Extraction of Oil from Chlorella vulgaris Using Supercritical Carbon Dioxide and Organic Solvent

초임계 이산화탄소와 유기용매를 이용한 Chlorella vulgaris 오일의 추출

  • Ryu, Jong-Hoon (Department of Chemical Engineering, The University of Suwon) ;
  • Park, Mi-Ran (Department of Chemical Engineering, The University of Suwon) ;
  • Lim, Giobin (Department of Chemical Engineering, The University of Suwon)
  • Received : 2014.01.22
  • Accepted : 2014.03.17
  • Published : 2014.04.30


Three different types of extraction processes, which used supercritical carbon dioxide ($SCCO_2$) and organic solvent, were attempted to improve the extraction yield of oil from Chlorella vulgaris: cosolvent-modified $SCCO_2$ extraction, $SCCO_2$ extraction with ultrasonic sample treatment in organic solvent, and static extraction with organic solvent followed by dynamic $SCCO_2$ extraction. Among these, the last $SCCO_2$ extraction process was found to be most effective in the extraction of oil. Compared with pure $SCCO_2$ extraction, the extraction yield of oil was observed to increase about 7 times.


Supported by : 농촌진흥청


  1. Nigam, P. S. and A. Singh (2011) Production of liquid biofuels from renewable resources. Prog. Energy Comb. Sci. 37: 52-68.
  2. Singh, A., D. Pant, N. E. Korres, A. S. Nizami, S. Prasad, and J. D. Murphy (2010) Key issues in life cycle assessment of ethanol production from lignocellulosic biomass: challenges and perspectives. Bioresour. Technol. 101: 5003-5012.
  3. Singh, A., B. M. Smyth, and J. D. Murphy (2010) A biofuel strategy for Ireland with an emphasis on production of biomethane and minimization of land-take. Renew. Sustain. Energy Rev. 14: 277-288.
  4. Demirbas, A. (2009) Political, economic and environmental impacts of biofuels: A review. Appl. Energy 86: S108-S117.
  5. Zhu, L. D., E. Hiltunen, E. Antila, J. J. Zhong, Z. H. Yuan, and Z. M. Wang (2014) Microalgal biofuels: Flexible bioenergies for sustainable development. Renew. Sust. Energ. Rev. 30: 1035-1046.
  6. Findlater, K. M. and M. Kandlikar (2011) Land use and secondgeneration biofuel feedstocks: The unconsidered impacts of Jatropha biodiesel in Rajasthan, India. Energ. Poli. 39: 3404-3413.
  7. Spolaore, P., C. Joannis-Cassan, E. Duran, and A. Isambert (2006) Commercial applications of microalgae. J. Biosci. Bioeng. 101: 87-96.
  8. Patil, V., K. Q. Tran, and H. R. Giselrod (2008) Towards sustainable production of biofuels from microalgae. Int. J. Mol. Sci. 9: 1188-1195.
  9. Sharif Hossain, A. B. M., A. Salleh, A. N. Boyce, P. Chowdhury, and M. Naqiuddin (2008) Biodiesel fuel production from algae as renewable energy. Am. J. Biochem. Biotechnol. 4: 250-254.
  10. Bozan, B. and F. Temelli (2003) Extraction of poppy seed oil using supercritical $CO_2$. J. Food Sci. 68: 422-426.
  11. Salgin, U., O. Doker, and A. Calimli (2006) Extraction of sunflower oil with supercritical $CO_2$: Experiments and modeling. J. Supercrit. Fluids 38: 326-331.
  12. Mercer, P. and R. E. Armenta (2011) Developments in oil extraction from microalgae. Eur. J. Lipid Sci. Technol. 113: 539-547.
  13. Han, X., L. Cheng, R. Zhang, and J. Bi (2009) Extraction of safflower seed oil by supercritical $CO_2$. J. Food Eng. 92: 370-376.
  14. Macias-Sanchez, M. D., C. M. Serrano, M. R. Rodriguez, and E. M. de la Ossa (2009) Kinetics of the supercritical fluid extraction of carotenoids from microalgae with $CO_2$ and ethanol as cosolvent. Chem. Eng. J. 150: 104-113.
  15. Ryu, J. H., M. R. Park, and G. Lim (2011) Supercritical carbon dioxide extraction of oil from Chlorella vulgaris. KSBB J. 26: 453-458.
  16. Diaz-Reinoso, B., A. Moure, H. Domnguez, and J. C. Parajo (2006) Supercritical $CO_2$ extraction and purification of compounds with antioxidant activity. J. Agric. Food Chem. 54: 2441-2469.
  17. Valderrama, J. O., M. Perrut, and W. Majewski (2003) Extraction of astaxantine and phycocyanine from microalgae with supercritical carbon dioxide. J. Chem. Eng. Data 48: 827-830.
  18. Dunford, N. T. and F. Temelli (1995) Extraction of phospholipids from canola with supercritical carbon dioxide and ethanol. JAOCS 72: 1009-1015.
  19. Tang, S., C. Qin, H. Wang, S. Li, and S. Tian (2011) Study on supercritical extraction of lipids and enrichment of DHA from oilrich microalgae. J. Supercrit. Fluids 57: 44-49.
  20. Imahara, H., E. Minami, S. Hari, and S. Saka (2008) Thermal stability of biodiesel in supercritical methanol. Fuel 87: 1-6.
  21. Lee, S. B. and J. D. Lee (2009) The effect of ultrasonic energy on esterification of vegetable oil. J. Korean Ind. Eng. Chem. 20: 532-535.