/mL$로 나타나 ${\beta}$-type glycosidases 보다 ${\alpha}$-type glycosidases에 대한 효소활성 저해능이 우수하였다. 따라서 $IC_{50}$농도에서는 세포내에서 당단백질 생합성은 저해되지 않으며 당단백질의 수송을 저해하는 것으로 판단되었으며 향후 항바이러스 관련 작용기작의 연구가 필요하다고 판단된다.

Keywords

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