한국가금학회지 (Korean Journal of Poultry Science)

  • 제41권1호
  • /
  • Pages.15-20
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  • 2014
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  • 1225-6625(pISSN)
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  • 2287-5387(eISSN)
한국가금학회 (The Korean Society of Poultry Science)
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DOI QR Code

재조합 가금 아데노바이러스 Fiber 2 단백질을 이용한 특이 난황 항체 생산

Production of Specific Egg Yolk Antibodies in Chicken against Recombinant Fowl Adenovirus Fiber 2 Protein

  • 정경민 (단국대학교 생명자원과학대학 동물자원학과) ;
  • 이성 (단국대학교 생명자원과학대학 동물자원학과) ;
  • 김정우 (단국대학교 생명자원과학대학 동물자원학과)
  • Jung, Kyung Min (Department of Animal Resources and Science, Dankook University) ;
  • Lee, Seong (Department of Animal Resources and Science, Dankook University) ;
  • Kim, Jung Woo (Department of Animal Resources and Science, Dankook University)
  • 투고 : 2013.12.19
  • 심사 : 2014.02.03
  • 발행 : 2014.03.31
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초록

본 연구는 가금에서 호흡기 질환, 심낭수종, 봉입체 간염, 산란저하증 등을 유발하는 가금 아데노바이러스의 fiber 2 유전자에 대한 특이 난황 항체를 개발하고자 실험을 실시하였다. Fiber 2 유전자를 클로닝한 뒤, 대장균 발현 시스템을 이용하여 약 22 kDa의 재조합 fiber 2 단백질을 생산하였다. 이를 산란계에 3주 간격으로 총 4회 면역하여 혈청 및 난황 내 항체가를 측정한 결과, 면역 후 12주경에 항체가가 최고치에 달하였으며, 산란계로부터 획득한 난황 항체를 이용한 Western blot analysis 결과, 가금 아데노바이러스 내 fiber 2 단백과 특이적으로 반응한다는 것을 규명하였다. 결론적으로, 가금 아데노바이러스 fiber 2에 특이적인 난황 항체 생산에 성공하였으며, 이러한 특이적인 난황 항체는 가금 아데노바이러스로 인한 질병의 예방 및 치료에 활용 가능할 것으로 사료된다.

Fowl adenovirus (FAV) is an important cause of several diseases, which result in considerable economic losses to the poultry farm. An outer capsid protein of FAV, fiber 2 is essential for virus growth, assembly or spread. This study was performed to produce about 22 kDa of recombinant fiber 2 protein and to immunize in laying hens to acquire the specific IgY antibody against the recombinant fiber 2. Laying hens were immunized with the recombinant fiber 2 intramuscularly in the breast muscle by injection 4 times at intervals of three weeks. At 12 weeks, serum- and egg yolk-antibody titers of hens against fiber 2 were increased up to 430,000 and 414,000, respectively. The recombinant fiber 2 could be recognized be the anti-His monoclonal antibody. Anti-fiber 2-IgY antibody could recognize the fiber 2 specifically in western blot analysis. These results suggested that the recombinant fiber 2 antigen could be used as an immunogen to elicit IgY antibody against fiber 2 and the anti-fiber 2-IgY could neutralize fowl adenovirus fiber 2 effectively.

키워드

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